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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,275)
Mouse Secondary Antibodies (14,507)
Rabbit Secondary Antibodies (6,327)
Donkey Secondary Antibodies (4,321)
Rat Secondary Antibodies (1,240)
Sheep Secondary Antibodies (703)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Horse Secondary Antibodies (76)
Pig Secondary Antibodies (76)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (55)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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1,456 – 1,470 of 72,126 results
1,456

Invitrogen™ F(ab')2-Donkey anti-Sheep IgG (H+L) Secondary Antibody, TRITC

F(ab')2-Donkey Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Target Species Sheep
Host Species Donkey
Conjugate TRITC
Applications Immunocytochemistry
Form Lyophilized
Isotype IgG
Concentration 1 mg/mL
Antigen Sheep IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Chromatography
Product Type Secondary Antibody
Formulation PBS with 1% BSA and 0.05% sodium azide; pH 7.2
Immunogen Gamma Immunoglobins Heavy and Light chains.
Classification Polyclonal
Primary or Secondary Secondary
1,457

Donkey F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody, PE (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Aliquot reconstituted liquid and store at 4°C in the dark. Do not freeze.
Target Species Mouse
Host Species Donkey
Conjugate PE
Applications Dot Blot,Flow Cytometry,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:100-1:500, Flow Cytometry 1:100 - 1:250, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:100 - 1:250, Immunohistochemistry-Paraffin 1:200-1:500, Dot Blot
Classification Polyclonal
Immunogen Donkey F(ab')2 anti-Mouse IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) was produced by repeated immunization with Mouse IgG whole molecule in donkey.
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat or Sheep Serum Proteins.
1,458

Donkey anti-Goat IgG (H+L) Secondary Antibody, HRP, Novus Biologicals™
SDP

Donkey Polyclonal Antibody has been used in 7 publications

Content And Storage Store at 4C. Do not freeze.
Target Species Goat
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000-1:30000, ELISA 1:10000-1:100000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:200 - 1:500, Immunohistochemistry-Paraffin 1:200 - 1:500, Immunohistochemistry-Frozen 1:50-1:500
Classification Polyclonal
Immunogen Goat IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins. This antibody may cross react with IgG from other species.
1,459

Donkey anti-Goat IgG (H+L) Secondary Antibody, DyLight 488, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Goat
Host Species Donkey
Conjugate DyLight 488
Applications Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:10000, Immunocytochemistry/Immunofluorescence 1:5000, Fluorophore-linked immunosorbent assay 1:20000
Classification Polyclonal
Immunogen Goat IgG, whole molecule
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.
1,460
Promotions Available

Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody, R&D Systems™
GREENER_CHOICE SDP

Donkey Polyclonal Antibody has been used in 2 publications

1,461

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Allophycocyanin, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Donkey
Conjugate APC
Applications ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation PBS
Classification Polyclonal
Immunogen Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with rabbit whole IgG molecule in donkey.
Primary or Secondary Secondary
1,462

Donkey anti-Mouse IgG (H+L) Secondary Antibody, PerCP, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Mouse
Host Species Donkey
Conjugate PerCP
Applications ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation PBS
Classification Polyclonal
Immunogen Mouse IgG whole molecule
Primary or Secondary Secondary
1,463

Invitrogen™ F(ab')2-Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, AP

F(ab')2-Donkey Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Species Mouse
Host Species Donkey
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Western Blot
Form Liquid
Isotype IgG
Concentration 1.5 mg/mL
Antigen Mouse IgG (H+L) Highly Cross-Adsorbed
Regulatory Status RUO
Purification Method Affinity Chromatography
Product Type Secondary Antibody
Formulation Secondary antibody with 1% BSA, 30mM triethanolamine, 5mM MgCl2, 0.1mM ZnCl2 and 0.05% sodium azide; pH 7.2
Immunogen Gamma Immunoglobins Heavy and Light chains.
Classification Polyclonal
Primary or Secondary Secondary
1,464

Invitrogen™ Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Qdot™ 525

Donkey Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage 4°C, store in dark
Target Species Rabbit
Host Species Donkey
Conjugate Qdot 525
Applications Flow Cytometry,Immunohistochemistry,Western Blot,Immunocytochemistry
Form Liquid
Isotype IgG
Concentration 1 μM
Antigen Rabbit IgG (H+L)
Regulatory Status RUO
Purification Method Purified
Product Type Secondary Antibody
Formulation 0.05M borate with 1M betaine and 0.05% sodium azide; pH 8.3
Immunogen Gamma Immunoglobins Heavy and Light chains.
Classification Polyclonal
Primary or Secondary Secondary
1,465

Donkey anti-Sheep IgG (H+L) Secondary Antibody, DyLight 594, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Sheep
Host Species Donkey
Conjugate DyLight 594
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Sheep IgG (H+L) Secondary Antibody was developed against sheep IgG-heavy and light chain.
Primary or Secondary Secondary
1,466

Donkey anti-Rabbit IgG Fc Secondary Antibody, HRP, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Rabbit
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Concentration LYOPH
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000 - 1:10000, ELISA 1:10000 - 1:50000, Immunohistochemistry 1:500 - 1:2500
Classification Polyclonal
Immunogen Rabbit IgG F(c) fragment
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against Rabbit IgG F(ab).
1,467

Donkey anti-Mouse IgG (H+L) Secondary Antibody, Biotin, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species Mouse
Host Species Donkey
Conjugate Biotin
Applications Dot Blot,ELISA,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Gene Symbols IGHG
Regulatory Status RUO
Purification Method Multi-step
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000, Dot Blot
Classification Polyclonal
Immunogen Mouse IgG whole molecule
Primary or Secondary Secondary
Test Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Mouse IgG and Mouse Serum.
1,468

Donkey anti-Goat IgG (H+L) Secondary Antibody, PerCP, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Goat
Host Species Donkey
Conjugate PerCP
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation PBS
Classification Polyclonal
Immunogen This Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against goat IgG.
Primary or Secondary Secondary
1,469

Donkey anti-Sheep IgG (H+L) Secondary Antibody, HRP, Novus Biologicals™
SDP

Donkey Polyclonal Antibody has been used in 3 publications

Content And Storage Store at 4C. Do not freeze.
Target Species Sheep
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:5000-1:50000, ELISA 1:10000-1:100000, Immunohistochemistry 1:200 - 1:5000, Immunocytochemistry/Immunofluorescence 1:200 - 1:5000, Immunohistochemistry-Paraffin 1:200 - 1:5000
Classification Polyclonal
Immunogen Sheep IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with sheep IgG and with light chains common to other sheep immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
1,470

Donkey anti-Goat IgG (H+L) Secondary Antibody, DyLight 594, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Goat
Host Species Donkey
Conjugate DyLight 594
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen This Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against goat IgG.
Primary or Secondary Secondary