Rabbit Secondary Antibodies
Secondary antibodies where rabbit is the host species. Secondary antibodies must be raised against the host species of the primary antibody. Includes different antigens, classifications, conjugations, and isotypes.
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Rabbit anti-Chicken IgY (H+L) Secondary Antibody, HRP, R&D Systems™
A Novus product, part of R&D Systems. Rabbit Polyclonal Antibody
| Content And Storage | Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage. |
|---|---|
| Target Species | Chicken |
| Host Species | Rabbit |
| Conjugate | HRP |
| Applications | ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgG,IgY |
| Concentration | LYOPH |
| Antigen | IgY (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin |
| Product Type | Antibody |
| Classification | Polyclonal |
| Immunogen | Purified Chicken IgG/Y (H&L) |
| Reconstitution | Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on chicken IgG/Y and light chains on all chicken immunoglobulins |
Rabbit anti-Chicken IgY (H+L) Secondary Antibody, HRP, Novus Biologicals™
Rabbit Polyclonal Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Chicken |
| Host Species | Rabbit |
| Conjugate | HRP |
| Applications | ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgY |
| Concentration | 1.0 mg/mL |
| Antigen | IgY (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:1000-1:10000, ELISA, Immunohistochemistry 1:200 - 1:5000, Immunocytochemistry/Immunofluorescence 1:200 - 1:5000, Immunohistochemistry-Paraffin |
| Classification | Polyclonal |
| Immunogen | Chicken IgG (IgY)-heavy and light chain |
| Primary or Secondary | Secondary |
| Test Specificity | By immunoelectrophoresis and ELISA this reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species. |
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Human |
| Host Species | Rabbit |
| Conjugate | Unconjugated |
| Applications | Chemiluminescence Immunoassay (CIA),ELISA,Flow Cytometry,Immunoassay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunomicroscopy,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 4.5mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Immunoassay |
| Classification | Polyclonal |
| Immunogen | Purified Human IgG (H&L) |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on human IgG and light chains on all human immunoglobulins |
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Human |
| Host Species | Rabbit |
| Conjugate | Unconjugated |
| Applications | ELISA,Immunohistochemistry,Peptide ELISA,Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Immunogen affinity purified |
| Dilution | Western Blot 1:10000 - 1:50000, ELISA 1:1000000, Immunohistochemistry 1:2000 - 1:10000, Peptide ELISA |
| Classification | Polyclonal |
| Immunogen | Human IgG whole molecule |
| Primary or Secondary | Secondary |
| Test Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum. |
| Content And Storage | 4°C |
|---|---|
| Target Species | Human |
| Host Species | Rabbit |
| Conjugate | Unconjugated |
| Applications | Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunocytochemistry |
| Form | Liquid |
| Isotype | IgG |
| Concentration | 2.31 mg/mL |
| Antigen | Human IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Antigen affinity chromatography |
| Product Type | Secondary Antibody |
| Formulation | PBS with no preservative; pH 7.6 |
| Classification | Polyclonal |
| Primary or Secondary | Secondary |
Rabbit anti-Mouse IgG1 Secondary Antibody (20) [Janelia Fluor 549], R&D Systems™
A Novus product, part of R&D Systems. Rabbit Monoclonal Antibody
| Content And Storage | Store at -20°C. Avoid freeze-thaw cycles. |
|---|---|
| Conjugate | Biotin |
| Target Species | Mouse |
| Host Species | Rabbit |
| Applications | ELISA |
| Form | Purified |
| Isotype | IgG |
| Antigen | IgM |
| Purification Method | Protein A purified |
| Gene Alias | AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH |
| Formulation | PBS, 50% Glycerol, 1% BSA with 0.09% Sodium Azide |
| Gene ID (Entrez) | 3507 |
| Classification | Monoclonal |
| Immunogen | Mouse IgM |
| Primary or Secondary | Secondary |
| Clone | RM109 |
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Goat IgG HRP-conjugated Antibody, R&D Systems™
Goat IgG HRP-conjugated Antibody, R&D Systems™
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Rabbit Polyclonal Antibody
| Content And Storage | Do not freeze. 6 months from date of receipt, 2 to 8 degreesC as supplied. |
|---|---|
| Target Species | Goat |
| Host Species | Rabbit |
| Conjugate | HRP |
| Applications | Western Blot |
| Isotype | IgG |
| Antigen | IgG |
| Gene Symbols | IGHG1 |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot |
| Gene Alias | Immunoglobulin G, ImmunoglobulinG |
| Product Type | Antibody |
| Gene ID (Entrez) | 3500 |
| Immunogen | Goat IgG |
| Classification | Polyclonal |
| Primary or Secondary | Secondary |
| Test Specificity | Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with mouse IgG, rat IgG, chicken IgY and human IgG is observed. |
| Content And Storage | Store 2 - 8 °C as an undiluted liquid for several weeks. After dilution, do not use for more than one day. -20 °C for up to one year. |
|---|---|
| Target Species | Chicken |
| Host Species | Rabbit |
| Conjugate | HRP |
| Applications | ELISA,Western Blot,Immunohistochemistry,Immunocytochemistry |
| Form | Liquid |
| Isotype | IgY |
| Antigen | Rabbit Anti-Chicken IgY |
| Regulatory Status | RUO |
| Dilution | 1:250-1:3,000 for IHC/ICC., , 1:2,000-1:5,000 for ELISA, Western Blot, color substrates., , 1:5,000-1:10,000 for Western Blot, chemiluminescence. |
| Product Type | Antibody |
| Formulation | Buffer containing 0.01M sodium phosphate, 0.25M sodium chloride, pH 7.6 and 25% glycerol. |
| Classification | Polyclonal |
| Immunogen | Purified chicken IgY. |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody reacts with both heavy and light chains of chicken IgY, minimum cross-reactivity with IgG of mammalian species. |
| Content And Storage | Peroxidase Conjugated Affinity Purified anti-Chicken IgY can be stored vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Target Species | Chicken |
| Host Species | Rabbit |
| Conjugate | HRP |
| Applications | ELISA,Western Blot,Immunohistochemistry |
| Form | Lyophilized |
| Isotype | IgY |
| Antigen | Rabbit Anti-Chicken IgY |
| Regulatory Status | RUO |
| Purification Method | Peroxidase Conjugated Affinity Purified anti-Chicken IgY was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgY coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgY and Chicken Serum. |
| Dilution | Peroxidase Conjugated Affinity Purified anti-Chicken IgY is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:35,000 - 1:150,000, Western Blot 1:3,500 - 1:15,000, Immunohistochemistry 1:1,000 - 1:5,000. (Optimal dilution should be determined by user). |
| Product Type | Antibody |
| Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/mL Bovine Serum Albumin (BSA); 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Classification | Polyclonal |
| Immunogen | Chicken IgY - whole molecule |
| Primary or Secondary | Secondary |
IgG(H+L) Rabbit F(ab')2 anti-Human, Unconjugated, Polyclonal, Southern Biotech™
Rabbit F(ab')2 Polyclonal Secondary Antibody