Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).

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1,321 – 1,335 of 73,088 results

1,321

Donkey anti-Rat IgG (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Target Species	Rat
Host Species	Donkey
Conjugate	Alkaline Phosphatase (AP)
Applications	ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Concentration	1.50mg/mL
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Affinity purified
Dilution	Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification	Polyclonal
Immunogen	Purified Rat IgG (H&L)
Primary or Secondary	Secondary
Test Specificity	Based on IEP, this antibody reacts with heavy gamma chains on rat IgG and light chains on all rat immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit and sheep IgG.

1,322

Donkey anti-Sheep IgG (H+L) Secondary Antibody, FITC, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species	Sheep
Host Species	Donkey
Conjugate	FITC
Applications	Dot Blot,Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Affinity Purified
Dilution	Western Blot 1:250 - 1:500, Flow Cytometry 1:500 - 1:2500, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Immunohistochemistry-Paraffin 1:200 - 1:500, Dot Blot, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification	Polyclonal
Immunogen	Sheep IgG whole molecule
Primary or Secondary	Secondary
Test Specificity	Prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Sheep IgG and Sheep Serum.

1,323

Donkey anti-Goat IgG (H+L) Secondary Antibody, DyLight 550, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C in the dark.
Target Species	Goat
Host Species	Donkey
Conjugate	DyLight 550
Applications	Adhesion Activation,ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Immunohistochemistry (Paraffin),Western Blot
Form	Purified
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Immunogen affinity purified
Formulation	50mM Sodium Borate
Classification	Polyclonal
Immunogen	This Donkey anti-Goat IgG (H+L) Secondary Antibody was developed against goat IgG-heavy and light chain.
Primary or Secondary	Secondary

1,324

Invitrogen™ F(ab')2-Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, FITC

F(ab')2-Donkey Polyclonal Secondary Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Target Species	Rat
Host Species	Donkey
Conjugate	FITC
Applications	Immunocytochemistry
Form	Liquid
Isotype	IgG
Concentration	1.5 mg/mL
Antigen	Rat IgG (H+L) Highly Cross-Adsorbed
Regulatory Status	RUO
Purification Method	Affinity Chromatography
Product Type	Secondary Antibody
Formulation	PBS with 1% BSA and 0.05% sodium azide; pH 7.8
Immunogen	Gamma Immunoglobins Heavy and Light chains.
Classification	Polyclonal
Primary or Secondary	Secondary

1,325

Donkey anti-Mouse IgG (H+L) Secondary Antibody, DyLight 650, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species	Mouse
Host Species	Donkey
Conjugate	DyLight 650
Applications	Flow Cytometry,Immunomicroscopy,Western Blot
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Concentration	LYOPH
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Affinity purified
Dilution	Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000
Classification	Polyclonal
Immunogen	Purified Mouse IgG (H&L)
Reconstitution	Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary	Secondary
Test Specificity	Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins

1,326

IgG Donkey anti-Rat, FITC, Polyclonal, Secondary Antibody, MilliporeSigma™

Donkey Polyclonal Antibody

Pricing & Availability

1,327

Donkey anti-Rabbit IgG Fc Secondary Antibody, PerCP, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C in the dark.
Target Species	Rabbit
Host Species	Donkey
Conjugate	PerCP
Applications	ELISA,Immunohistochemistry,Western Blot
Form	Purified
Isotype	IgG
Antigen	IgG Fc
Regulatory Status	RUO
Purification Method	Immunogen affinity purified
Formulation	PBS
Classification	Polyclonal
Immunogen	Rabbit IgG F(c) fragment
Primary or Secondary	Secondary

1,328

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, PerCP, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C in the dark.
Target Species	Rabbit
Host Species	Donkey
Conjugate	PerCP
Applications	ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Multi-step
Formulation	PBS
Classification	Polyclonal
Immunogen	Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with rabbit whole IgG molecule in donkey.
Primary or Secondary	Secondary

1,329

Donkey anti-Sheep IgG (H+L) Secondary Antibody, HRP, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species	Sheep
Host Species	Donkey
Conjugate	HRP
Applications	Dot Blot,ELISA,Immunohistochemistry,Western Blot
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Concentration	LYOPH
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Affinity Purified
Dilution	Western Blot 1:3000 - 1:15000, ELISA 1:50000 - 1:150000, Immunohistochemistry 1:1000 - 1:5000, Dot Blot
Classification	Polyclonal
Immunogen	Donkey anti-Sheep IgG (H+L) Secondary Antibody [HRP] was produced by repeated immunization with Sheep IgG whole molecule in donkey.
Reconstitution	Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary	Secondary
Test Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Sheep IgG and Sheep Serum.

1,330

Invitrogen™ Donkey anti-Mouse IgG (H+L) Secondary Antibody, FITC

Donkey Polyclonal Secondary Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Target Species	Mouse
Host Species	Donkey
Conjugate	FITC
Applications	Flow Cytometry,Immunocytochemistry
Form	Liquid
Isotype	IgG
Concentration	1.5 mg/mL
Antigen	Mouse IgG (H+L)
Regulatory Status	RUO
Purification Method	Affinity chromatography
Product Type	Secondary Antibody
Formulation	PBS with 1% BSA and 0.05% sodium azide; pH 7.8
Immunogen	Gamma Immunoglobins Heavy and Light chains.
Classification	Polyclonal
Primary or Secondary	Secondary

1,331

Donkey anti-Goat IgG (H+L) Secondary Antibody, PE, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C in the dark.
Conjugate	PE
Target Species	Goat
Host Species	Donkey
Applications	Dot Blot,ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry,Immunofluorescence,Immunohistochemistry,Western Blot
Form	Purified
Isotype	IgG
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Multi-step
Formulation	PBS
Classification	Polyclonal
Immunogen	Donkey anti-Goat IgG (H+L) Secondary Antibody was produced by repeated immunization with goat IgG whole molecule in donkey
Primary or Secondary	Secondary

1,332

Donkey anti-Rat IgG (H+L) Secondary Antibody, Biotin, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C in the dark.
Target Species	Rat
Host Species	Donkey
Conjugate	Biotin
Applications	ELISA,Immunohistochemistry,Western Blot
Isotype	IgG
Concentration	LYOPH
Antigen	IgG (H+L)
Purification Method	Affinity Purified
Dilution	Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000, Immunohistochemistry-Paraffin 1:1000 - 1:5000, Immunohistochemistry-Frozen 1:1000 - 1:5000
Formulation	Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Classification	Polyclonal
Immunogen	Rat IgG whole molecule
Reconstitution	Reconstitute with 1.0 mL deionized water
Test Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Rat IgG and Rat Serum.

1,333

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 550, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C in the dark.
Target Species	Rabbit
Host Species	Donkey
Conjugate	DyLight 550
Applications	ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form	Purified
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Immunogen affinity purified
Formulation	50mM Sodium Borate
Classification	Polyclonal
Immunogen	This Donkey anti-Rabbit IgG (H+L) Secondary Antibody was developed against rabbit IgG-heavy and light chain.
Primary or Secondary	Secondary

1,334

Donkey anti-Human IgG (H+L) Secondary Antibody, DyLight 650, Novus Biologicals™

Donkey Polyclonal Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C in the dark.
Target Species	Human
Host Species	Donkey
Conjugate	DyLight 650
Applications	ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form	Purified
Isotype	IgG
Research Discipline	Adaptive Immunity, Immunology
Antigen	IgG (H+L)
Regulatory Status	RUO
Purification Method	Immunogen affinity purified
Formulation	50mM Sodium Borate
Classification	Polyclonal
Immunogen	This Donkey anti-Human IgG (H+L) Secondary Antibody was developed against human IgG-heavy and light chain.
Primary or Secondary	Secondary

1,335

Invitrogen™ Donkey anti-Mouse IgG (H+L) Secondary Antibody

Donkey Polyclonal Secondary Antibody

Pricing & Availability
Specifications

Content And Storage	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Species	Mouse
Host Species	Donkey
Conjugate	Unconjugated
Applications	ELISA
Form	Liquid
Isotype	IgG
Concentration	>4.5 mg/mL
Antigen	Mouse IgG (H+L)
Regulatory Status	RUO
Purification Method	Affinity chromatography
Product Type	Secondary Antibody
Formulation	PBS with 0.05% sodium azide; pH 7.2
Immunogen	Gamma Immunoglobins Heavy and Light chains.
Classification	Polyclonal
Primary or Secondary	Secondary

Secondary Antibodies

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