DNA Extraction and Purification
STEMCELL Technologies Gel and PCR Clean-up Kit, Size: 250 Preparations
The Gel and PCR Clean-up Kit uses a membrane-based system to quickly purify DNA fragments of 100 bp to 10 kb in length from polymerase chain reaction (PCR), restriction enzyme digestion, or excised agarose gel fragments (up to 350 mg). The purified DNA is directly suitable for applications such as automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion, or in vitro transcription. Up to 40 µg of DNA can be processed per column in as little as 20 minutes and eluted in volumes as low as 15 µL, with up to 95% recovery depending on DNA fragment length. The Gel and PCR Clean-up Kit can be used to purify linear DNA fragments, supercoiled plasmid DNA, or single-stranded DNA (circular or linear).
Takara Bio NucleoSpin® Gel and PCR Clean-Up, 50 preps
NucleoSpin Gel and PCR Clean-up (50) 50 preps for PCR clean-up and gel extraction - NucleoSpin Gel and PCR Clean-up Columns, Collection Tubes, buffers.
Macherey-Nagel Buffer A1 (1000 mL)
Buffer A1 (1000 mL) Resuspension Buffer A1, without RNase A bottle of 1000 mL
New England Biolabs, Inc. RNase H – 1250 units
RNase H (Ribonuclease H ) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.
Macherey-Nagel NucleoSpin Plant II, 250 preps
NucleoSpin Plant II (250) 250 preps for the isolation of genomic DNA from plant - NucleoSpin Plant II Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase A
Macherey-Nagel NucleoBond Xtra Maxi EF, 10 preps
NucleoBond Xtra Maxi EF (10) 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A
Omega Biotek Inc Plasmid DNA Mini Kit I, (V-spin), 50 preps
Isolate up to 25 ug plasmid DNA from 1-5 mL bacterial cultures using mini spin columns.
Sigma Aldrich Fine Chemicals Biosciences GenElute HP Plasmid Maxiprep Kit sufficient for 25purifications
GenElute HP Plasmid Maxiprep Kit sufficient for 25purifications
Apexbio Technology LLC ApexPrep DNA Plasmid Miniprep Kit 300 tests
The ApexPrep DNA Plasmid Miniprep Kit is designed for isolation of plasmid DNA through alkaline-mediated bacterial cell lysis Upon lysis plasmid DNA selectively binds to a silica-based spin column under high-salt conditions separating it from cellular debris and contaminants Subsequent washing removes proteins and organic impurities yielding purified plasmid DNA (up to 30 g) from bacterial culture volumes of 1 5 mL Extracted plasmid DNA is suitable for downstream molecular biology applications including restriction digestion PCR amplification sequencing cloning transfection and in vitro transcription or translation assays
New England Biolabs, Inc. NEBNext Library Quant Kit for Illumina – 500 reactions
The NEBNext Library Quant Kit components have been optimized to deliver significant improvements to qPCR-based library quantitation for Illumina sequencing. The kit contains primers which target the P5 and P7 Illumina adaptor sequences, and a set of four high-quality, pre-diluted DNA standards to enable reliable quantitation of diluted DNA libraries between 150–1000 bp.
Macherey-Nagel NucleoSpin DNA RapidLyse, 50 preps
NucleoSpin DNA RapidLyse (50) 50 preps for the isolation of genomic DNA - NucleoSpin DNA RapidLyse Columns, Collection Tubes, buffers, Proteinase K
Boca Scientific Inc Buccal-Prep Plus DNA Isolation Kit, 50 Extractions
The Buccal-Prep Plus DNA Isolation Kit is a precipitation-based kit combining all the necessary optimized buffers to fully lyse and simply purify DNA from buccal swabs. The kit has a fast protocol that produces high yields and purity for all downstream processing applications. As with our other precipitation-based chemistries, no solvents, alcohol, or other external buffers are required, giving fewer processing steps and creating a faster process time.