Modifying Enzymes
EPICYPHER INC CUTANA pAG-Tn5 for ChIC/CUT&Tag, 50 rxns
Recombinantly produced in E. coli, CUTANA pAG-Tn5 for ChIC/CUT&Tag is a fusion of Proteins A and G to Transposase Tn5. This construct is useful in performing Cleavage Under Targets and Tagmentation (CUT&Tag). The active dimer of Transposase Tn5 is charged with Illumina adapters and ready to be used immediately in CUT&Tag. CUTANA pAG-Tn5 does not contain an epitope tag, which makes it compatible with tag-mediated CUT&Tag.
Takara Bio CRYONASE COLD-ACTIVE NUCLEASE
Enzyme; Cryonase Cold-active Nuclease; Takara; 10,000U (20 U/uLx500 uL); Source: Purified from E. coli; Purity: Protease activity is less than detection limit; Storage: -20 deg.C
Takara Bio TAQ HOT START PREMIX
PCR Reagents; Takara; Hot-Start Taq DNA Polymerase; 100rxns; reduces background, increased specificity, permits room-temperature reaction assembly
New England Biolabs, Inc. Vaccinia Capping System – 400 units
Vaccinia Capping System provides the necessary components to add 7-methylguanylate cap structures (Cap 0) to the 5' end of RNA. In eukaryotes, these terminal cap structures are involved in stabilization, transport, and translation of mRNAs. Enzymatic production of capped RNA is an easy way to improve the stability and translational competence of RNA used for in vitro translation, transfection, and microinjection. Alternatively, use of labeled GTP in a reaction provides a convenient way to label any RNA containing a 5' terminal triphosphate. Vaccinia capping enzyme is composed of two subunits (D1 and D12). The D1 subunit carries three enzymatic activities (RNA triphosphatase, guanylyltransferase and guanine methyltransferase); all necessary for addition of a complete Cap 0 structure, m7Gppp5'N to 5' triphosphate RNA. In vitro transcripts can be capped in less than one hour in the presence of the capping enzyme, reaction buffer, GTP, and the methyl donor, SAM.
LGC GENOMICS LLC (Lucigen) NxGen M-MuLV Reverse Transcriptase, 50,000 UOutstanding cDNA synthesis.
Supplied at 200,000 U/mL in 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% NP-40 Alternative, 50% glycerol, pH 7.6 @ 25 C. Also provided is 10X M-MuLV RT Buffer which in 1X form is composed of 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25 C. 250,000 U is supplied as 5 50,000 U.
LGC GENOMICS LLC (Lucigen) Exonuclease III, 25,000 U @ 200 U/LDigest duplex DNA in a 3 to 5 direction from a nick, blunt end, or 3-recessed end, producing stretches of ssDNA.
Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' endsProtect protruding 3' ends because this exonuclease will not start digestion at protruding 3' overhangsThis product includes both Exonuclease III enzyme and a 10X reaction buffer.
United States Biological Corporation NUCLEASE P-1 100 UNITS
Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase) from Penicillium citrinum, a zinc dependent glyco-enzyme consisting of 270 amino acid residues, hydrolyzes both 3'-5'-phosphodiester bonds in RNA and heat denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity. Nuclease P1 is capable of hydrolyzing single stranded DNA and RNA completely to the level of mononucleoside 5-monophosphates. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mM of sodium chloride at pH 6.0. Activity: > 300U/mg protein using RNA substrate Unit Definition: One unit of nuclease activity is defined as the amount of enzyme that produces 1umole of acid soluble nucleotides from RNA based on E = 10,600 for RNA hydrolysates per minute at 37 �C, pH 5.3.
Crescent Chemical Co Inc CATALASE FROM BOVINE LIVER
Catalase from bovine liver ca. 11 000 U/mg lyophil. salt-free Homogeneous in SDS-PAGE. Ca. 40 000 U/mg protein. Size - 1G Storage Conditions - -15 °C TO -25 °C Catalog Number - 26910.02 CAS 2593710
Cosmo Bio Usa Inc L GLUTAMATE OXIDASE 25 RECOMB
Recombinant L-Glutamate Oxidase (EC 1.4.3.11) produced in E. Coli. Lyophilized. 25 units/vial. Does not react with glutamine, aspartate, D-glutamate or 16 other L-amino acids.
Sigma Aldrich Fine Chemicals Biosciences LIPASE FROM CANDIDA RUGOSA 10G
NC3450504 LIPASE FROM CANDIDA RUGOSA 10G