The AccuBlue Broad Range RNA Quantitation Kit boasts the widest linear range (5–1000 ng RNA) of available RNA quantitation kits, while providing exceptional accuracy, sensitivity, and high RNA selectivity. A mammalian RNA standard is included for accurate quantitation of mammalian cell RNA. The assay is linear between 5 and 1000 ng of RNA per 200 uL assay well, which spans both Qubit HS and Qubit BR ranges. Unlike absorbance-based measurements, RNA Broad Range Dye is selective for RNA over double-stranded DNA, and can tolerate an equimolar amount of dsDNA in the sample without significant effect on RNA quantitation. The AccuBlue Broad Range RNA Quantitation assay has an excitation/emission of 650/670 nm (when bound to RNA) and is suitable for fluorescence microplate readers and for the Qubit fluorometer. This kit has sufficient reagent for 1000 assays.