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Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species
Human
Host Species
Mouse
Conjugate
Unconjugated
Applications
Western Blot,ELISA
Form
Purified
Isotype
IgG1
Concentration
1.0 mg/mL
Gene Symbols
IGHG1
Regulatory Status
RUO
Purification Method
Protein G purified
Dilution
Western Blot 1:500 - 1:2000, ELISA 1:10000
Gene Alias
immunoglobulin heavy constant gamma 1 (G1m marker)
Gene ID (Entrez)
3500
Immunogen
Human IgG was isolated from human sera and purified by chromatography.
Classification
Monoclonal
Primary or Secondary
Secondary
Test Specificity
Monoclonal anti-Human IgG is derived from a hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, and has no cross reactivity with human IgM molecule as determined by an ELISA.