RNA Preparation and Purification
New England Biolabs, Inc. G(5')ppp(5')A RNA Cap Structure Analog – 1 umol
The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level. For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation, and a decrease in the formation of the initiation complex of mRNAs for protein synthesis. Certain prokaryotic mRNAs containing a 5' terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system. Also a cap requirement has been observed for splicing eukaryotic substrate RNAs. A method using E. coli RNA Polymerase primed with m7G(5' )ppp(5' )G or m7G(5' )ppp(5' )A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas. Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA polymerase primed with m7G(5' )ppp(5' )G.
Zymo Research Corporation Direct-zol™ RNA PreWash (Concentrate) (160 ml)
Direct-zol™ RNA Pre-wash (160 ml) for use with Direct-zol™ RNA Purification Kits for rapid isolation of high-quality total RNA including small RNAs (>17 nt) from samples in TRI-Reagent, TRIzol or similar.
Zymo Research Corporation Quick-RNA Plant MiniPrep™ Kit (50 Preps)
The kit includes unique technology such as the ZR BashingBead™ Lysis Tubes and features a specially formulated RNA Lysis Buffer. The Zymo-Spin™ IIICG Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin™ IICR Column efficiently adsorbs total RNA.The RNA is washed and then eluted with DNase/RNase-Free Water. For inhibitor removal, the eluted RNA can be treated by running the sample through the Zymo-Spin™ III-HRC Filters. RNA is eluted in as little as 6 l and is suitable for subsequent procedures including RT-qPCR.
Zymo Research Corporation ZR small-RNA™ PAGE Recovery Kit (20 Preps)
The ZR small-RNA™ PAGE Recovery Kit is a refinement of the crush & soak method that incorporates a unique buffer system together with Zymo-Spin™ Column technology for improved recovery and added convenience. The recovered RNA can be concentrated at elution step in volumes as small as 6 l and is ideal for any downstream enzymatic reaction or manipulation.
Zymo Research Corporation Direct-zol-96 RNA (4x96 Preps)
by using this product, total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety ofsample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.)The extraction method inactivates viruses and other infectious agents.The procedure is easy: simply apply a sample in TRI Reagent to the Zymo-Spin™ I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is high-quality RNA suitable for subsequent RNA-based methods including Next-Gen sequencing, RT-qPCR, hybridization, etc.
Roche Diagnostics LIGHTCYCLER RNA MASTER SYBR GR
LightCycler RNA Master SYBR Green I - 1 kit (for 96 reactions of 20 uL final reaction volume)
Macherey-Nagel NucleoSpin RNA Plant, 250 preps
NucleoSpin RNA Plant (250) 250 preps for the isolation of RNA from plant - NucleoSpin RNA Plant Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase
Macherey-Nagel NucleoSpin RNA Clean-up XS, 50 preps
NucleoSpin RNA Clean-up XS (50) 50 preps for the clean-up of total RNA - NucleoSpin RNA XS Columns, Collection Tubes, buffers
Norgen Biotek Corp RNA Clean-Up and Concentration Kit, 50 Prep
This kit is designed to clean-up and concentrate RNA (including miRNA) from TRIzol and TRI Reagent, enzymatic reactions, in vitro transcription, labelling reactions, etc. This is a robust kit for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications.The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.The kit elutes RNA in 20 µL for all types of downstream applications. For smaller elution volumes of 8 µL, please refer to the microspin column kits such as the RNA Clean-Up and Concentration Micro-Elute Kit for the highest concentration of RNA sample.
New England Biolabs, Inc. NEBNext Magnesium RNA Fragmentation Module – 200 reactions
Fragmentation of RNA can be performed by enzymes, heat alone, or exposure to divalent cations at elevated temperature. This kit provides highly efficient and tunable RNA fragmentation by incubation with Magnesium ions at 94°C.
