DNA Extraction and Purification
Genelink Linear Acrylamide Solution (Linear polyacrylamide, LPA; 5mg/mL); 1 mL
Effective DNA and RNA precipitation with the use of monovalent salts and ethanol or isopropanol is dependent on its concentration. DNA and specifically RNA precipitation at a concentration of less than 50 ng/mL is usually not quantitative and the pellet is not clearly visible leading to unreliable recovery. Addition of linear acrylamide as a carrier/coprecipitant aids in quantitative recovery and specifically clear visibility pf the pellet. Linear acrylamide does not interfere with spectrophotometric readings, electrophoresis, and most molecular biology enzymatic applications including PCR. Linear acrylamide is prepared synthetically and is guaranteed free of all nucleic acids, DNase, RNase and proteases.
BIOSEARCH TECHNOLOGIES INC (Lucigen) MasterPure Complete DNA & RNA Purification Kit, 200 PurifQuickly purify high yields of high-molecular-weight genomic DNA, total cellular RNA or Total Nucleic Acid (TNA) with one kit.
The MasterPure™ Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types.
Macherey-Nagel MACHEREY-NAGEL NucleoBond HMW DNA Kit, High Integrity DNA Purification, Enzymatic Lysis Included, 20 Preps
MACHEREY-NAGEL NucleoBond HMW DNA kit is designed for the purification of high molecular weight DNA, ensuring high integrity suitable for long-read and Next Generation Sequencing applications. The kit utilizes anion-exchange chromatography and includes Proteinase K for gentle enzymatic lysis, minimizing DNA shearing. It is ideal for isolating DNA from cultured cells, tissue, and plant samples, making it a versatile choice for demanding research environments.
Sigma Aldrich Fine Chemicals Biosciences REDExtract-N-Amp(TM) Tissue PCR Kit | 100RXN
REDExtract-N-Amp(TM) Tissue PCR Kit | 100RXN
Zymo Research Corporation ZymoBIOMICS™ Microbial Community DNA Standard (2000 ng)
One of the major challenges in the emerging field of microbiomics is the bias and errors introduced in the complex workflows. Besides nucleic acid purification, bias also arises from sequencing library preparation and subsequent processes. The ZymoBIOMICS Microbial Community DNA Standard is designed to assess bias, errors and other artifacts after the step of nucleic acid purification. This DNA standard is created by pooling DNA extracted from pure cultures. It has an accurately defined composition, negligible impurities (0.01%), and contains genomes of a wide range of GC content (15% - 85%). This DNA standard is designed to have the same microbial composition with the cellular version, the ZymoBIOMICS Microbial Community Standard, so that they can be more powerful when working in tandem.
Zymo Research Corporation ZymoPURE™ Wash 2 (Concentrate) (23 ml)
ZymoPURE™ Wash 2 Concentrate (23 ml). Assists in washing ZymoPURE™ columns for subsequent purification of nucleic acid.
Zymo Research Corporation ZymoPURE™ Wash 1 (55 ml)
ZymoPURE™ Wash 1 (55 ml). Assists in washing ZymoPURE™ columns for subsequent purification of nucleic acid.
![Zymo Research Corporation ZymoBIOMICS DNA Microprep Kit (50 preps)Includes: [D4305] ZymoBIOMICS Microprep Kit x 1 [S6012-50] ZR BashingBead Lysis Tubes 50 pack x 1[D4300-1-40] ZymoBIOMICS Lysis Solution, 40 ml x 1](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-D4301ZYMOBIOMICSDNAMICROPREPKIT.JPG-250.jpg)
Zymo Research Corporation ZymoBIOMICS DNA Microprep Kit (50 preps)Includes: [D4305] ZymoBIOMICS Microprep Kit x 1 [S6012-50] ZR BashingBead Lysis Tubes 50 pack x 1[D4300-1-40] ZymoBIOMICS Lysis Solution, 40 ml x 1
The ZymoBIOMICS DNA Kits are designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Zymo Research Corporation ZymoPURE Wash 2 (Concentrate), 28 ml
ZymoPURE™ Wash 2 Concentrate (28 ml). Assists in washing ZymoPURE™ columns for subsequent purification of nucleic acid.
Zymo Research Corporation ZymoBIOMICS Lysis Solution, 40 ml
Used in combination with the ZR BashingBead Lysis Tubes, it assists with microbial lysis. It is certified to be low-bioburden to ensure minimal background contaminants for low abundance microbiome samples.
Invent Biotechnologies Inc Minute™ Detergent-Free Single Nuclei Isolation Kit, Spin-column based, 20 tests
Minute™ Detergent-Free Single Nuclei Isolation Kit, Spin-column based, 20 tests
Zymo Research Corporation Quick-DNA Fungal/Bacterial Microprep Kit, 50 Preps
The Quick-DNA Fungal/Bacterial Microprep Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.