DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - Bronchitis: Lung, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Control Genomic DNA - Bovine Male, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biotium Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) (CALD1/1424R), CF647 conjugate, 0.1mg/mL
Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low
Bioworld Blood Genomic DNA Isolation Mini, 50 Prep(s)
For the rapid preparation of high quality DNA from whole blood, plasma and serum Input volumes of 200 µL to 500 µL No phenol required for DNA isolation Extract DNA from whole blood, plasma and serum Fast and convenient spin column protocol FEATURES AND BENEFITSNo Phenol-Chloroform extraction or alcohol precipitation - Isolate genomic DNA without the use of harmful chemicals Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 30 minutes Isolate genomic DNA from various inputs - Isolate DNA from various blood inputs including plasma, whole blood and serum Recovered genomic DNA is suitable for downstream applications - Purified genomic DNA is fully compatible with restriction enzyme digestions, Southern Blot and PCR analysis. High quality DNA with no RNA contamination - No contamination or degradation of genomic DNA isolated with the Blood Genomic DNA Isolation Mini Kit is observed.
Biochain Institute Inc Genomic DNA - Plant Normal Tissue: Corn, 10 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Mouse Normal Tissue: Kidney, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Mouse Normal Tissue: Spleen, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Beckman Coulter DNAdvance Kit - Genomic DNA extraction from tissue, 9,600 preps (A48706)
Beckman Coulter DNAdvance Kit (A48706) is a high-throughput genomic DNA extraction reagent kit that uses SPRI paramagnetic bead chemistry to isolate high-quality DNA from mammalian tissue samples. It is designed for automation on liquid-handling workstations and produces DNA compatible with PCR, qPCR, SNP genotyping, and next-generation sequencing.
- High-throughput capacity: 9,600 preps per kit.
- Technology: SPRI paramagnetic bead-based DNA isolation.
- Sample types: mammalian tissue including brain, kidney, liver, lung, and tail.
- Throughput: processes three 96-well plates in ~1 hour using automation; 15 minutes hands-on time for 96 samples; 1 hour 46 minutes total run time for a 96-well plate.
- Typical yield: isolates up to 40 μg DNA per prep.
- Compatible with downstream analyses: PCR, qPCR, SNP genotyping, sequencing.
Alkali Scientific Plasmid DNA Spin Columns Polyp
Alkali ScientificPlasmid DNA Spin Columns provide a fast reliable and efficient solution for extracting high-quality plasmid DNA Supplied with column tubes and collection tubes these spin columns streamline the purification process ensuring high yields and exceptional purity They are suitable for a wide range of applications including cloning sequencing and transfection and are compatible with various spin-type column kits The columns are designed to extract nucleic acids from diverse sample types such as tissues cells plants and blood delivering large fragment sizes with reproducible results Key features include DNase- and RNase-free construction ensuring sample integrity and human DNA-free assurance making them ideal for sensitive downstream applications Large Fragment Size and High Purity Ensures optimal performance in molecular biology workflows High Quality and Reproducibility Provides consistent yields across different sample types Broad Sample Compatibility Suitable for t
Sigma Aldrich Fine Chemicals Biosciences Inactivated Escherichia coli Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml | 1ML
Inactivated Escherichia coli Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml | 1ML
NORLAND PRODUCTS INC HIPURE LIQUID GELATIN
HiPure Fish Gelatin pourable liquid 1 pintPrice for 12 or more.
Omega Biotek Inc Mag-Bind SeqDTR, Magnetic Bead-based purification system, 500 mL, Manual or automated, 96-well, 384-well, Over 800 bps
The Omega Bio-tek Mag-Bind SeqDTR is a magnetic bead-based purification system designed for the efficient and reliable removal of unincorporated dye terminators from sequencing reactions. This product employs proprietary chemistry and utilizes the unique properties of paramagnetic beads, enhancing sample purity and quality in sequencing applications. It is suitable for both manual and automated workflows, allowing users to achieve optimal performance with ease.
- Application: Removal of unincorporated dye terminators from sequencing reactions
- Technology: Magnetic bead based
- Kit volume: 500 mL
- Automation compatibility: Manual or automated
- Average read lengths: Over 800 bps (Min Phred 20)
- Reduce reagent usage: 1/16 or 1/32 compared to sephadex cleanups
- Contaminants removed: Nucleotides, primers, salts, dye terminators
- Storage conditions: 2-8 °C
- Processing time: Less than 25 minutes for 4 plates