Invitrogen™ RNase T1 (1 U/μL)

Description

Includes

RNase T1, 10X RNA Structure Buffer, 1X Alkaline Hydrolysis Buffer, Yeast RNA, Precipitation/Inactivation Buffer, and Gel Loading Buffer II

Invitrogen™ RNase T1, RNA-Grade cleaves 3' of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites.

Invitrogen™ RNase T1, RNA-Grade cleaves 3′ of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites. Supplied in one tube of 200μL (1U/μL). RNase T1 can be used to perform boundary experiments to define the minimal RNA sequence required for selectable activities such as protein binding or catalysis. In addition to applications for RNA structural analysis, RNA-Grade ribonucleases can be used to map protein binding sites on RNAs by comparing cleavage patterns in the presence and absence of an RNA binding protein.

• 100 Units is the amount of enzyme that yields an increase in absorption at 260 nm of 0.01428 units/min. at room temperature using 60 μg/mL yeast total RNA as a substrate.
• RNase A (Cat. No. AM2274) cleaves 3′ of single-stranded C and U residues. Combinations of the single- and double-stranded specific ribonucleases can provide rapid analysis of the physical structure of an interesting RNA.

Order Info

Shipping Condition: Dry ice

Specifications

• Concentration: 1 U/μL
• Content And Storage: RNase T1, 10X RNA Structure Buffer, 1X Alkaline Hydrolysis Buffer, Yeast RNA, Precipitation/Inactivation Buffer, and Gel Loading Buffer II
  
  Store all components at –20°C.
• Format: Tube
• Enzyme: RNase
• Fidelity (vs. Taq): 10X
• For Use With (Application): Real Time PCR (qPCR)
• No. of Reactions: 200
• Product Line: Ambion™
• Product Type: Rnase T1 PCR Kit
• Quantity: 200 μL
• Shipping Condition: Dry Ice
• Source: RNA
• Form: Solution

For Research Use Only. Not for use in diagnostic procedures.