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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,275)
Mouse Secondary Antibodies (14,507)
Rabbit Secondary Antibodies (6,327)
Donkey Secondary Antibodies (4,321)
Rat Secondary Antibodies (1,240)
Sheep Secondary Antibodies (703)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Horse Secondary Antibodies (76)
Pig Secondary Antibodies (76)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (55)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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Keyword Search:
1,606 – 1,620 of 72,126 results
1,606

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Target Species Rabbit
Host Species Donkey
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.50mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Rabbit IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG and light chains on all rabbit immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep.
1,607

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 680 (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Rabbit
Host Species Donkey
Conjugate DyLight 680
Applications Dot Blot,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:10000, Immunocytochemistry/Immunofluorescence 1:5000, Dot Blot, Fluorophore-linked immunosorbent assay 1:20000
Classification Polyclonal
Immunogen Rabbit IgG whole molecule
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.
1,608

Donkey anti-Human IgG (H+L) Secondary Antibody (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Antigen IgG (H+L)
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Purification Method Affinity Purified
Conjugate Unconjugated
Target Species Human
Host Species Donkey
Applications ELISA,Immunohistochemistry,Western Blot
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free with 0.01% Sodium Azide
Classification Polyclonal
Immunogen Human IgG whole molecule
Isotype IgG
Primary or Secondary Secondary
1,609

Invitrogen™ Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight™ 350

Donkey Polyclonal Secondary Antibody

Content And Storage 4°C
Target Species Rabbit
Host Species Donkey
Conjugate DyLight 350
Applications Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunocytochemistry
Form Liquid
Isotype IgG
Concentration 0.5 mg/mL
Antigen Rabbit IgG (H+L) Cross-Adsorbed
Regulatory Status RUO
Purification Method Antigen affinity chromatography
Product Type Secondary Antibody
Formulation PBS with 0.2% BSA and 0.09% sodium azide; pH 6.8 to 7.4
Immunogen Rabbit IgG-heavy and light chain.
Classification Polyclonal
Primary or Secondary Secondary
1,611

Invitrogen™ F(ab')2-Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, FITC

F(ab')2-Donkey Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Target Species Mouse
Host Species Donkey
Conjugate FITC
Applications Flow Cytometry,Immunocytochemistry
Form Liquid
Isotype IgG
Concentration 1.5 mg/mL
Antigen Mouse IgG (H+L) Highly Cross-Adsorbed
Regulatory Status RUO
Purification Method Affinity Chromatography
Product Type Secondary Antibody
Formulation PBS with 1% BSA and 0.05% sodium azide; pH 7.8
Immunogen Gamma Immunoglobins Heavy and Light chains.
Classification Polyclonal
Primary or Secondary Secondary
1,612

Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Sheep
Host Species Donkey
Conjugate Unconjugated
Applications Chemiluminescence Immunoassay (CIA),ELISA,Flow Cytometry,Immunoassay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunomicroscopy,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 4.5mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Immunoassay
Classification Polyclonal
Immunogen Purified Sheep IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on sheep IgG and light chains on all sheep immunoglobulins. This antibody has been pre-adsorbed against human and rabbit IgG.
1,614

Donkey anti-Goat IgG (H+L) Secondary Antibody [Janelia Fluor 525], Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Goat
Host Species Donkey
Conjugate Janelia Fluor 525
Applications Western Blot,ELISA,Immunohistochemistry,Immunocytochemistry/Immunofluorescence,Immunohistochemistry (Paraffin),Immunohistochemistry (Frozen)
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against goat IgG.
Primary or Secondary Secondary
1,615

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Biotin, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Donkey
Conjugate Biotin
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation PBS
Classification Polyclonal
Immunogen This Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against rabbit IgG.
Primary or Secondary Secondary
1,616

Invitrogen™ Donkey anti-Mouse IgG (H+L) Secondary Antibody, AP

Donkey Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Species Mouse
Host Species Donkey
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Western Blot
Form Liquid
Isotype IgG
Concentration 1.5 mg/mL
Antigen Mouse IgG (H+L)
Regulatory Status RUO
Purification Method Affinity chromatography
Product Type Secondary Antibody
Formulation Secondary antibody with 30mM triethanolamine, 1% BSA, 5mM MgCl2, 0.1mM ZnCl2 and 0.05% sodium azide; pH 7.2
Immunogen Gamma Immunoglobins Heavy and Light chains.
Classification Polyclonal
Primary or Secondary Secondary
1,617

Invitrogen™ Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 555

Donkey Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage 4°C, store in dark
Target Species Rat
Host Species Donkey
Conjugate Alexa Fluor Plus 555
Applications Immunocytochemistry
Form Liquid
Isotype IgG
Concentration 2 mg/mL
Antigen Rat IgG (H+L) Highly Cross-Adsorbed
Regulatory Status RUO
Purification Method Affinity chromatography
Product Type Secondary Antibody
Formulation Proprietary buffer with 0.016% Methylisothiazolone, 0.016% Bromonitrodioxane; pH 6.5
Immunogen Gamma Immunoglobins Heavy and Light chains.
Classification Polyclonal
Primary or Secondary Secondary
1,618

Donkey anti-Goat IgG (H+L) Secondary Antibody, FITC, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Goat
Host Species Donkey
Conjugate FITC
Applications Flow Cytometry,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Immunohistochemistry (Paraffin)
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:200 - 1:2, Immunohistochemistry-Paraffin 1:200 - 1:2
Classification Polyclonal
Immunogen Goat IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
1,619
Promotions Available

anti-Rabbit IgG, peroxidase-linked species-specific whole antibody (from donkey) Secondary Antibody, Cytiva
GSA_VA

A highly species-specific anti-Rabbit IgG. Cytiva Lifescience™ anti-Rabbit IgG, peroxidase-linked species-specific whole antibody (from donkey) Secondary Antibody is used with Amersham™ ECL Western Blotting detection reagents.

Antigen IgG
Purification Method Affinity Purified
Host Species Donkey
Conjugate HRP
Classification Monoclonal
Isotype IgG
Primary or Secondary Secondary
1,620

Donkey anti-Sheep IgG (H+L) Secondary Antibody, DyLight 800 (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Sheep
Host Species Donkey
Conjugate DyLight 800
Applications Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:10000, Immunocytochemistry/Immunofluorescence 1:5000, Fluorophore-linked immunosorbent assay 1:20000
Classification Polyclonal
Immunogen Sheep IgG whole molecule
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit or Rat Serum Proteins. This antibody will react with heavy chains of Sheep IgG and with light chains of most Sheep immunoglobulins.