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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,254)
Mouse Secondary Antibodies (14,502)
Rabbit Secondary Antibodies (6,318)
Donkey Secondary Antibodies (4,321)
Rat Secondary Antibodies (1,238)
Sheep Secondary Antibodies (703)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Horse Secondary Antibodies (76)
Pig Secondary Antibodies (76)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (57)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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2,566 – 2,580 of 72,091 results
2,566

Rabbit anti-Goat IgG Fc Secondary Antibody, PE, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Goat
Host Species Rabbit
Conjugate PE
Applications Chemiluminescence Immunoassay (CIA),ELISA,Flow Cytometry,Immunoassay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunomicroscopy,Western Blot
Form Purified
Isotype IgG
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity purified
Formulation PBS
Classification Polyclonal
Immunogen Purified Goat IgG Fc fragment
Primary or Secondary Secondary
2,567

Rabbit anti-Goat IgG (H+L) Secondary Antibody, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody has been used in 6 publications

Content And Storage Store at 4C in the dark.
Target Species Goat
Host Species Rabbit
Conjugate Unconjugated
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000-1:30000, ELISA Coating - 1:100-1:500 Primary - 1:1000-1:30000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:200- 1:2000, Immunohistochemistry-Paraffin 1:200- 1:2000
Classification Polyclonal
Immunogen Goat IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
2,568

Rabbit anti-Mouse IgG3 Heavy Chain Secondary Antibody, DyLight 488, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Mouse
Host Species Rabbit
Conjugate DyLight 488
Applications ELISA,Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot
Research Discipline Immunology
Antigen IgG3
Gene Symbols IGHG3
Purification Method Affinity Purified
Dilution Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
Gene Alias DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, IgG3, immunoglobulin heavy constant gamma 3 (G3m marker), MGC45809
Gene ID (Entrez) 3502
Formulation 50 mM Sodium Borate
Classification Polyclonal
Immunogen Mouse IgG3 heavy chain
Test Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum and Mouse IgG.
2,569

Rabbit anti-Guinea Pig IgG Fc Secondary Antibody, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Guinea Pig
Host Species Rabbit
Conjugate Unconjugated
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Concentration 1.69 mg/mL
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000
Classification Polyclonal
Immunogen Guinea Pig IgG F(c) fragment
Primary or Secondary Secondary
Test Specificity No reaction was observed against Guinea Pig IgG F(ab) .
2,570

Rabbit F(ab')2 anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed), Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Goat
Host Species Rabbit
Conjugate Unconjugated
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000
Classification Polyclonal
Immunogen Goat IgG whole molecule
Primary or Secondary Secondary
Test Specificity No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
2,571

Rabbit anti-Sheep IgG (H+L) Secondary Antibody, DyLight 800, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Sheep
Host Species Rabbit
Conjugate DyLight 800
Applications Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:10000-1:25000, Immunocytochemistry/Immunofluorescence 1:5000, Fluorophore-linked immunosorbent assay 1:20000
Classification Polyclonal
Immunogen Sheep IgG whole molecule
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody will react with heavy chains of Sheep IgG and with light chains of most Sheep immunoglobulins.
2,572

Rabbit anti-Equine IgG (H+L) Secondary Antibody, Rhodamine, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species Equine
Host Species Rabbit
Conjugate Rhodamine
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG
Regulatory Status RUO
Purification Method Multi-step
Dilution Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Fluorophore-linked immunosorbent assay 1:100 - 1:2000
Gene Alias immunoglobulin heavy constant gamma 1 (G1m marker)
Classification Polyclonal
Immunogen Horse IgG whole molecule
Primary or Secondary Secondary
Test Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Horse IgG and Horse Serum.
2,573

Rabbit anti-Goat IgG (H+L) Secondary Antibody, Biotin, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Goat
Host Species Rabbit
Conjugate Biotin
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:20000 - 1:400000, ELISA 1:20000 - 1:400000, Immunohistochemistry 1:500 - 1:5000, Immunocytochemistry/Immunofluorescence 1:200 - 1:1000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Goat IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
2,574

Invitrogen™ Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody

Rabbit Recombinant Superclonal Secondary Antibody

Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Species Mouse
Host Species Rabbit
Conjugate Unconjugated
Applications ELISA,Western Blot,Immunocytochemistry
Form Liquid
Isotype IgG
Concentration 1 mg/mL
Antigen Mouse IgG (H+L)
Regulatory Status RUO
Purification Method Protein A
Product Type Secondary Antibody
Formulation PBS with 0.09% sodium azide; pH 7.2-7.4
Immunogen Recombinant full-length protein.
Classification Recombinant Superclonal
Primary or Secondary Secondary
2,575

Invitrogen™ Rabbit anti-Bovine IgG (H+L) Secondary Antibody, Texas Red

Rabbit Polyclonal Secondary Antibody

Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Target Species Bovine
Host Species Rabbit
Conjugate Texas Red
Applications Dot Blot,ELISA,Flow Cytometry,Immunocytochemistry
Form Liquid
Isotype IgG
Concentration 2 mg/mL
Antigen Bovine IgG (H+L)
Regulatory Status RUO
Purification Method IgG fraction
Product Type Secondary Antibody
Formulation PBS with 1% BSA and 0.01% sodium azide; pH 7.2
Immunogen Bovine IgG whole molecule.
Classification Polyclonal
Primary or Secondary Secondary
2,576

Rabbit anti-Chicken IgG F(ab')2 Secondary Antibody, FITC, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C in the dark.
Target Species Chicken
Host Species Rabbit
Conjugate Fluorescein
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Concentration LYOPH
Antigen IgG F(ab')2
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot, Flow Cytometry 1:500 - 1:2500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Gene Alias immunoglobulin heavy constant gamma 1 (G1m marker)
Classification Polyclonal
Immunogen Chicken IgG F(ab')2 fragment
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against Chicken IgG F(c).
2,577

Rabbit anti-Sheep IgG (H+L) Secondary Antibody, Allophycocyanin, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Sheep
Host Species Rabbit
Conjugate APC
Applications Dot Blot,ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation PBS
Classification Polyclonal
Immunogen Sheep IgG whole molecule
Primary or Secondary Secondary
2,578

Rabbit anti-Human IgA Heavy Chain Secondary Antibody, Texas Red, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Human
Host Species Rabbit
Conjugate Texas Red
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence
Isotype IgA
Concentration LYOPH
Antigen IgA Heavy Chain
Gene Symbols IGHA1
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Gene Alias Immunoglobulin A
Gene ID (Entrez) 3493
Classification Polyclonal
Immunogen Human IgA heavy chain
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity Specificity was confirmed by ELISA minimal against other Human heavy or light chain combinations.
2,579

Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody, Texas Red, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Mouse
Host Species Rabbit
Conjugate Texas Red
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG1
Concentration LYOPH
Antigen IgG1 Heavy Chain
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000 - 1:10000, Flow Cytometry 1:500 - 1:2500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification Polyclonal
Immunogen Mouse IgG1 heavy chain
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
2,580

Rabbit anti-Canine IgG F(ab')2 Secondary Antibody, Novus Biologicals™
SDP

Rabbit Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.
Target Species Canine
Host Species Rabbit
Conjugate Unconjugated
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Concentration LYOPH
Antigen IgG F(ab')2
Regulatory Status RUO
Purification Method Delipidation and Defibrination
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000
Gene Alias immunoglobulin heavy constant gamma 1 (G1m marker)
Classification Polyclonal
Immunogen Dog IgG F(ab')2 fragment, rabbit anti Dog Fab2
Reconstitution Reconstitute with 2.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against Dog IgG F(c).