RNA Preparation and Purification
Molecular Research Center RNAZOL RT REAGENT 50ML
A single-step reagent isolating mRNA and small RNA in separate fractions. No chloroform-induced phase separation or refrigerated centrifuge required. No DNase treatment. Higher yields and quality than previous single-step methods.
Macherey-Nagel NucleoSpin RNA Plus XS, 50 preps
NucleoSpin RNA Plus XS (50) 50 preps for the isolation of total RNA - NucleoSpin RNA Plus XS Columns, NucleoSpin gDNA Removal XS Columns, Collection Tubes, buffers
New England Biolabs, Inc. 3'-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog – 1 umol
Blocking of the 3' -hydroxyl of m7G with 3' -0-Me assures that the capped transcripts are homogeneous. The 3' - hydroxyl of the non-methylated G is the only 3' - hydroxyl available for initiation. The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level. For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation, and a decrease in the formation of the initiation complex of mRNAs for protein synthesis. Certain prokaryotic mRNAs containing a 5 terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system. Also a cap requirement has been observed for splicing eukaryotic substrate RNAs. A method using E. coli RNA Polymerase primed with m7G(5' )ppp(5' )G or m7G(5' )ppp(5' )A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas.
Molecular Research Center BAN 100 ML
BAN is molecular biology grade 4-bromoanisole for phase separation in the TRI Reagent RT and RNAzol RT procedures.
Macherey-Nagel NucleoSpin RNA Clean-up XS, 50 preps
NucleoSpin RNA Clean-up XS (50) 50 preps for the clean-up of total RNA - NucleoSpin RNA XS Columns, Collection Tubes, buffers
Epoch Life Science Inc GenCatch™ Viral RNA Miniprep Kit (250 preps) Uniquely formulated buffers, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to purify viral nucleic acids from cell-free samples such as plasma, serum, body fluid, urine, and cell culture supernatant. This system utilizes guanidine thiocyanate and 2-mercaptoethanol for their capabilities to inactivate RNase and denature proteins. Purified viral RNA is suitable for applications such as RT-PCR, Northern and RNase protection assays. This system is ideal for isolation of RNA from pathogenic viruses such as hepatitis C virus (HCV) for RT-PCR screening of clinical samples.
Norgen Biotek Corp Plasma/Serum RNA Purification Midi Kit
This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a two column method. This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 250 µL to 1.5 mL. Plasma samples prepared from blood collected on heparin should not be used.The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL. All components for the purification and concentration are provided in one convenient and fast kit for the easy processing of large input volumes of bodily fluids.The purified plasma/serum RNA is fully compatible with all downstream applications including NGS and more
Roche Diagnostics DISCOVERY Wash (10X), 2 L
The ROCHE DIAGNOSTIC DISCOVERY Wash (10X) (RUO) is a concentrated wash solution tailored for research applications, featuring a 10X concentration that makes it essential for various experimental designs. This buffer is specifically crafted to enhance the performance of automated immunohistochemistry (IHC), in situ hybridization (ISH), and multiplexing procedures, thereby ensuring more reliable washing steps. This product is exclusively for research use and is not intended for diagnostic purposes, catering to the needs of scientists and researchers seeking dependable solutions for their workflows.
- 10X concentrated wash solution.
- Designed for research applications only.
- Compatible with automated IHC, ISH, and multiplexing.
- Ensures reliable washing performance.
- Suitable for use with DISCOVERY ULTRA and XT systems.
- Volume: 2 L.
System Biosciences LLC SeraMir Exosome RNA Purification Column Kit (20 exoRNA columns), 20 Preps
Efficiently purify RNA from already isolated exosomes with SBI’s most flexible SeraMir Kit.
Apexbio Technology LLC HyperScribe™ All in One mRNA Synthesis Kit (ARCA, T7, poly(A))(Synonyms: HyperScribe™ mRNA Kit, HyperScribe™ ARCA Kit, T7 ARCA-poly(A) mRNA Kit, All-in-One mRNA Synthesis Kit), 25 rxns.
HyperScribe All in One mRNA Synthesis Kit (ARCA T7 poly(A)) is designed for rapid in vitro generation of capped and polyadenylated mRNA transcripts Utilizing T7 RNA polymerase this system incorporates Anti-Reverse Cap Analog (ARCA) a Cap0 analog co-transcriptionally at the 5 terminus to confer mRNA stability and enhance translational efficiency After template DNA removal via DNase I treatment Poly(A) polymerase adds a poly(A) tail at the 3 -end further stabilizing the transcript and increasing translation initiation efficiency This kit is employed in research involving translation systems antisense RNA RNA interference RNA vaccines structural RNA studies and ribonuclease assays
