DNA Extraction and Purification
Biochain Institute Inc Express Cloning Checker Kit II: Green Solution, 1 ml/PK
BioChain's Express Cloning Checker Kits provide highly efficient methods of identifying recombinant colonies after transformation eliminating time-consuming plasmid preparation. In the large-scale screen method, the partial colonies of E. coli bacteria can be directly transfered from the transformation plates into the solutions of the kits and immediately run on an agarose gel electrophoresis. The results will be known within 1-2 hours instead of the 2 days required in a conventional method of labor-intensive plasmid DNA miniprep or colony hybridization.
Biochain Institute Inc Genomic DNA - Depression: Brain: Thalamus, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Zymo Research Corporation Human Methylated & Non-Methylated (WGA) DNA Set (DNA w/ primers)
The Human WGA Methylated & Non-methylated DNA Set isgenerated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CGdinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.
Zymo Research Corporation EZ-96 DNA Methylation MagPrep (4x96 rxns.)
The EZ-96 DNA Methylation MagPrep features a high-throughput (96-well), simplified procedure that streamlines bisulfite conversion of DNA. The kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Zymo Research Corporation Quick-DNA/RNA FFPE Kit (50 Preps)
The unique chemistries of the product have been optimized for maximum recovery of DNA and of both large and small RNA species. Simply deparaffinize tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin™ Column technology. The result is high-quality DNA & total RNA (including small RNAs) that is DNA-free and is ready for RT/qPCR, hybridization, sequencing, etc.
Research Products International Corp DNA Clean & Concentrator-5 w/Zymo-Spin I Columns, Uncapped, 200 preps
The DNA Clean & Concentrator-5 is a PCR purification kit that provides purification of up to 5 µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.
Clean and concentrate up to 5 µg DNA with ≥ 6 µl elution in as little as 2 minutes with 0 µl wash residue carryover.
Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
New England Biolabs, Inc. Monarch® DNA Capture Beads - 200 beads
These specialized glass beads are optimized for capturing HMW DNA in the Monarch HMW DNA workflows.
- A component of the Monarch HMW DNA Extraction Kits
- 4 mm borosilicate glass beads
- Optimized for extraction of HMW DNA
- Smooth surface to maximize elution efficiency
- Easy to handle when coupled with the Monarch Bead Retainers
New England Biolabs, Inc. Monarch® gDNA Nuclei Prep & Lysis Buffer Pack - 1 pack
The Monarch gDNA Nuclei Prep & Lysis Buffer Pack contains two lysis buffers used in the Monarch HMW DNA Extraction Kit for Cells & Blood. The Nuclei Prep Buffer is a mild lysis buffer used first to lyse the cell membrane and release the contents of the cytoplasm while the nuclei are kept intact. It is used pre-mixed with the RNase A enzyme included in the kit. After the RNA from the cytoplasm is digested by RNase A, the Nuclei Lysis Buffer, pre-mixed with the included Proteinase K, is used to lyse the nuclei and release the HMW genomic DNA. This 2-step lysis ensures that RNA in the cytoplasm is effectively degraded before the viscous genomic DNA from the nuclei is released. 20 ml of each buffer is included in this buffer pack. The Nuclei Prep Buffer is used chilled to 4°C, and it can be stored at 4°C for convenience if desired.
- Supplied as components of the Monarch HMW DNA Extraction Kit for Cells & Blood
Zymo Research Corporation Quick-DNA™ 96 Kit™ (4 x 96 Preps)
This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, plasma, buffy coat, buccal cells, cells from culture, and many biological liquid samples.For processing, simply add the specially formulated Genomic Lysis Buffer to the samples, vortex, and transfer the mixtures to the wells of the supplied SiliconA™ Plate. There is no need for organic denaturants or Proteinase K digestion because of the unique chemistries featured in the kit. Instead, the product yields high-quality, purified DNA in just minutes. PCR inhibitors are effectively removed during the purification process. DNA purified using the Quick-DNA™ 96 Kit is suitable for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
Norgen Biotek Corp Phage DNA Isolation Kit, 50 Prep
This kit provides a rapid spin column method for the purification of total DNA from a broad spectrum of bacteriophages propagated in bacteria grown in liquid cultures. The DNA is isolated without the use of phenol, chloroform or cesium chloride banding procedures. The spin-column based procedure is rapid and can be completed in less than 45 minutes. The kit is highly efficient for processing small volumes of phage supernatant (500 µL – 1 mL) and with the optional DNase and Proteinase K treatments phage DNA yields are maximized while host DNA contamination is minimized. Purified total phage DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), sequencing, cloning, Southern Blot and more.
Zymo Research Corporation ZymoBIOMICS 96 MagBead DNA Kit – Dx (No Lysis Matrix) (2 x 96 preps)
The ZymoBIOMICS DNA Kits are designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
LGC GENOMICS LLC (Lucigen) 1000mL; Simple, rapid extraction of PCR-ready DNA for screening and genotyping type applications from hair follicles, quill-end cells of feathers, tissue-culture cells, buccal cells and other samples
QuickExtract DNA Extraction Solution - 1000mLSimple, rapid extraction of PCR-ready DNA for screening and genotyping type applicationsFast: 8 minute extraction protocol for most sample typesSimple: No centrifugation steps of spin columns used to help increase yieldsAutomation-friendly: Simple protocol integrated easily into automated workflowsSafe: Uses only non-toxic reagentsRecommended for rapid, easy sample prep for T7E1 CRISPR mutation detection assays
Biochain Institute Inc Genomic DNA - Progressive Supranuclear Palsy: Brain: Cerebellum, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.