PCR Cloning Kits
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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli (Supply Center Packaging)
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Promoter | SP6 |
|---|---|
| Content And Storage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
| Antibiotic Resistance Bacterial | Kanamycin (KanR), Zeocin™ (ZeoR) |
| Format | Kit |
| Product Type | PCR Cloning Kit |
| Cloning Method | Blunt TOPO™ |
Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ CloneJET PCR Cloning Kit
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
| Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
|---|---|
| Format | Kit |
| Sample Type | PCR Products |
| Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
| For Use With (Application) | PCR Cloning |
| Vector | pJET1.2/blunt |
| Product Line | CloneJET |
Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli
Efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Promoter | SP6 |
|---|---|
| Content And Storage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
| Format | Kit |
| Product Type | PCR Cloning Kit |
| Cloning Method | Blunt TOPO™ |
| Bacterial or Yeast Strain | TOP10 |
| Cell Type | Chemically Competent |
Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Content And Storage | Box 1: • Topoisomerase I-activated pCR™2.1-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
|---|---|
| Format | Kit |
| Product Type | Cloning Kit |
| Cloning Method | TOPO™-TA |
| Bacterial or Yeast Strain | TOP10 |
| Cell Type | Chemically Competent |
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Promoter | T7, T3 |
|---|---|
| Format | Kit |
| Product Type | PCR Cloning Kit |
| Cloning Method | Blunt TOPO™ |
| Bacterial or Yeast Strain | TOP10 |
| For Use With (Application) | Chromatin Biology |
| Vector | Blunt DNA Cloning Vectors |
| Product Line | One Shot™ |
| Cell Type | Chemically Competent |
Invitrogen™ pENTR™/TEV/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli
Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with TOP10 E. coli (Supply Center Packaging)
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
|---|---|
| Format | Kit |
| Product Type | Cloning Kit |
| Cloning Method | TOPO™-TA |
| Product Line | TOPO™ |
Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Promoter | T7, SP6 |
|---|---|
| Content And Storage | Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
| Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
| Format | Kit |
| Cloning Method | TOPO™-TA |
| Vector | TOPO-TA Vectors |
| Product Line | TOPO™ |
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, without competent cells
Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
| Shipping Condition | Dry Ice |
|---|---|
| Promoter | T7, T3 |
| Content And Storage | Box 1: • Topoisomerase I-activated pCR™4Blunt-TOPO™ vector • Salt solution • dNTPs • Control template • T3, T7, M13 forward, and M13 reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
| Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
| Format | Kit |
| Product Type | PCR Cloning Kit |
| Cloning Method | Blunt TOPO™ |
| Vector | TOPO Cloning Vectors |
| Product Line | TOPO™ |
Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli (Supply Center Packaging)
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More