Modifying Enzymes
New England Biolabs, Inc. Thermostable FEN1 – 1600 units
Thermostable Flap Endonuclease 1, FEN1, catalyzes the cleavage of 5 DNA flaps from branched double stranded DNA substrates, creating a 5' phosphate terminus. FEN1 products can be ligated by DNA ligase to create double stranded DNA. In vivo, FEN1 is an essential component of the Okazaki fragment maturation pathway, and also plays a role in base excision repair.
Bioworld alpha-Chymotrypsin, 500 mg
Specification:Grade: USP gradeForm: White crystalline powderChymotrypsin acitvity (dry basis): 1000 U/mgTrypsin: 1%Residue after ignition: 2.5%Loss on drying: 5%Solubility (water, 10,000 U/ml): Pass
GENOVIS INC FABRICATOR 8 STRIP WELL
FabRICATOR, 8×100 Units Lyophilized FabRICATOR (IdeS) for digestion of 8 x 100 μg of antibody. Enzyme is reconstituted by addition of water. FabRICATOR (IdesS) is a unique protease that cleaves IgGs and Fc-fusions at one single amino acid position in the lower hinge region generating Fc and F(ab')2 fragments.
Bioworld alpha-Chymotrypsin, 1 g
Specification:Grade: USP gradeForm: White crystalline powderChymotrypsin acitvity (dry basis): 1000 U/mgTrypsin: 1%Residue after ignition: 2.5%Loss on drying: 5%Solubility (water, 10,000 U/ml): Pass
WORTHINGTON BIOCHEMICAL CORPORATION TRYPSIN PURIFIED SEQ GRADE II
NC2210228 TRYPSIN PURIFIED SEQ GRADE II
AG Scientific Inc Cholesterol Esterase 100 U
Cholesterol esterase catalyzes the hydrolysis of sterol esters into their component sterols and fatty acids. The enzyme is found primarily in the pancreas, but has been detected in other tissues as well. Bile salts, such as cholate and its conjugates, are required to stabilize the enzyme in its native polymeric form and to protect it from proteolytic hydrolysis in the intestine.Cholesterol esterase is used for determination of cholesterol in serum and plasma, with cholesterol oxidase or peroxidase and synthesis of optically active alcohols and carboxylic acids. Its activators are Bile salts, Cholate, Glycocholate, Taurocholate and inhibitors are PMSF and p-chloromercuribenzoate, Diisopropyl fluorophosphate, Hg2+, Ag+, and ionic detergents, Aryl carbamates.
ACROBiosystems PNGase F 500U/ul
PNGase F (500U/ul) is expressed from Elizabethkingia miricola E. coli.
Worthington Biochemical Corporation DEOXYRIBONUCLEASE I RN 100UN
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied as a solution at approximately 2 Kunitz units per microliter (approximately 1 mg/ml) containing 50% glycerol and 1 mM calcium chloride. 9003-98-9
Worthington Biochemical Corporation STEMXYME 1 COLLAGENAS 50MG
A specialized combination of Animal Origin-Free Clostridium histolyticum collagenase (Code: CLSAFB) and animal origin free Bacillus polymyxa neutral protease with a minimum of 250 CLS units and 1,000 caseinase units per mg dry weight. Designed for stem cell and other primary cell isolations and bioprocessing applications where introduction of potential animal derived pathogens must be prevented. 2593-92-3
Worthington Biochemical Corporation PAPAIN LYOPHILIZED 1GM
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To insure full activity, the enzyme should be incubated in a solution containing 1.1 mM EDTA, 0.067mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use. 9001-73-4
Worthington Biochemical Corporation ELASTASE LYOPHILIZED 1GM
Two times crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior to use. Suitable for the isolation of Type II lung cells. 2594-83-7
New England Biolabs, Inc. Template Switching RT Enzyme Mix – 100 reactions
The reverse transcriptase (RT) in the Template Switching RT Enzyme Mix adds a few non-templated nucleotides after it reaches the 5' end of the RNA template. These non-templated nucleotides can anneal to a template switching oligo (TSO) with a known sequence, prompting the reverse transcriptase to switch template from RNA to the TSO. The resulting cDNA contains a known sequence (complementary to the sequence of the TSO) attached to the 3' end. This feature can be utilized in a variety of downstream applications, such as cDNA amplification, 5' RACE (rapid amplification of cDNA ends), and 2nd strand cDNA synthesis.