General Cell Lines and Cell Cultures
Eurofins Discoverx Ready-to-Assay D1 Dopamine Receptor Frozen Cells, 2 Vials of Cells. (HTS102RTA)
The Ready-to-Assay D1 Dopamine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. dopamine) based activation of DRD1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses DRD1 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of DRD1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen β2 Adrenoceptor Receptor Stable Cell Line, 2 vials. (HTS073C)
The ChemiScreen β2 Adrenoceptor Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRB2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRB2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of ADRB2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Biochain Institute Inc Paraffin Tissue Section - Human Diabetic Diseased Tissue: Pancreas, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from single human diseased sample. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Eurofins Discoverx PathHunter CHO-K1 CHRM5 β-Arrestin Cell Line, 2 vials. (93-0519C2)
The PathHunter CHRM5 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. oxotremorine) based activation of CHRM5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM5 and EA-tagged β-Arrestin-2. Activation of CHRM5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS mFCGR2B Clustering Cell Line, 2 vials. (93-1139C3)
The Path Hunter mFcyRIIb clustering cell line is a stable, engineered cell line designed to increase signal produced from a Checkpoint Cell Line via antibody-mediated crosslinking of the receptors. This product is a companion product used to determine cross-linking based increases in potency of agonist antibodies. The cell line included overexpresses mFcyRIIb Fcg receptor. When co-cultured with a Checkpoint Cell Line in the presence of agonist antibody, the Fcg receptor binds to the antibody, inducing receptor clustering. The induced receptor clustering leads to increased activation and downstream signaling, leading to increased EFC signal production. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/checkpoint.
Eurofins Discoverx PathHunter CHO-K1 rCHRM4 β-Arrestin Cell Line, 2 vials. (93-0870C2)
The PathHunter rCHRM4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. acetylcholine) based activation of rCHRM4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rCHRM4 and EA-tagged β-Arrestin-2. Activation of rCHRM4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx ChemiScreen CCK1 Cholecystokinin Receptor Membrane Preparation, 1 vial. (HTS184M)
The ChemiScreen CCK1 Cholecystokinin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CCKAR surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Sartorius Human Multi Ab Internalization
No wash protocol- fast easy antibody-labeling method optimal for screening antibody internalization.High throughput evaluation of antibody internalization- Assay designed to measure internalization of a large number of antibodies in 96- and 384-well plate formats using 10 L of cells and 10 L of labeled antibody kit .Simultaneously measure antibody internalization cell specificity and cell health within the same well.PrincipleAntibodies are easily labeled with Intellicyt Antibody Internalization Reagent a novel pH sensitive dye.
ABclonal Technology CALCR Rabbit pAb
This gene encodes a high affinity receptor for the peptide hormone calcitonin and belongs to a subfamily of seven transmembrane-spanning G protein-coupled receptors. The encoded protein is involved in maintaining calcium homeostasis and in regulating osteoclast-mediated bone resorption. Polymorphisms in this gene have been associated with variations in bone mineral density and onset of osteoporosis. Alternate splicing results in multiple transcript variants.
ATCC Quantitative Genomic DNA from; Aspergillus flavus; Strain: SN 3
Quantitative Genomic DNA from Asperigillus flavus strain SN 3 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine fungal load.
ABclonal Technology CCR9 Rabbit pAb
The protein encoded by this gene is a G protein-coupled receptor with seven transmembrane domains that belongs to the beta chemokine receptor family. Chemokines and their receptors are key regulators of thymocyte migration and maturation in normal and inflammation conditions. This gene is differentially expressed in T lymphocytes of the small intestine and colon, and its interaction with chemokine 25 contributes to intestinal intra-epithelial lymphocyte homing to the small intestine. This suggests a role for this gene in directing immune responses to different segments of the gastrointestinal tract. This gene and its exclusive ligand, chemokine 25, are overexpressed in a variety of malignant tumors and are closely associated with tumor proliferation, apoptosis, invasion, migration and drug resistance. This gene maps to the chemokine receptor gene cluster. Multiple transcript variants encoding different isoforms have been found for this gene.
Eurofins Discoverx cAMP Hunter CHO-K1 HRH3 Gi Cell Line, 2 vials. (95-0103C2)
The cAMP Hunter HRH3 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. histamine) based activation of HRH3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type HRH3, a naturally Gi/Go coupled GPCR. Upon activation of HRH3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Amsbio LLC Fluorescent labeled Exosome Standards (bodipy)
Green fluorescent labeled exosomes (bodipy)
Biochain Institute Inc Paraffin Tissue Section - Human Bone Tumor: Chondroblastic Osteosarcoma, 5 sides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's formalin fixed paraffin embedded (FFPE) tissue section line encompasses a large selection of tissue sections which are suitable for immunohistochemistry and in situ hybridization assays. The tissue sections are approximately 5 um thick. Each slide contains tissue from a single human tumor. Documentation of tissues' clinical histories may be available upon request. For those customers who want to perform FFPE DNA and RNA isolation, BioChain can provide tissue section slices in tubes instead of mounting them on glass slides.
Biolegend MojoSort™ anti-Biotin Nanobeads; Reactivity: Species independent, all species; Apps: Cell Sep - Pos, Cell Sep - Neg; Size: 1 mL
MojoSort™ anti-Biotin Nanobeads; Reactivity: Species independent, all species; Apps: Cell Sep - Pos, Cell Sep - Neg; Size: 1 mL