Molecular Biology Reagents and Kits
IBA LifeSciences StrGate Acptr Vctr pCSG-IBA105
pCSG-IBA105 is a large expression vector with universal features for transient expression of target proteins with an N-terminal Twin-Strep-tag as well as for generation of stable mammalian cell lines. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells and ColE1 origin for a high plasmid copy number. Extrachromosomal replication in mammalian cells could occur either by origin of replication from Epstein-Barr Virus (oriP) or by SV40 ori. For the former the vector provides the EBNA-1 gene and for the latter the cell line has to be latently infected with SV40 or express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). Stable cell lines can be selected by the neomycin resistance gene (NeoR). In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.
IBA LifeSciences StrGate Acptr Vctr pASG-IBA145
The pASG-IBA145 vector is designed for expression of recombinant proteins with an N-terminal Twin-Strep-tag and C-terminal His-tag in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.
Creative Diagnostics Pseudotyped Luciferase rSARS-CoV-2 Spike
Pseudotyped Luciferase rSARS-CoV-2 Spike is a replication-restricted, recombinant pseudotyped lentiviral particles containing SARS-CoV-2 spike protein. Because the infectivity of Pseudotyped Luciferase rSARS-CoV-2 is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices.
Revvity Health Sciences Inc HTRF Human Total EGFR L858R Detection Kit, Control Lysate
HTRF Human Total EGFR L858R Detection Kit, Control Lysate
IBA LifeSciences pASK-IBA3C
pASK-IBA3C is a classic cloning vector devised for E. coli expression of recombinant proteins with the Strep-tagII fused to the C-terminus. It carries the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI), and the inducible tetracycline promoter/operator for the regulated expression of proteins.The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. The recombinant protein will be localized in the cytoplasm.
IBA LifeSciences pASK-IBA4C
pASK-IBA4C is a classic cloning vector devised for E. coli expression of recombinant proteins with the Strep-tagII fused to the N-terminus. It carries the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI), the inducible tetracycline promoter/operator for the regulated expression of proteins, and the ompA signal for periplasmic secretion of the recombinant protein.The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off.
Biochain Institute Inc CancerSeq Paraffin Tissue Tumor Slides: Skin Melanoma, 5 Slides/pk
To generate our CancerSeq line of tissues, genomic DNA was extracted from the FFPE tissues and validated by targeted NGS with Illumina's TruSeq Amplicon - Cancer Panel (TSACP), which includes 48 cancer-related genes. Our prescreened tissues come with detailed information regarding donor and cancer type, as well as NGS information such as chromosomal position, variant type (SNP or indel), alternate allele variations, quality score, depth of coverage, allele type, transcript ID and more. We offer the prescreened tissue as mounted sections or whole block. Please inquire for other options, such as curls or extracted nucleic acids.
Biochain Institute Inc CancerSeq Paraffin Tissue Tumor Block: Skin Melanoma, 1 Block/pk
To generate our CancerSeq line of tissues, genomic DNA was extracted from the FFPE tissues and validated by targeted NGS with Illumina's TruSeq Amplicon - Cancer Panel (TSACP), which includes 48 cancer-related genes. Our prescreened tissues come with detailed information regarding donor and cancer type, as well as NGS information such as chromosomal position, variant type (SNP or indel), alternate allele variations, quality score, depth of coverage, allele type, transcript ID and more. We offer the prescreened tissue as mounted sections or whole block. Please inquire for other options, such as curls or extracted nucleic acids.
Biochain Institute Inc CancerSeq Plus Paraffin Tissue Tumor Sections: Stomach, 5 Curls/pk
To generate our CancerSeq line of tissues, genomic DNA was extracted from the FFPE tissues and validated by targeted NGS with Illumina's TruSeq Amplicon - Cancer Panel (TSACP), which includes 48 cancer-related genes. Our prescreened tissues come with detailed information regarding donor and cancer type, as well as NGS information such as chromosomal position, variant type (SNP or indel), alternate allele variations, quality score, depth of coverage, allele type, transcript ID and more. We offer the prescreened tissue as mounted sections or whole block. Please inquire for other options, such as curls or extracted nucleic acids.
Macherey-Nagel NucleoSpin RNA Clean-up, 250 preps
NucleoSpin RNA Clean-up (250) 250 preps for clean-up of total RNA from reaction mixtures - NucleoSpin RNA Columns, Collection Tubes, buffers
INDICAL INC INDISPIN PATHOGEN KIT 50
The IndiSpin Pathogen Kit is designed for high-quality and efficient extraction of pathogen nucleic acids, including viral RNA and DNA, and bacterial DNA. It is suitable for use with a wide variety of sample types such as whole blood, serum, plasma, other body fluids, swabs, washes, and tissue homogenates. Key features of the kit include:¿A unified protocol for both DNA and RNA extraction.¿Effective removal of inhibitors and contaminants for high-purity results.¿Compatibility with automation on the QIAcube for low to medium throughput workflows.¿A centrifugation-based protocol for straightforward implementation.The purified nucleic acids are free of proteins, nucleases, and other impurities, making them ideal for downstream applications, including real-time PCR-based pathogen detection. This versatile kit is particularly well-suited for applications in fields such as veterinary diagnostics, where sample types can vary significantly.
INNOVEX BIOSCIENCES BACKGROUND BUSTER READY-TO-USE 1 LITER
Number of slides: 11,200-16,000; Shelf life: 3 years; Peptide Blocker delivers complete eradication of background staining of animal, human tissues & cells stained by IHC, IF, in situ ( frozen, paraffin) & free floating sections; Short incubation step. A must for Mouse-on-Mouse staining.