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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,277)
Mouse Secondary Antibodies (14,573)
Rabbit Secondary Antibodies (6,327)
Donkey Secondary Antibodies (4,321)
Rat Secondary Antibodies (1,242)
Sheep Secondary Antibodies (703)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Pig Secondary Antibodies (77)
Horse Secondary Antibodies (76)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (57)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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1,936 – 1,950 of 72,198 results
1,936

Donkey anti-Rabbit IgG (H+L) Secondary Antibody [Janelia Fluor 525], Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Donkey
Conjugate Janelia Fluor 525
Applications Western Blot,Flow Cytometry,ELISA,Immunohistochemistry,Immunocytochemistry/Immunofluorescence,Immunoprecipitation,Immunohistochemistry (Frozen),Immunodiffusion,Immunoassay
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against purified rabbit IgG (H&L).
Primary or Secondary Secondary
1,937

Donkey anti-Rabbit IgG Fc Secondary Antibody, HRP, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Rabbit
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Concentration LYOPH
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000 - 1:10000, ELISA 1:10000 - 1:50000, Immunohistochemistry 1:500 - 1:2500
Classification Polyclonal
Immunogen Rabbit IgG F(c) fragment
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against Rabbit IgG F(ab).
1,939

Donkey anti-Sheep IgG (H+L) Secondary Antibody, HRP, Novus Biologicals™
SDP

Donkey Polyclonal Antibody has been used in 3 publications

Content And Storage Store at 4C. Do not freeze.
Target Species Sheep
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:5000-1:50000, ELISA 1:10000-1:100000, Immunohistochemistry 1:200 - 1:5000, Immunocytochemistry/Immunofluorescence 1:200 - 1:5000, Immunohistochemistry-Paraffin 1:200 - 1:5000
Classification Polyclonal
Immunogen Sheep IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with sheep IgG and with light chains common to other sheep immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
1,940

Donkey anti-Human IgG (H+L) Secondary Antibody, DyLight 405, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Human
Host Species Donkey
Conjugate DyLight 405
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50 mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Human IgG (H+L) Secondary Antibody was developed against human IgG-heavy and light chain.
Primary or Secondary Secondary
1,941

Donkey anti-Sheep IgG (H+L) Secondary Antibody, Allophycocyanin, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Sheep
Host Species Donkey
Conjugate APC
Applications ELISA,Immunoassay,Immunodiffusion,Immunohistochemistry,Immunoprecipitation,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Formulation PBS
Classification Polyclonal
Immunogen This Donkey anti-Sheep IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against purified sheep IgG (H&L).
Primary or Secondary Secondary
1,942

Donkey anti-Human IgG (H+L) Secondary Antibody, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Antigen IgG (H+L)
Content And Storage Store at 4°C.
Purification Method Affinity Purified
Conjugate Unconjugated
Target Species Human
Host Species Donkey
Applications ELISA,Immunohistochemistry,Western Blot
Formulation 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml BSA- Immunoglobulin and Protease free with 0.01% Sodium Azide
Classification Polyclonal
Immunogen Human IgG whole molecule
Isotype IgG
Primary or Secondary Secondary
1,943

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Rabbit
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Simple Western,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200 - 1:5000, ELISA 1:200 - 1:5000, Immunohistochemistry 1:500 - 1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen This Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) was developed against purified rabbit IgG (H+L).
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) heavy gamma chains on rabbit IgG and light chains on all rabbit immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep.
1,944
Promotions Available

Goat IgG VisUCyte HRP Polymer, R&D Systems™
SDP

Donkey Polyclonal Antibody has been used in 2 publications

Content And Storage 6 months from date of receipt, 2 to 8 degreesC as supplied.
Host Species Donkey
Conjugate HRP Polymer
Applications Immunohistochemistry
Isotype IgG
Antigen IgG
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Immunohistochemistry
Gene Alias Immunoglobulin G, ImmunoglobulinG
Gene ID (Entrez) 3500
Formulation Supplied as a solution in PBS with preservative.
Immunogen Goat IgG
Classification Polyclonal
Primary or Secondary Secondary
Test Specificity Detects heavy ( gamma ) chains on goat IgG and light chains on all goat immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin goat serum proteins or serum proteins from human, mouse or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
1,945

Donkey anti-Sheep IgG (H+L) Secondary Antibody, DyLight 488 (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Sheep
Host Species Donkey
Conjugate DyLight 488
Applications Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:10000, Immunocytochemistry/Immunofluorescence 1:5000, Fluorophore-linked immunosorbent assay 1:20000
Classification Polyclonal
Immunogen Sheep IgG whole molecule
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit or Rat Serum Proteins. This antibody will react with heavy chains of Sheep IgG and with light chains of most Sheep immunoglobulins.
1,946

Donkey anti-Human IgG (H+L) Secondary Antibody, DyLight 680, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Human
Host Species Donkey
Conjugate DyLight 680
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Human IgG (H+L) Secondary Antibody was developed against human IgG-heavy and light chain.
Primary or Secondary Secondary
1,947

Donkey anti-Rat IgG (H+L) Secondary Antibody [Janelia Fluor 669], Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rat
Host Species Donkey
Conjugate Janelia Fluor 669
Applications Western Blot,ELISA,Immunohistochemistry
Form Purified
Isotype IgG
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Rat IgG whole molecule
Primary or Secondary Secondary
1,948

Donkey anti-Mouse IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Mouse
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Mouse IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat and sheep IgG.
1,950

Donkey anti-Sheep IgG (H+L) Secondary Antibody, DyLight 405, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Sheep
Host Species Donkey
Conjugate DyLight 405
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Sheep IgG (H+L) Secondary Antibody was developed against sheep IgG-heavy and light chain.
Primary or Secondary Secondary