DNA Extraction and Purification
New England Biolabs, Inc. NEBNext® Quick Ligation™ Module – 20 reactions
This module is part of the original standard library prep workflow, which is longer than the NEBNext Ultra II DNA Library Prep Kit workflow, has more components, and requires more clean-up steps. It also requires higher input amounts: 1 - 5 µg of input DNA vs. 500 pg - 1 µg required for the Ultra II DNA workflow. This module is also compatible with some Oxford Nanopore Technologies workflows.
- Compatible with some Oxford Nanopore Technologies workflows
New England Biolabs, Inc. NEBNext Library Quant Kit for Illumina – 500 reactions
The NEBNext Library Quant Kit components have been optimized to deliver significant improvements to qPCR-based library quantitation for Illumina sequencing. The kit contains primers which target the P5 and P7 Illumina adaptor sequences, and a set of four high-quality, pre-diluted DNA standards to enable reliable quantitation of diluted DNA libraries between 150–1000 bp.
STEMCELL Technologies Gel and PCR Clean-up Kit, Size: 250 Preparations
The Gel and PCR Clean-up Kit uses a membrane-based system to quickly purify DNA fragments of 100 bp to 10 kb in length from polymerase chain reaction (PCR), restriction enzyme digestion, or excised agarose gel fragments (up to 350 mg). The purified DNA is directly suitable for applications such as automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion, or in vitro transcription. Up to 40 µg of DNA can be processed per column in as little as 20 minutes and eluted in volumes as low as 15 µL, with up to 95% recovery depending on DNA fragment length. The Gel and PCR Clean-up Kit can be used to purify linear DNA fragments, supercoiled plasmid DNA, or single-stranded DNA (circular or linear).
Biochain Institute Inc PureSil-Silica Magnetic Beads, 10 mL/pk
PureSil-Silica Magnetic Beads are Fe3O4 magnetic beads coated with a silicon dioxide (SiO2) layer. Since silica is able to bind to the nucleic acids, PureSil-Silica Magnetic Beads serve as a simple and efficient tool for plasmid DNA purification for transfection or sequencing applications, genomic DNA purification for research or clinical applications, RNA purification for qPCR analysis, or PCR product clean-up for downstream analysis.Features- Capacity: 4mg DNA/ml beads- 1-2 um particle size- Bead concentration: 4mg/ml- Bead density: ~1.03g/cm3- Simple, short procedure- No costly columns required- No time consuming centrifugations requiredApplications- Plasmid DNA isolation- Genomic DNA isolation- RNA purification for qPCR- PCR product clean up
Biochain Institute Inc FFPE Tissue DNA Extraction Kit - Column, 1 kit/pk
BioChain’s FFPE Tissue DNA Extraction Kit - Column allows for facile and efficient deoxyribonucleic acid extraction from FFPE tissues, with potential high throughput capabilities and full compatibility for downstream applications such as qPCR. Utilizing heat and proteinase K treatment, BioChain’s FFPE Tissue DNA Extraction Kit- Column is optimized in the removal of paraffin, partial reversal of formalin cross linking, and release of DNA from fixed tissues. After clean up with DNA binding columns, the concentration of the high purity DNA can be determined by spectrophotometer or nanodrop equipment.
Biochain Institute Inc SeqPure PCR Purification Kit (500 ml), 500 ml/pk
SeqPure PCR reagent is a post PCR and Next Gen library prep clean up system based on paramagnetic beads technology, designed for an efficient purification of PCR amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic beads particles; and highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.
Zymo Research Corporation MagBead DNA/RNA Wash 1, 120 ml
MagBead DNA/RNA Wash 1 for use with several Zymo Research Magnetic bead based kits for high-throughput automation of DNA and RNA purification.
Zymo Research Corporation EZ-96 DNA Methylation™ Kit (Shallow-Well) (2 x 96 Rxns)
The EZ-96 DNA MethylationTM Kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. The products innovative desulphonation technology eliminates otherwise cumbersome precipitations.The kit is designed to reduce template degradation, minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
Macherey-Nagel NucleoBond PC 10000 EF (5)
NucleoBond PC 10000 EF (5) 5 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond AX 10000 Columns, NucleoBond Bottle Top Filters Type 2, buffers, RNase A
Zymo Research Corporation ZR-96 DNA Clean & Concentrator™-5 Kit (2 x 96 Preps)
It can also be used for post-RT cDNA clean-up and purification of sequencing-ready DNA from M13 phage. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the wells of the supplied Zymo-Spin™ I-96 Plate. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin technology to yield DNA that is free of salts andcontaminants in just minutes. DNA purified using the ZR-96 DCC-5 is suitable for nucleotide sequencing, microarray analysis, PCR, and restriction endonuclease digestion procedures.
Macherey-Nagel NucleoBond Xtra Midi, 50 preps
NucleoBond Xtra Midi (50) 50 preps for the isolation of plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A
Zymo Research Corporation MagBinding Beads, 8 ml
MagBinding Beads for use with Zymo Research magnetic bead-based purification kits. Sold separately or as part of a complete magnetic bead purification kit.
Cytek Biosciences Inc Second Step Reagents APC Streptavidin ..... Cat. No. 20-4317-U500 ..... Size 500 µg
Second Step Reagents APC Streptavidin ..... Cat. No. 20-4317-U500 ..... Size 500 µgAPC Streptavidin is a second-step reagent useful when detecting biotinylated antibodies in indirect staining protocols. Streptavidin is a nonglycosylated biotin-binding protein that is bacterially derived. Like avidin, it binds to biotin with high affinity but displays less nonspecific binding.