RNA Preparation and Purification
Research Products International Corp ZR-96 Quick-RNA Kit, 4 x 96 Preps
The Quick-RNA 96 Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 106) and tissue samples (up to 5 mg). The procedure combines a unique buffer system with Zymo-Spin plate technology to yield high quality total RNA (including small RNAs ~17-200 nt) in about 30 minutes. The procedure is simple: Add the provided RNA Lysis Buffer to a sample, then purify the RNA using the provided Silicon-A Plate. The result is highly-concentrated, DNA-free RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, sequencing etc.
Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
DNA-Free: Genomic DNA removal column and DNase I included.
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Biochain Institute Inc Total RNA - Rat Normal Tissue: Spleen, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Sigma Aldrich Fine Chemicals Biosciences RNA from yeast
RNA from yeast is the total RNA obtained from Saccharomyces cerevisiae.
Zyagen Labs Mouse C57 thyroid total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy C57BL/6 Mouse THYROID tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Zyagen Labs Pig Universal Reference Total RNA extracted from wide variety of male and female PIG tissues. Made at industrial scales to minimize lot-to-lot variation. Ideal for microarray, NB, PCR and many other applications. 4X0.1mg/pK at 1mg/ml
Zyagen Universal Reference Total RNA is composed of highly pure intact total RNA isolated from wide variety of male & female tissues. Total RNA is extracted using modified guanidine thiocyanate method, treated with DNase & packed at a concentration of 1mg/ml. Features: High quality intact RNA without genomic DNA contamination. Consistent control for standard data set comparisons. Extracted from large variety of male & female tissues for broad gene coverage. Made at industrial scales to minimize lot-to-lot variation Quality Control: The integrity of RNA sample as indicated by intact ribosomal RNA is verified by agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. Applications: Reference RNA is ideal for microarray analysis, Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, microRNA studies, & purification of mRNA for library construction.
![Zymo Research Corporation Direct-zol™ RNA MiniPrep (50 Preps) w/ Zymo-Spin™ IIC Columns (Capped) (Product Supplied w/ 50 ml TRI Reagent™) [Includes R2050 x 1, R2050-1-50 - packaged separately]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-R2051DIRECTZOLRNAMINIPREPYES.JPG-250.jpg)
Zymo Research Corporation Direct-zol™ RNA MiniPrep (50 Preps) w/ Zymo-Spin™ IIC Columns (Capped) (Product Supplied w/ 50 ml TRI Reagent™) [Includes R2050 x 1, R2050-1-50 - packaged separately]
Total RNA including small RNAs (17-200 nt)2, is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, samples stored in DNA/RNA Shield™, etc.).Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary. RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.
Zymo Research Corporation Quick-RNA™ MiniPrep Kit (50 Preps) w/Zymo-Spin™ IIICG Columns (Capped) & Spin-Away™ Filters
The procedure of Quick-RNA™ Miniprep Kit uses unique spin-column technology that results in highquality total RNA (including small/microRNAs) and is ready for Next-Gen Sequencing, RT/qPCR, hybridization, etcThe Quick-RNA™ Kits yields high quality total RNA.High levels of genomic DNA contamination arepresent in the preps from Suppliers Q & P but not withthe Quick-RNA™ Kits. Total RNA was isolated fromhuman epithelial cells.RNA isolated with the Quick-RNA™ Kits is DNA-free.Samples isolated with Supplier Q's kit are provided forcomparison. Total RNA was isolated from 106 humanepithelial cells (with in-column DNase treatments forboth kits). Each amplification curve represents anaverage of three independent isolation experiments.
Biochain Institute Inc Total RNA - Monkey (Rhesus) Normal Tissue: Liver, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Zyagen Labs Mouse C57 testis total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy C57BL/6 Mouse TESTIS tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Brain: Olfactory (region), 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Brain: Postcentral Gyrus, 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Rat Normal Tissue: Bladder, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Beckman Coulter Wash WBD reagent (C39489)
Beckman Coulter Wash WBD (C39489) is a liquid wash reagent used with Beckman Coulter diagnostic analyzers in automated wash cycles to remove residual reagents and help maintain assay performance.
- Provides instrument wash for automated analyzers.
- Helps remove residual reagent and reduce carryover.
- Intended for laboratory diagnostic use.
- Supplied as a liquid reagent for use in instrument wash systems.
New England Biolabs, Inc. 3'-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog – 1 umol
Blocking of the 3' -hydroxyl of m7G with 3' -0-Me assures that the capped transcripts are homogeneous. The 3' - hydroxyl of the non-methylated G is the only 3' - hydroxyl available for initiation. The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level. For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation, and a decrease in the formation of the initiation complex of mRNAs for protein synthesis. Certain prokaryotic mRNAs containing a 5 terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system. Also a cap requirement has been observed for splicing eukaryotic substrate RNAs. A method using E. coli RNA Polymerase primed with m7G(5' )ppp(5' )G or m7G(5' )ppp(5' )A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Stomach, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.