General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter CHO-K1 mMCHR1 β-Arrestin Cell Line, 2 vials. (93-0479C2)
The PathHunter mMCHR1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. MCH) based activation of mMCHR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mMCHR1 and EA-tagged β-Arrestin-2. Activation of mMCHR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx Ready-to-Assay NMU1 Neuromedin U Receptor Frozen Cells, 2 Vials of Cells. (HTS062RTA)
The Ready-to-Assay nMU1 Neuromedin U Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. Neuromedin U) based activation of nMU1R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses nMU1R alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of nMU1R, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx PathHunter U2OS IL12RB1/IL12RB2 Dimerization Cell Line, 2 vials. (93-1041C3)
The PathHunter IL12RB1/IL12RB2 Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. IL-12) induced dimerization of IL12RB1/IL12RB2 receptors. This product uses EFC technology. The cell line included overexpresses IL12RB2 target protein fused to PK alongside IL12RB1 target protein fused to EA. Upon ligand-induced dimerization of IL12RB1/IL12RB2, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/interleukins.
Biochain Institute Inc Matched Pair - cDNA - Human Primary and Matched Metastatic Tumor Tissue: Colon, 10 x2 rxn /PK
BioChain's human cDNA matched pair products include: Primary Pair (PP), Primary and Metastasis Pair (PM). PP consists of cDNA prepared from primary tumor and its adjacent normal tissue; PM consists of cDNA prepared from primary tumor and corresponding metastatic tumor. cDNAs in each pair are prepared from the same donor. This product line is designed for identifying tumor-specific (or disease specific) genes and tumor metastatic genes.
ATCC Cryptosporidium parvum; Quantitative DNA
Quantitative Genomic DNA from Cryptosporidium parvum can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine protozoan load and for other molecular biology applications.
ATCC HUPEC 003 FSGS G0/G0; Epithelium; Human (Homo sapiens)
For-profit organizations: For every order of this item, you must provide a signed Material Transfer Agreement Addendum for Screening Application. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item.Email the signed addendum to SalesPermits@atcc.org with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. Additional fees may apply if this product is being used for a screening use (ATCC ACS-2103F), and these fees will be applied after your order is confirmed. If you need assistance with your order, please contact our Customer Care team.
ATCC Quantitative Genomic DNA; from Legionella pneumophila; subsp. pneumophila; Strain: Philadelphia-1
Quantitative genomic DNA from Legionella pneumophila subsp. pneumophila strain Philadelphia-1 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Amsbio LLC TLR2/NF-kB Leeporter™ Luciferase Reporter-HEK293 Cell Line
TLR2/NF-kB Leeporter™ Luciferase Reporter-HEK293 Cell Line
Eurofins Discoverx PathHunter CHO-K1 DRD4 β-Arrestin Cell Line, 2 vials. (93-0690C2)
The PathHunter DRD4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. dopamine) based activation of DRD4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DRD4 and EA-tagged β-Arrestin-2. Activation of DRD4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter HCRTR1 Total GPCR Internalization U2OS Cell Line, 2 vials. (93-0729C3)
The PathHunter HCRTR1 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of HCRTR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged HCRTR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the HCRTR1 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter HEK 293 PTGER4 β-Arrestin Cell Line, 2 vials. (93-0304C1)
The PathHunter PTGER4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGER4 and EA-tagged β-Arrestin-2. Activation of PTGER4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx Ready-to-Assay EP4 Prostanoid Receptor Frozen Cells, 2 Vials of Cells. (HTS142LRTA)
The Ready-to-Assay EP4 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin E2) based activation of PTGER4 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER4 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER4, the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen FFAR3/GPR41 Free Fatty Acid Receptor Stable Cell Line, 2 vials. (HTS135C)
The ChemiScreen FFAR3/GPR41 Free Fatty Acid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. free fatty acids) based activation of FFAR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR3 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of FFAR3, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay FFA3/GPR41 Free Fatty Acid Receptor Frozen Cells, 2 Vials of Cells. (HTS135RTA)
The Ready-to-Assay FFA3/GPR41 Free Fatty Acid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. free fatty acids) based activation of FFAR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR3 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of FFAR3, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen GABAB Receptor Membrane Preparation, 1 vial. (HTS119M)
The ChemiScreen GABAB Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of GABBR1 - GABBR2 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.