General Cell Lines and Cell Cultures
ATCC Mouse ES Cell Basal Medium
ATCC Mouse ES Cell Basal Medium is a sterile liquid tissue culture medium containing phenol red that has been optimized to support the undifferentiated self-renewal cycle related to mouse embryonic stem cell growth. The medium contains a stable glutamine dipeptide, providing a long shelf-life when handled and stored properly. The medium may be supplemented by the addition of ES-Cell Qualified Fetal Bovine Serum (FBS) or a serum replacement designed for mouse ES cells. This medium can support the growth of mouse ES cells on either a mitotically inactivated layer of mouse embryonic fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.
Eurofins Discoverx PathHunter NCI-H460 CDC25A Degradation Cell Line, 2 vials. (93-0776C12)
The PathHunter NCI-H460 CDC25A Degradation Cell Line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting the cell cycle checkpoint pathway. This cell line enables detection of levels of CDC25a, allowing for assessment of CHK-1-activation-induced CDC25a degradation. This product uses EFC technology. The cell line included overexpresses CDC25a target protein fused to ProLabel. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of CDC25a EFC signal is lost. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/pathways.
Eurofins Discoverx InCELL Hunter A549 BTK Kinase Binding Cell Line, 2 vials. (96-0056C15)
The InCELL Hunter A549 BTK Kinase Binding Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of BTK via detection of BTK levels. This product uses EFC technology. The cell line included overexpresses BTK target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
ATCC Airway Epithelial Cell Basal Medium
Airway Epithelial Cell Basal Medium is a sterile, phenol-red-free, liquid tissue culture medium intended for use as one component in a complete ATCC Primary Cell Solutions system. This serum-free system is designed to support airway epithelial cells derived from a normal human lung. Airway Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. To support the proliferation and plating efficiency of various types of airway epithelial cells, Airway Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit. When using this complete media system, the growth of airway epithelial cells is supported without the use of feeder layers, extracellular matrix proteins, or other substrates.
ATCC MP41; Uveal Melanoma; Human (Homo sapiens)
MP41 is an epithelial-like cell line that was isolated in 2006 from the primary tumor in the uveal of a 49-year-old, female patient with melanoma. This cell line was deposited by F Nemati, Institute Curie.
ATCC Yarrowia lipolytica; IFP29
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
ATCC Toledo; Non-Hodgkin's Lymphoma; Human (Homo sapiens)
Toledo is a B lymphocyte cell line that was isolated in 1990 from the peripheral blood of a White, adult female patient with diffuse large cell lymphoma, non-Hodgkin's B cell. This cell line was deposited by H Ben-Bassat and can be used in immunology research.
ATCC Iscove's Modified Dulbecco's Medium (IMDM)
Iscove's Modified Dulbecco's Medium (IMDM) contains 4 mM L-glutamine, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate. It has applications for cell culture, cell growth, and viability.
STEMCELL Technologies Human Cord Blood CD34+ Cells, Frozen, Size: 1 x 10^6 cells
Start experiments confidently with ready-to-use, ethically sourced, human CD34+ stem and progenitor cells. With personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications, we help you get the cells you need. Isolated immunomagnetically from umbilical cord blood using positive selection and cryopreserved in serum-free medium, cells are collected using Institutional Review Board (IRB) or other regulatory authority-approved consent forms and protocols. Additional documentation to meet institutional requirements and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. CPD is added during collection as an anticoagulant. Contact techsupport@stemcell.com for donor specifications (BMI category, smoking status, ethnicity, etc.). Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. Certain products are only available in select territories.
Genscript Corporation CHO-K1/BDCA2 and FcER1G Stable
Recombinant CHO-K1 cells stably overexpress human C-type lectin domain family 4 member C (BDCA2) and Fc epsilon receptor Ig (FcER1G) on the surface The surface expression of BDCA2 is validated by FACS analysis
Eurofins Discoverx PathHunter NMU1R Total Internalization U2OS Cell Line, 2 vials. (93-0782C3)
The PathHunter NMU1R Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of nMU1R via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged nMU1R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the nMU1R receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC PC-3; Prostate Adenocarcinoma; Human (Homo sapiens)
PC-3 is a cell line initiated from a bone metastasis of a grade IV prostatic adenocarcinoma from a 62-year-old, White, male.
ATCC Salmonella enterica; subsp. enterica; serovar Abony
Salmonella enterica subsp. enterica serovar Abony strain CCM 4518 [NBRC 100797, NCTC 6017, CIP 80.39] is a whole-genome sequenced strain with applications in enteric disease research and zoonotic disease research.