RNA Preparation and Purification
New England Biolabs, Inc. NEBNext RNA Depletion Core Reagent Set - 6 rxns
In RNA-seq, highly expressed transcripts with minimal biological interest, such as ribosomal RNA (rRNA) can dominate readouts and mask detection of more informative low-abundance transcripts. This challenge is amplified when working with sample types for which pre-designed RNA depletion kits are not available. The NEBNext RNA Depletion Core Reagent set provides a customized approach to deplete unwanted RNA from any organism, using probe sequences designed with the user-friendly NEBNext Custom RNA Depletion Design Tool. The efficient RNase-H-based workflow, and close tiling of probes designed using the online tool, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts. The kit is also available with RNAClean beads.
New England Biolabs, Inc. NEBNext Ultra™ II Directional RNA Library Prep with Sample Purification Beads – 96 reactions
The Ultra II Directional RNA Library Prep Kit for Illumina delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1 µg. The NEBNext Ultra II Directional RNA Library Prep Kit derives its directionality from the “dUTP” method for strand-specificity, with proven superiority for this application. For convenience, this kit includes SPRISelect beads.
New England Biolabs, Inc. Monarch® Spin RNA Cleanup Kit (10 ug) - 100 preps
The Monarch Spin RNA Cleanup Kit (10 ug) rapidly and reliably purifies up to 10 ug of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. The kit can also be applied for total RNA extraction from some sample types. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design helps ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 ul. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nts). Monarch Spin RNA Cleanup kits are also available for 50 ug and 500 ug binding capacities. Columns and buffers are also available separately for convenience
![Zymo Research Corporation Direct-zol-96 MagBead RNA (4x96 preps) w/ TRI Reagent [Includes R2050-1-200 x 2 – packaged separately]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502037264.JPG-250.jpg)
Zymo Research Corporation Direct-zol-96 MagBead RNA (4x96 preps) w/ TRI Reagent [Includes R2050-1-200 x 2 – packaged separately]
The extraction method inactivates viruses and other infectious agents. Total RNA (including small/microRNAs) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, DNA/RNA Shield™ samples, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRIzol/TRI Reagent, wash and elute the RNA. No chloroform, phase separation or precipitation steps are necessary. High-quality total RNA is ready for Next-Gen sequencing, RT-qPCR, transcription profiling, hybridization, etc
Roche Diagnostics DISCOVERY Wash (10X), 2 L
The ROCHE DIAGNOSTIC DISCOVERY Wash (10X) (RUO) is a concentrated wash solution tailored for research applications, featuring a 10X concentration that makes it essential for various experimental designs. This buffer is specifically crafted to enhance the performance of automated immunohistochemistry (IHC), in situ hybridization (ISH), and multiplexing procedures, thereby ensuring more reliable washing steps. This product is exclusively for research use and is not intended for diagnostic purposes, catering to the needs of scientists and researchers seeking dependable solutions for their workflows.
- 10X concentrated wash solution.
- Designed for research applications only.
- Compatible with automated IHC, ISH, and multiplexing.
- Ensures reliable washing performance.
- Suitable for use with DISCOVERY ULTRA and XT systems.
- Volume: 2 L.
New England Biolabs, Inc. Monarch® Spin RNA Cleanup Kit (500 ug) - 100 preps
The Monarch Spin RNA Cleanup Kit (500 ug) reliably purifies up to 500 ug of concentrated, high-quality RNA (>25 nt) from enzymatic reactions and in vitro transcription (IVT) reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our columns help ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 50 ul. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nt). Monarch Spin RNA Cleanup Kits are also available for 10 ug and 50 ug binding capacities. Columns and buffers are also available separately for convenience
Zymo Research Corporation Load N Go Quick-DNA/RNA HT Kit
The Load N' Go Quick-DNA/RNA HT kit offers a streamlined solution for high-throughput nucleic acid extraction from a variety of biological and clinical samples, such as swabs, biological liquids, and tissues. Compatible with robotic sample processors, this kit ensures the efficient isolation of high-quality DNA and RNA, making it suitable for sensitive downstream applications, including Next-Gen sequencing and RT/qPCR, ultimately enhancing the precision and reliability of scientific research.
Research Products International Corp Quick-RNA MiniPrep Kit w/Zymo-Spin IIICG Columns, Capped, & Spin-Away Filters, 50 Preps
The Quick-RNA Miniprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 107) and tissue samples (up to 50 mg). The procedure combines a unique buffer system with Zymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.
Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
DNA-Free: Genomic DNA removal column and DNase I included.