Modifying Enzymes
Athens Research And Technology Inc ELASTASE HU NEUTROPHIL 1MG
Elastase, Human Neutrophil: 1 MG; Salt-Free lyophilized solid.; MW 29,500; EC = 0.985; Activity: 20-22 units per mg protein; Purity: > =95% by SDS-PAGE. This native protein is prepared and purified from whole blood in the Athens Research & Technology laboratory. Molecular Biology Reagent
Worthington Biochemical Corporation Miccrococal Nuclease, 45ku
Nuclease, Micrococcal (S7); Micrococcal nuclease (MN) catalyzes cleavage of both DNA and RNA to yield 3'-nucleotides; MN is the extracellular nuclease of Staphylococcus aureus; M.W. 16807; Optimum 9.2 pH; Stored: 2 deg. to 8 deg.C
Athens Research And Technology Inc CERULOPLASMIN HU 1MG
Ceruloplasmin, Human Plasma: 1 MG; Lyophilized from 50 mM potassium phosphate, pH 6.8, 100 mM KCl and 20 mM E-amino caproic acid + 5 mM EDTA. MW 132,000; EC=1.5; Purity: > =95% by SDS-PAGE. Prepared from human plasma in the Athens Research & Tech lab.
Ec-Oxyrase Enzyme System, Sterile, 50Ml Bottle 1/Pk
Oxyrase Unit defined as amount of activity that reduces dissolved oxygen @ 37°C & pH 8.4 in a phosphate buffer with lactate substrate @ rate of 1% per second. 0.3 unit of EC-Oxyrase reduces dissolved oxygen @ saturation to levels measured in parts per billion (ppb) in about 4 minutes. Active over wide temperature range. Operates over wide pH range 6.8 to 9.4.
Ubpbio N-terminally 6xHis-tagged recombinant human UbC13/UEV1A (UbE2N), a ubiquitin conjugating enzyme catalyzing formation of K63 polyubiquitin chains
UbE2N (Ubc13/UEV1A) is a complex that acts as an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates. Uev1a must interact with Ubc13 because it lacks the active site that other E2s posses. Ube2N has been known to catalyze K63 – linked Ub chains which aides in activation of kinases in NF – kappaB signaling pathway, amongst other tasks.
New England Biolabs, Inc. Apyrase – 10 units
Apyrase (recombinant, E. coli) is a highly active ATP-diphosphohydrolase that catalyses the sequential hydrolysis of ATP to ADP and ADP to AMP releasing inorganic phosphate. It is a recombinant version of one of several isoforms of apyrase. It can also hydrolyse 5' tri- and diphosphate ribonucleosides and deoxyribonucleosides to their respective 5' monophosphates. Apyrase can catalyse the conversion of 5' triphosphorylated RNA to 5' monophosphorylated RNA.
New England Biolabs, Inc. Apyrase – 50 units
Apyrase (recombinant, E. coli) is a highly active ATP-diphosphohydrolase that catalyses the sequential hydrolysis of ATP to ADP and ADP to AMP releasing inorganic phosphate. It is a recombinant version of one of several isoforms of apyrase. It can also hydrolyse 5' tri- and diphosphate ribonucleosides and deoxyribonucleosides to their respective 5' monophosphates. Apyrase can catalyse the conversion of 5' triphosphorylated RNA to 5' monophosphorylated RNA.
American Research Products Inc Mouse phosphoenolpyruVate carb
Mouse phosphoenolpyruVate carboXykinase PCK ELISA Kit from CUSABIO Detection Range 1 56 pg/mL-100 pg/mL Sensitivity 0 39 pg/mL Sample serum plasma tissue homogenates Method Sandwich
STEMCELL Technologies ArciTect™ T7 Endonuclease I Kit, Size: 125 Reactions
ArciTect™ T7 Endonuclease I is the preferred enzyme for detecting genome editing such as insertions or deletions (INDELs) generated by CRISPR-Cas9. ArciTect™ T7 Endonuclease I Kit is comprised of ArciTect™ T7 Endonuclease I and ArciTect™ T7 Endonuclease I Buffer (10X), which have been tested and validated for use with the ArciTect™ CRISPR-Cas9 genome editing system. ArciTect™ T7 Endonuclease I recognizes and cleaves mismatched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA, and, less efficiently, nicked double-stranded DNA. Since the cleavage efficiency is proportional to the number of INDELs created at a specific DNA target, ArciTect™ T7 Endonuclease I Kit is used to estimate gene-editing efficiency in a rapid and cost-effective manner.
STEMCELL Technologies ArciTect™ T7 Endonuclease I Kit, Size: 25 Reactions
ArciTect™ T7 Endonuclease I is the preferred enzyme for detecting genome editing such as insertions or deletions (INDELs) generated by CRISPR-Cas9. ArciTect™ T7 Endonuclease I Kit is comprised of ArciTect™ T7 Endonuclease I and ArciTect™ T7 Endonuclease I Buffer (10X), which have been tested and validated for use with the ArciTect™ CRISPR-Cas9 genome editing system. ArciTect™ T7 Endonuclease I recognizes and cleaves mismatched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA, and, less efficiently, nicked double-stranded DNA. Since the cleavage efficiency is proportional to the number of INDELs created at a specific DNA target, ArciTect™ T7 Endonuclease I Kit is used to estimate gene-editing efficiency in a rapid and cost-effective manner.
American Research Products Inc ASPARTATE TRANSAMINASE AST KIT
5000265273 ASPARTATE TRANSAMINASE AST KIT
Enzyme Research Laboratories Inc HUMAN FACTOR ALPHA XIIA 0.5MG
Human Factor alpha -XIIa is a serine protease responsible for the activation of Factor XI to XIa in the contact activation system. The protein purity is determined by SDS-PAGE and activity is determined via clotting assay.