Enzymes and Inhibitors
Cayman Chemical TR-FRET asy Buffer 210X 2mL
Coriolis consumables ensure optimal collection efficiency for the Coriolis Air Sampler.
Sino Biological Peptide Substrates: JAK3tide
The JAK3tide synthetic peptide [GGEEEEYFELVKKKK] is routinely evaluated as a substrate for JAK3 and JAK2.
AdipoGen Fluorescein diacetate (10 g)
Fluorescein diacetate (10 g), AdipoGen Life Sciences. Chemical. CAS: 596-09-8. Formula: C24H16O7. MW: 416.38. Synthetic. FDA is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the diacetate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. FDA may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green.FDA is used in studies on intracellular interactions and membrane permeability, for flow cytometry and as esterase substrate. Spectral data: λex=494nm, λem=521nm.
Medchemexpress LLC D-luciferin sodium | 103404-75-7 | 99.9% | 302.30 g·mol⁻1 | C11H7N2NaO3S2 | 10MG
D-Luciferin sodium is the sodium salt of D-luciferin, the natural substrate for luciferase enzymes. It is supplied as a high-purity reagent for sensitive bioluminescent detection in applications such as reporter gene assays, cell viability testing, and in vivo imaging, providing a reliable luminescent signal when used with appropriate luciferase systems.
- High purity suitable for sensitive bioluminescent assays.
- Water-soluble sodium salt formulation for easy preparation.
- Compatible with in vitro and in vivo luciferase-based imaging.
- Available in multiple pack sizes for small- and large-scale experiments.
- Stable when stored according to supplier instructions (refer to SDS).
Ubpbio K63-linked polyubiquitin chain mix containing 2-12 human ubiquitin, enzymatically synthesized
Ub chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.
Abcam 5(6)-CFDA N-succinimidyl ester (CFSE), Fluorogenic esterase substrate, 50MG
MW 557.46 g/mol, Purity >90%. Cell-permeable, fluorogenic, non-selective esterase substrate. Hydrolyzes to hydrophilic fluorescent 5(6)-CFDA (ab145278) and 5(6)-FAM (ab145342) derivatives intracellularly. Partitions with high fidelity between daughter cells for up to eight generational divisions. Tracks cell proliferation.
The product is subject to the following: Abcam Restricted Use Statement
Sino Biological Peptide Substrates: MLC Peptide
The MLC Peptide sequence (AKRPQRATSNVFS) represents the phosphate-accepting domain of human MYL9 (amino acids 12-23) and can be used as a substrate for PHKG1, PHKG2 and DAPK2.
Cayman Chemical EnTRopeptIdase FluorogenIc
A substrate for enteropeptidase; enteropeptidase recognizes amino acid sequence DDDDK and cleaves after the lysine residue, releasing AFC, which displays ex/em spectra of 400/505 nm
Sino Biological Peptide Substrates: CSKtide
The synthetic peptide CSKtide (KKKEEIYFFFG-NH2) contains a tyrosine protein kinase phosphorylation site and can be used for CSK and TNK1 assay.
Medchemexpress LLC Ac-lys-AMC (Hexanamide) | 156661-42-6 | 98.9% | 1 ML
Ac-Lys-AMC (Hexanamide), also known as MAL, is a fluorescent substrate utilized for assaying histone deacetylases (HDACs). It is intended strictly for research purposes and is not sold to patients.
- Fluorescent substrate.
- Designed for the assay of histone deacetylase HDACs.
- Purity of 98.93%.
- Appears as a white to off-white solid.
- Soluble in DMSO at 100 mg/mL in vitro, requiring sonication.
- In vivo protocols offer clear solutions with solubility of ≥ 2.5 mg/mL in various co-solvent systems.
- Powder stable for 3 years at -20°C and 2 years at 4°C.
- In solvent, stable for 6 months at -80°C and 1 month at -20°C.
Medchemexpress LLC D-luciferin | 2591-17-5 | MFCD00042929 | >99.0% | 280.32 g/mol | C11H8N2O3S2 | 5MG
D-Luciferin is the natural substrate for luciferase enzymes used in bioluminescent assays and imaging. It produces visible light upon enzymatic oxidation in the presence of ATP, enabling sensitive detection in cell-based and in vivo studies.
- High purity (≥99.0%) suitable for analytical and biological applications.
- Effective luciferase substrate for bioluminescent assays and imaging.
- Soluble in DMSO (125 mg/mL) and sparingly soluble in water unless pH is adjusted.
- Stable when stored protected from light at 4°C; solutions stable at -80°C for extended storage.
- Molecular formula C11H8N2O3S2 and molecular weight 280.32 g/mol.
Sino Biological Peptide Substrates: LKBtide
The synthetic peptide LKBtide (LSNLYHQGKFLQTFCGSPLY-RRRC) is derived from human NUAK2 (amino acid 196-215) and can be phosphorylated by protein Serine/Threonine kinase 11 (STK11), also known as LKB1.
Medchemexpress LLC Receptor tyrosine-protein kinase ErbB-2 (HER2/CD340), rhesus macaque, recombinant, His-tagged | >95.0% | 50 UG
Recombinant HER2/CD340 protein from rhesus macaque (extracellular residues Q24-T652) is produced in HEK293 cells with a C-terminal His tag and supplied lyophilized for use in antibody characterization, ligand binding assays, and signaling studies.
- Mammalian expression preserves native folding and glycosylation.
- C-terminal His tag enables affinity purification and detection.
- Purity greater than 95% as determined by reducing SDS-PAGE.
- Lyophilized from PBS, pH 7.4, for improved stability and storage.
- Available in multiple sizes, including a 50 μg vial.
Bachem Z-Lys-SBzl · HCl
Z-Lys-SBzl · HCl, 69861-89-8, C21H26N2O3S · HCl, Mr 422.98, 50mg. Substrate for a sensitive colorimetric assay for trypsin-like enzymes, e.g. kallikreins, thrombin, plasmin, trypsin, and enteropeptidase. A sensitive, coupled assay for plasminogen activators (urokinase and tPA) using this substrate has been described. CAS-Number (net) 69861-90-1.
Sino Biological Peptide Substrates: AMARA Peptide
The AMARA substrate peptide sequence (AMARAASAAALARRR) is routinely evaluated as a substrate for SIK and AMPK.