Transcription and Translation Reagents
Trilink Bio Technologies Inc ARCA, 1 umole
A key step in cellular mRNA processing is the addition of a 5’ cap structure, which is a 5'-5' triphosphate linkage between the 5' end of the RNA and a guanosine nucleotide. The cap is methylated enzymatically at the N-7 position of the guanosine to form mature mCAP. When preparing synthetic mRNA, the cap is often added prior to use in order to stabilize the mRNA and significantly enhance translation. Using a 4:1 mixture of a cap analog to GTP in transcription reactions will cap 80% of the resulting mRNAs. If mCAP (pre-methylated CAP) is used, only half of the asymmetrical mCAP will insert in the functional orientation. ARCA can only insert in the correct orientation, resulting in mRNAs that are translated twice as efficiently.
BIOSEARCH TECHNOLOGIES INC (Lucigen) MMLV Reverse Transcriptase 1St-Strand cDNA Synthesis Kit, 50 RxnsOptimised, complete system for production of full-length cDNA from low amounts of total RNA.
Synthesize full-length cDNA (15 kb)Generate first-strand cDNA from picogram amounts of total RNASelect oligo(dT) or random primers - both includedKit contains MMLV Reverse Transcriptase, 10X Reaction Buffer, RiboGuard RNase Inhibitor, dNTP PreMix, DTT, Oligo(dT)21 Primer, Random 9-mer Primers, RNase-free Water.
Roche Diagnostics TRANSCRIPTOR 1ST STRD CDNA KIT
Transcriptor First Strand cDNA Synthesis Kit; Contains a recombinant reverse transcriptase for the robust transcription of RNA fragments up to 14 kb
Carnabio Usa Inc PEK(EIF2AK3)/10UG/CYTOPLASMIC
PEK(EIF2AK3), Cytoplasmic domain, Wild type, Amino Acid 536-1116, NP_004827.3, Expressed in E. coli; 10microg
New England Biolabs, Inc. HiScribe T7 mRNA Kit with CleanCap Reagent AG
The HiScribe T7 mRNA Kit with CleanCap Reagent AG utilizes an optimized RNA synthesis formulation and trinucleotide cap analog technology for co-transcriptionally capping mRNAs that contain a natural Cap-1 structure in a single simplified reaction without compromising RNA yield. By using a DNA template with a T7 promoter sequence followed by an AG initiation sequence and an encoded poly(A) tail mRNAs can be transcribed with a 5-m7G Cap-1 structure that is polyadenylated translationally competent and able to evade the cellular innate immune response. The kit is formatted with individual vials of NTPs and CleanCap Reagent AG to allow for partial or complete substitution of modified NTPs with a total kit yield of 1.8 mg of mRNA. Cap-1 mRNA synthesized from this kit is suitable for many applications including transfections microinjections in vitro translation preclinical mRNA therapeutic mRNA studies as well as RNA structure and function analysis.
Apexbio Technology LLC CO-TRA ARCA 5MCTP ΨUTP T7 25R
CO-TRA ARCA 5MCTP UTP T7 25R APEXBIO TECHNOLOGIES
Invitrogen™ Retic Lysate IVT™ Kit
Efficiently translates high molecular weight proteins and both capped and uncapped mRNAs
R&D Systems™ Human/Mouse/Rat Neural Lineage Functional Identification Kit
To verify the multipotency of neural progenitor cells (NPCs) by in vitro functional differentiation
BD Mouse Pluripotent Stem Cell Transcription Factor Analysis Kit
For measuring expression of the stem cell master regulators'Nanog, Oct3/4, and Sox2'in heterogenous stem cell populations
R&D Systems™ Rat Mesenchymal Stem Cell Functional Identification Kit
To verify multipotency of rat mesenchymal stem cells by in vitro functional differentiation
Cayman Chemical Nuclear Extraction Kit, 78782-99-7, 1 ea
Preparation of nuclear extracts is the first step in examining transcription factor activity. Cayman’s Nuclear Extraction is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in Cayman’s Transcription Factor Assay kits. The proteins isolated using this kit can also be used in electrophoretic mobility shift assays (EMSA) and western blotting applications.