RNA Preparation and Purification
Biochain Institute Inc Total RNA - Human Adult Normal Tissue 5 Donor Pool: Kidney, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
New England Biolabs, Inc. NEBNext Ultra™ II Directional RNA Library Prep with Sample Purification Beads – 96 reactions
The Ultra II Directional RNA Library Prep Kit for Illumina delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1 µg. The NEBNext Ultra II Directional RNA Library Prep Kit derives its directionality from the “dUTP” method for strand-specificity, with proven superiority for this application. For convenience, this kit includes SPRISelect beads.
New England Biolabs, Inc. NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® – 96 reactions
The Ultra II Directional RNA Library Prep Kit for Illumina delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1 µg. The NEBNext Ultra II Directional RNA Library Prep Kit derives its directionality from the “dUTP” method for strand-specificity, with proven superiority for this application.
- Generate the highest yields of high quality libraries, with a broad range of input amounts
- Increase the complexity and transcript coverage of your libraries
- Optimize your time with streamlined workflows, reduced hands-on time, and automation compatibility
- Rely on robust performance, even with low quality RNA, including FFPE
New England Biolabs, Inc. Monarch® Spin RNA Cleanup Kit (50 ug) - 10 preps
The Monarch Spin RNA Cleanup Kit (50 ug) rapidly and reliably purifies up to 50 ug of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design helps ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 20 ul. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in downstream applications including RT-PCR, RNA library prep for NGS and RNA labeling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nts). Monarch Spin RNA Cleanup kits are also available for 10 ug and 500 ug binding capacities. Columns and buffers are also available separately for convenience
Molecular Research Center TRI REAGENT BD 100ML
For single-step isolation of total RNA or simultaneous isolation of RNA, DNA and proteins from whole blood, plasma, or serum. 0.75 ml of TRI Reagent BD processes 0.25 ml of blood derivatives.
New England Biolabs, Inc. Ribonucleotide Solution Mix – 50 µmol of each
Ribonucleotide Solution Mix is a buffered equimolar solution of ribonucleotide triphosphates: rATP, rCTP, rGTP and rUTP, pH 7.5 as sodium salts. The NTPs are also available as Ribonucleotide Solution Set . Supplied as: Sodium salts, pH 7.5 Reagents Supplied (small): NTP Mix (25 mM of each) 0.4 ml Reagents Supplied (large): NTP Mix (25 mM of each) 5 x 0.4 ml Diluent Compatibility: Can be diluted using nuclease-free water
Zymo Research Corporation Quick-RNA™ MiniPrep Plus Kit (200 Preps) w/Zymo-Spin™ IIICG Columns (Capped) & Spin-Away™ Filters
The Quick-RNA Miniprep Plus Kit is designed for the easy, reliable, and rapid isolation of DNA-free RNA from cells, all tissue types, whole blood, and biological fluids. The provided DNA/RNA Shield stabilizes samples, allowing them to be stored without the need for immediate freezing or processing for up to one month. Furthermore, DNA/RNA Shield™ inactivates RNases as well as microbial pathogens (viruses, bacteria, etc.). The procedure combines a unique buffer system with Zymo-Spin Column technology to yield high quality total RNA (including small RNAs 17-200 nt). Simply add DNA/RNA Shield and Proteinase K to extract total RNA from any tissue, then purify the RNA using the Zymo-Spin Column. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing, etc. In addition, the kit can be used for the enrichment of small and large RNAs in two separate fractions.
Zymo Research Corporation ZymoBIOMICS™ RNA Mini Kit (50 preps)
The ZymoBIOMICS RNA Miniprep Kit is designed for purifying RNA from a wide array of sample inputs that is ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The procedure uses Zymo-Spin Column technology that results in high-quality total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors and is ready for RT-PCR, hybridization, sequencing, etc. DNase I included.
Omega Biotek Inc 2 mL Collection Tubes, 5000/cs
2.0 mL Tube, Capless, graduated. 100/pack, 50 pack/case.
Roche Diagnostics KAPA mRNA HyperPrep (96rxn) Kit
KAPA mRNA HyperPrep Kits include all reagents required for mRNA Capture, RNA fragmentation, cDNA synthesis, library preparation and amplification. KAPA Pure Beads are supplied in each kit for reaction cleanups while KAPA Adapters are available separately. KAPA mRNA HyperPrep (96rxn) Kit includes three component kits: mRNA Capture 96 rxn Kit, RNA Hyper 96 rxn Kit, and 3 x 5mL Pure Beads. *This item is non returnable.
Research Products International Corp RNA Clean & Concentrator-5 w/Zymo-Spin IC Columns, Capped, 200 Preps
The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
![Zymo Research Corporation Direct-zol™ RNA MiniPrep (50 Preps) w/ Zymo-Spin™ IIC Columns (Capped) (Product Supplied w/ 50 ml TRI Reagent™) [Includes R2050 x 1, R2050-1-50 - packaged separately]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-R2051DIRECTZOLRNAMINIPREPYES.JPG-250.jpg)
Zymo Research Corporation Direct-zol™ RNA MiniPrep (50 Preps) w/ Zymo-Spin™ IIC Columns (Capped) (Product Supplied w/ 50 ml TRI Reagent™) [Includes R2050 x 1, R2050-1-50 - packaged separately]
Total RNA including small RNAs (17-200 nt)2, is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, samples stored in DNA/RNA Shield™, etc.).Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary. RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.
Zymo Research Corporation Direct-zol-96 RNA (4x96 Preps)
by using this product, total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety ofsample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.)The extraction method inactivates viruses and other infectious agents.The procedure is easy: simply apply a sample in TRI Reagent to the Zymo-Spin™ I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is high-quality RNA suitable for subsequent RNA-based methods including Next-Gen sequencing, RT-qPCR, hybridization, etc.