Miscellaneous Molecular Biology Reagents
Invitrogen™ Yeast tRNA (10 mg/mL)
Invitrogen Yeast tRNA purified from brewer's yeast. When used at a final concentration of 10 to 20 μg/mL, yeast tRNA is an effective coprecipitant to aid in recovery of small amounts of nucleic acids.
Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)
Sheared Salmon Sperm DNA is a very effective blocking agent when used in Northern prehybridization and hybridization buffers at a concentration of 100 to 200 μg/mL.
Thermo Scientific™ IPTG Solution, ready-to-use
Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.
Invitrogen™ SDS, 20% Solution, RNase-free
Invitrogen molecular biology grade, SDS, 20% Solution is supplied in one bottle containing 250 mL.
Invitrogen™ Yeast RNA (10 mg/mL)
Purified Torulla Invitrogen yeast RNA is suitable as a coprecipitant in nucleic acid precipitations. It cannot be used in reactions inhibited by exogenous RNA, and it is the most inexpensive source of a high-quality coprecipitant.
Thermo Scientific™ MgCl2 (magnesium chloride) (25 mM)
Thermo Scientific 25 mM MgCl2 is an aqueous solution of 0.22 μm membrane-filtered magnesium chloride, used for optimization of magnesium ion concentration in PCR.
Invitrogen™ DNase I Buffer (10X)
An Invitrogen component of the MAXIscript™ Kits, the buffer is supplied in one tube containing 1 mL.
Thermo Scientific™ X-Gal Solution, ready-to-use
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Thermo Scientific™ IPTG, dioxane-free
Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.
Thermo Scientific™ EDTA (0.5 M), pH 8.0
Optimize biochemistry or molecular biology applications that require a chelator for divalent cations.
Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)
In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing.
Invitrogen™ IPTG (Dioxane-Free)
Invitrogen IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant plasmids or phage.
Invitrogen™ Potassium Acetate (3 M), pH 5.5, RNase-free
Invitrogen Molecular biology grade, 3M Potassium Acetate, pH 5.5 solution is supplied in one bottle containing 100 mL.
Invitrogen™ SSC (20X), RNase-free
Molecular biology grade, Invitrogen 20X SSC solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use.
Thermo Scientific™ Formamide
Prepare hybridization cocktails and various denaturing solutions used with nucleic acids using this highly pure formulation of formamide denaturant.