Miscellaneous Molecular Biology Reagents
Thermo Scientific™ Agarose I (Molecular Biology Grade)
Ensure uniform lot-to-lot consistency in preparing electrophoresis gels using highly purified and blended regular- or low-melting agarose formulations.
MilliporeSigma™ Calbiochem™ Polyvinylpyrrolidone
Polymer used as a pharmaceutical aid, complexing agent and solubilizer
Thermo Scientific™ Phenol:Chloroform + Tris Buffer
Perform DNA extractions with this phenol:chloroform:isoamyl alcohol mixture (25:24:1), accompanied by a buffer to equilibrate the solution at either pH 6.7 or pH 8.0.
Thermo Scientific™ DTT (dithiothreitol)
Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.
Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)
Sheared Salmon Sperm DNA is a very effective blocking agent when used in Northern prehybridization and hybridization buffers at a concentration of 100 to 200 μg/mL.
Invitrogen™ Yeast tRNA (10 mg/mL)
Invitrogen Yeast tRNA purified from brewer's yeast. When used at a final concentration of 10 to 20 μg/mL, yeast tRNA is an effective coprecipitant to aid in recovery of small amounts of nucleic acids.
Thermo Scientific™ IPTG Solution, ready-to-use
Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.
Invitrogen™ SDS, 20% Solution, RNase-free
Invitrogen molecular biology grade, SDS, 20% Solution is supplied in one bottle containing 250 mL.
Invitrogen™ Ammonium Acetate (5 M), RNase-free
Invitrogen Molecular biology grade, 5 M Ammonium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.
Thermo Scientific™ IPTG, dioxane-free
Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.
Thermo Scientific™ X-Gal Solution, ready-to-use
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Invitrogen™ Formamide (Deionized)
Ambion formamide has been deionized, is nuclease-free, and comes packaged as a liquid in an amber glass bottle under argon.
Thermo Scientific™ EDTA (0.5 M), pH 8.0
Optimize biochemistry or molecular biology applications that require a chelator for divalent cations.
Thermo Scientific™ MgCl2 (magnesium chloride) (25 mM)
Thermo Scientific 25 mM MgCl2 is an aqueous solution of 0.22 μm membrane-filtered magnesium chloride, used for optimization of magnesium ion concentration in PCR.
Invitrogen™ SSC (20X), RNase-free
Molecular biology grade, Invitrogen 20X SSC solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use.