Modifying Enzymes
INTACT GENOMICS INC Exonuclease III 25000 Units (100 units/µl)
Exonuclease III (Exo III) is an exodeoxyribonuclease that digests one strand of duplex DNA from a blunt end, 5′ overhang or nick. The enzyme acts in a 3′→5′ direction producing stretches of single-stranded DNA (1, 2). Exo III is not active on 3′ overhangs of 4 or more bases in length and ssDNA. The enzyme has also RNase H, 3′-DNA phosphatase, and apurinic DNA endonuclease activities. Exo III digestions proceed at a uniform rate yielding predictable and reproducible results. However, the observed rate of nucleotide excision can vary depending on the individual characteristics of the reaction mix such as reaction temperature, ionic strength, template base content, template helical structure, and enzyme-to-DNA ratios.Applications• 3′ → 5′ exonuclease• Production of unidirectional nested deletions• Production of intermediates for site-directed mutagenesis• Preparation of strand-specific probes• Preparation of single-stranded substrates for dideoxy sequencing
Cayman Chemical Oleoyl SerotonIn-d17 1mg
An internal standard for the quantification of oleoyl serotonin by GC- or LC-MS
Medchemexpress LLC Catalase, aspergillus niger | 9001-05-2 | 500 MG
Catalase, Aspergillus niger is a key enzyme involved in the metabolism of H2O2 and reactive oxygen species (ROS), with its expression and localization being significantly altered in tumors. It functions as a free oxygen radical scavenger.
- Produced through fermentation by Aspergillus niger.
- Effective within a pH range of 4.0 to 10.0.
- Effective within a temperature range of 35°C to 40°C.
- Can be dissolved using water or as specified in relevant references.
- The active site contains an iron atom.
- Contains a N-terminal arm with a distal histidine, essential for the catalase reaction.
- Features a β-barrel domain with eight β-barrels arranged antiparallel with six α-helical insertions, forming the hydrophobic core.
- Includes a connection domain containing a tyrosine residue that binds the heme group.
- Has α-helical domains important for NADPH binding.
ABclonal Technology T4 DNA Ligase (High Conc.)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.
New England Biolabs, Inc. Factor Xa Protease – 250 µg
Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-Glu/Asp-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate. The most common secondary site, among those that have been sequenced, is Gly-Arg.
Qiagen, Inc. RNASE A (17 500 U)
Enzyme, RNase A; Qiagen; For RNase digestion during DNA preparation, ready to use, store at room temp. up to 2 yrs, 2.5mL (100mg/mL; 7000U/mL, solution)
Sigma Aldrich Fine Chemicals Biosciences Papain from papaya latex | 9001-73-4 | MFCD00131791 | 25mg
Papain from papaya latex | Mol Wt: 23.4 kDa | 9001-73-4 | MFCD00131791 | 25mg
Sigma Aldrich Fine Chemicals Biosciences Superoxide Dismutase from bovine liver | 9054-89-1 | MFCD00132404 | 3ku
Superoxide Dismutase from bovine liver | Mol Wt: 32.5 kDa | 9054-89-1 | MFCD00132404 | 3ku
Athens Research And Technology Inc KALLIKREIN HU PLASMA 50UG
Kallikrein, Human Plasma: 50 UG; Frozen in 20 mM Tris-HCl, pH 7.8, with 100 mM NaCl; MW 86,000-88,000; EC= 1.06; Activity: > =15 U/MG; Purity: > =95% by SDS-PAGE. Native; Prepared and purified from human plasma. Mol. Biology Reagent, Serine Protease
![Biolegend PerCP/Fire™ 780 anti-human CD4 [SK3]; Isotype: Mouse IgG1, κ; Reactivity: Human; Apps: FC; Size: 100 tests](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502530554.png-250.jpg)
Biolegend PerCP/Fire™ 780 anti-human CD4 [SK3]; Isotype: Mouse IgG1, κ; Reactivity: Human; Apps: FC; Size: 100 tests
PerCP/Fire™ 780 anti-human CD4 [SK3]; Isotype: Mouse IgG1, κ; Reactivity: Human; Apps: FC; Size: 100 tests
Sino Biological Recombinant DNase I (RNase-free) 5000 U
DNase I is an endonuclease that can non-specifically cleave DNA, producing dinucleotide, trinucleotide, and oligonucleotide products with 5 ́ phosphorylated and 3 ́ hydroxylated ends. DNase I acts on single-and double-stranded DNA, chromatin, and RNA:DNA hybrid strands. Certified by highly sensitive fluorescence probe assays, our DNase I (RNase-free) demonstrates virtually undetectable RNase activity-a level of purity that surpasses other commercial sources. Trust in this "Ultra-Pure" standard for the ultimate reliability in your RNase-sensitive workflows.
Biotium CD45 / LCA (Leucocyte Marker)(Bra55), CF594 conjugate, 0.1mg/mL
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Biotium CD45 / LCA (Leucocyte Marker)(Bra55), CF647 conjugate, 0.1mg/mL
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Cayman Chemical N-Oleoyl ValIn 25mg
An endogenous N-acyl amine; antagonist at TRPV3; increases after acute lung injury in mouse lung tissue