Modifying Enzymes
Sigma Aldrich Fine Chemicals Biosciences Apyrase from potato recomb
Apyrase also known as ATP-diphosphohydrolase is expressed in wide variety of plant and animal tissues. The commercially accessible apyrase enzyme is isolated from potato tubers.
Cayman Chemical ANTIBODY KU-0058948 5mg
A PARP1 and PARP2 inhibitor (IC50s = 3.4 and 1.5 nM, respectively); selective for PARP1 over PARP3, PARP4, and tankyrase (IC50s = 40, 1,200, and >10,000 nM, respectively); selectively inhibits the survival of embryonic stem cells lacking Brca1 or Brca2 over embryonic stem cells expressing wild-type Brca1 or Brca2; decreases the survival and induces apoptosis in P39 and MUTZ-3 AML cells at 1 µM; potentiates MS-275-induced cytotoxicity in patient-derived AML cells at 5 nM; reduces survival in PTEN-deficient HCT116 cells but not those expressing wild-type PTEN or HCT116+/neo
New England Biolabs, Inc. EpiMark Methylated DNA Enrichment Kit – 25 reactions
The EpiMark Methylated DNA Enrichment Kit will selectively bind and enrich double-stranded methyl-CpG DNA from fragmented genomic DNA with as little as 5 ng of input DNA. Methylated DNA is isolated from fragmented genomic DNA (5 ng-25 ug) by binding to the methyl-CpG binding domain of human MBD2 protein fused to the Fc tail of human IgG1 (MBD2-Fc), which is coupled to paramagnetic hydrophilic protein A beads (MBC2-Fc/Protein A Magnetic Bead). Two Fc domains can be bound to one site on protein A with high affinity (Kd=10-7). As the Fc fragment is a dimer, four MBD2 domains are exposed to the solvent per molecule of protein A, increasing the relative equilibrium constant 100-fold. This stable complex will selectively bind double-stranded methylated CpG containing DNA. After simple wash steps followed by magnetic capture, the enriched DNA sample is easily eluted in a small volume of nuclease-free water by incubation at 65C.
New England Biolabs, Inc. α2-3,6,8 Neuraminidase - 10000 units
2-3,6,8 Neuraminidase catalyzes the hydrolysis of 2-3, 2-6, and 2-8 linked sialic acid residues from glycoproteins and oligosaccharides.
New England Biolabs, Inc. Lambda Protein Phosphatase – 20000 units
Lambda Protein Phosphatase is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues.
New England Biolabs, Inc. Factor Xa Protease – 250 µg
Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-Glu/Asp-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate. The most common secondary site, among those that have been sequenced, is Gly-Arg.
Cayman Chemical MaLNyl Coenzyme AlIthIM5mg
A CoA derivative used in fatty acid and polyketide synthesis and in the transport of α-ketoglutarate across the mitochondrial membrane; may mediate cancer cell cytotoxicity induced by fatty acid synthase inhibition
New England Biolabs, Inc. Antarctic Phosphatase – 1000 units
Antarctic Phosphatase (AnP) is a heat labile alkaline phosphatase purified from a recombinant source. AnP nonspecifically catalyzes the dephosphorylation of 5' and 3' ends of DNA and RNA phosphomonoesters. Also, AnP Hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). AnP is useful in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents religation of linearized plasmid DNA. The enzyme acts on 5' protruding, 5' recessed, and blunt ends. AnP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. AnP is completely and irreversibly inactivated by heating at 80C for 2 minutes, thereby making removal of AnP prior to ligation or end-labeling unnecessary.
Takara Bio T4 DNA LIGASE 125000U (1000 W
Enzyme; Takara; T4 DNA Ligase; 5 x 25,000U; 350U/uL; Source: Recombinant E. coli strain
New England Biolabs, Inc. Thermostable FEN1 – 1600 units
Thermostable Flap Endonuclease 1, FEN1, catalyzes the cleavage of 5 DNA flaps from branched double stranded DNA substrates, creating a 5' phosphate terminus. FEN1 products can be ligated by DNA ligase to create double stranded DNA. In vivo, FEN1 is an essential component of the Okazaki fragment maturation pathway, and also plays a role in base excision repair.
Enterprise Technology Solutions Avery Index Maker Dividers, 5-Tab, 11 X 8.5, White, 1 Set
ABILITYONE 7530014344198 Skilcraft Find reference or presentation materials quicker with customizable tabs. Labels virtually disappear when applied to tabs. Creating tab labels for one or more divider sets is quick and easy. Take advantage of the efficient, patented Easy Applyâ„¢ label strip that allows you to apply labels to all tabs all at once: simply print labels, align them against tabs, smooth down labels and peel the strip. Regardless of your line of business, Index Maker products help turn your written work and presentations into professional, print shop-quality documents in just a matter of minutes- right at your desk. For use with laser and inkjet printers.