Transfection Reagents
Dojindo Molecular Technologies Inc HilyMax (1ML x 1) is a high transfection efficiency reagent, and is the best reagent for cell signaling research.
HilyMax is a gene transfection reagent that forms a liposome to be used for highly efficient gene transfection to a wide variety of cells. HilyMax readily interacts with DNA because cationic liposomes (+) and anionic DNA (-) spontaneously form DNA liposome complexes. The overall charge of DNA-HilyMax complex is positive, such that the DNA-HilyMax complex is electrostatically bound on an anionic cell surface and introduces DNA into the cell by endocytosis. Since the serum in the growth medium does not interfere with the transfection using HilyMax, no exchange of the medium during the transfection is required. HilyMax does not contain biological components that might interfere with the transfection.
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT DUO TRANSRGNT .75ML
DharmaFECT Duo Transfection Reagent 0.75mL
Enzo Life Sciences Nupherin™ Transfection Reagent (3 mg)
Nupherin™ dramatically enhances the performance of commercially available cationic lipids to allow high-efficiency lipofection (10-40%) of neuronal, ganglion and other difficult to transfect cells. Efficient lipofection is limited by the ability of transfected DNA to enter the nucleus – a particular obstacle in nondividing or slow dividing cells like neurons. Nupherin, a peptide based reagent that combines a non-classical nuclear localization sequence (M9) with a nucleotide binding domain, helps to transport DNA into the transfected cell's nucleus. Nupherin has been demonstrated to provide high efficiency lipofection of primary rat hippocampal neurons, chicken embryonic retinal ganglian cells, rat pheochromocytoma (PC12) cells and other recalcitrant cells. Fully compatible with cells grown on glass coverslips, in plastic plates, or in tissue-culture flasks. Formulation: Liquid. Sterile solution. Long Term Storage: -80°C.
Sigma Aldrich Fine Chemicals Biosciences Hexadimethrine bromide | 28728-55-4 | MFCD00133397 | 100 g
Hexadimethrine bromide | Purity: ≥94% | Mol Wt: 1122.61 | 28728-55-4 | MFCD00133397 | 100 g
Lonza Walkersville Cell Line Nucleofector Kit C
The Cell Line Nucleofector Kit C is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. This kit has been determined to be optimal for transfecting following cell types:A2058CCRF-CEMMDA-MB-453MEG-01MG-63NCI-H1299 [H1299]NS0 (ECACC)SK-BR-3T2U266B1U-937The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution C0.5 mL Supplement 230 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Mouse Embryonic Fibroblast Nucleofector Kit 2
The Nucleofector 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. The Mouse Embryonic Fibroblast Nucleofector Kit 2 is one of our two kits suited for efficient transfectiion of primary mouse embyonic fibroblasts (MEF). Due to significant diversity between different MEF clones, we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit to determine which of the 2 kits is optimal for a specific MEF clone.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells -Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Mouse Embryonic Fibroblasts0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Smooth Muscle Cells
In addition to various cell type-specific kits for primary smooth muscle cells, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian smooth muscle cell type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for smooth muscle cells. Benefits:Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support TeamTransfection efficiencies up to 95%Viabilities up to 96%Applications:Kit suited for smooth muscle cells from different mammalian species and various organs Already tested for porcine vascular smooth muscle cells and coronary artery smmoth muscle cells (CASMC)
Lonza Walkersville Cell Line Nucleofector Kit L
The Cell Line Nucleofector Kit L is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device.This kit has been determined to be optimal for transfecting following cell types:3T3-L1 adA-10BHK-21BxPC-3DU145EL4FDC-P1GH3 H9c2 (2-1)HeLa S3IMR-32KG-1aL-428 (DSMZ)MDCKMDCK II (ECACC)MOLT-4MV-4-11NTERA-2 cl.D1WEHI-231The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette format
Kyfora Bio Transporter 5 Transfection Reagent 50ML
Kyfora Bio's Transporter 5 is a high-quality, ready-to-use transfection reagent derived from our popular PEI MAX (Polyethyleneimine MAX). This versatile reagent is suitable for both stable and transient transfections across a variety of cell lines, including HEK293 and CHO cells. Transporter 5 consistently delivers high yields of viral vectors (AAV and LV), recombinant proteins, and monoclonal antibodies, all while maintaining optimal cell viability. Its scalability and compatibility with a range of cell culture media make it an excellent choice among available transfection reagents on the market.
Kyfora Bio Transporter 5 Transfection Reagent 5ML
Kyfora Bio's Transporter 5 is a high-quality, ready-to-use transfection reagent derived from our popular PEI MAX (Polyethyleneimine MAX). This versatile reagent is suitable for both stable and transient transfections across a variety of cell lines, including HEK293 and CHO cells. Transporter 5 consistently delivers high yields of viral vectors (AAV and LV), recombinant proteins, and monoclonal antibodies, all while maintaining optimal cell viability. Its scalability and compatibility with a range of cell culture media make it an excellent choice among available transfection reagents on the market.
Takara Bio Xfect™ RNA Transfection Reagent | 1.2mL
Xfect RNA Transfection Reagent is a complete system for highly efficient transfection of mammalian cells with all types of RNA, including messenger RNA (mRNA), small interfering RNA (siRNA), and single guide RNA (sgRNA).The protocol is simple, and transfections can be carried out entirely in the presence of serum. Xfect RNA Transfection Reagent creates biodegradable nanoparticles when combined with RNA, resulting in very low cytotoxicity.