There is no need for organic denaturants or chloroform. Instead, the kit features Zymo-Spin column technology and employs a single-buffer system that allows for efficient oligonucleotide adsorption to the matrix of Zymo-Spin™ IC Column. Oligonucleotide is washed and concentrated into a small volume of water ( 6 l). Purified oligonucleotide, available in just 2 minutes, is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.