General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter CHO-K1 GPR142 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0413C2A)
The PathHunter GPR142 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR142 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR142 and EA-tagged β-Arrestin-2. Activation of GPR142 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Brainxell Inc Microglia (Human iPSC-Derived, Male Line, 2M)
Microglia derived from iPSC line WC-30, male. Contents: 1 vial of ≥2x10^6 viable cryopreserved cells. Marker Expression: Confirmed expression of microglia surface markers including: CD45, CD11b, CX3CR1, P2RY12, and TMEM119. Immunocytochemistry shortly after plating microglia (shown here with a DAPI stain) reveals prominent expression of TREM2 (green) and IBA1 (red). Function: Human microglia demonstrate phagocytosis and can be stimulated to secrete a variety of cytokines. iPSC derived microglia are immunologically naive. Related diseases: Autism, Epilepsy, Multiple Sclerosis, Alzheimer's & Huntington's Disease.
Eurofins Discoverx PathHunter U2OS EphA5 Functional Assay, 2 vials. (93-0840C3)
The PathHunter U2OS EphA5 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated EphA5 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged EphA5 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx KILR HepG2 Cell Pool, 2 vials. (97-1032P044)
The KILR HepG2 cell pool is a stable, engineered target cell pool, which closely reflects the heterogeneous native protein expression of tumor environments, used to measure direct cell death mediated by immunooncology treatments (e.g. checkpoint modulators, bispecifics, CAR-T, etc.) via Antibody Dependent Cell-mediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity (CDC), and Antibody Dependent Cell Phagocytosis (ADCP). This product uses EFC technology. The cell pool is a heterogeneous population of HepG2 cells that overexpress a ProLabel-tagged housekeeping gene. Upon effector cells-mediated cell lysis, ProLabel is released to the media, where EA in the detection kit produces an EFC signal proportional to the level of ProLabel released. This allows for calculation of percent lysis of the KILR cell pool. This stable cell pool is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kilr.
Eurofins Discoverx ChemiScreen M1 Muscarinic Acetylcholine Receptor Membrane Preparation, 1 vial. (HTS044M)
The ChemiScreen M1 Muscarinic Acetylcholine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CHRM1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen LPA3 Lysophospholipid Receptor Stable Cell Line, 2 vials. (HTS130C)
The ChemiScreen LPA3 Lysophospholipid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. LPA) based activation of LPAR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses LPAR3 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of LPAR3, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx PathHunter CHO-K1 GPR139 β-Arrestin Cell Line, 2 vials. (93-0954C2)
The PathHunter GPR139 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. TC-O 9311) based activation of GPR139 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR139 and EA-tagged β-Arrestin-2. Activation of GPR139 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS IL12RB1/IL12RB2 Dimerization Cell Line, 2 vials. (93-1041C3)
The PathHunter IL12RB1/IL12RB2 Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. IL-12) induced dimerization of IL12RB1/IL12RB2 receptors. This product uses EFC technology. The cell line included overexpresses IL12RB2 target protein fused to PK alongside IL12RB1 target protein fused to EA. Upon ligand-induced dimerization of IL12RB1/IL12RB2, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/interleukins.
Eurofins Discoverx Ready-to-Assay SST2 Somatostatin Receptor Frozen Cells, 2 Vials of Cells. (HTS028RTA)
The Ready-to-Assay SST2 Somatostatin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. somatostatin) based activation of SSTR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses SSTR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of SSTR2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx PathHunter CHO-K1 CALCR (No RAMP) β-Arrestin Cell Line, 2 vials. (93-0566C2)
The PathHunter CALCR (No RAMP) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. calcitonin) based activation of CALCR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CALCR and EA-tagged β-Arrestin-2. Activation of CALCR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Genscript Corporation CHO-K1/G15/CCR1
C-C chemokine receptor type 1 (CCR1) is a G protein-coupled receptor member of the CC chemokine subfamily of receptors Following interaction with its specific chemokine ligands like macrophage inflammatory protein 1 alpha (MIP-1 alpha) regulated on activation normal T expressed and secreted protein (RANTES) and monocyte chemoattractant protein 3 (MCP-3) it triggers the onset of a process known as chemotaxis GenScript s human CCR1-expressing stable subline is guaranteed to function properly in the calcium flux assay
Biochain Institute Inc Multiple Tumor Tissue Array - 47 different tumors and their corresponding normal tissues, plus 2 control spots, 5 slides/PK
BioChain's tissue arrays or tissue microarrays (TMAs) are a useful tool in biomarker research and drug target validation. It consists of paraffin blocks that are assembled in array fashion to allow multiplex histological analysis. BioChain's TMAs provide a valuable tool for analysis of protein, RNA, or DNA molecules in oncology, both the malignant and metastatic markers. TMAs can also be constructed with tissues from other diseases and species.
Eurofins Discoverx ChemiScreen IP1 Prostanoid Receptor Stable Cell Line, 2 vials. (HTS131C)
The ChemiScreen IP1 Prostanoid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin I2) based activation of PTGIR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGIR alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of PTGIR, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Biochain Institute Inc Frozen Tissue Section - Lupus: Pancreas, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.