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Nucleotides are composed of a nitrogenous base covalently bound to a 5-carbon sugar molecule and one or more phosphate functional groups. They are the molecules that make up the structural units of DNA and RNA.
Tribioscience’s ADP/ATP Ratio Assay kit utilizes bioluminescent detection of the ADP and ATP levels for rapid screening of apoptosis, necrosis, growth arrest, and cell proliferation simultaneously in mammalian cells. Applications - Cell Proliferation. Cytotoxicity and Apoptosis. HTS. Key Features - Sensitive and Accurate - As low as 100 cells can be accurately quantified. Simple and High-Throughput - Simple procedure; Can be readily automated on HTS liquid handling systems.
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A metabolite of nicotinamide; formed via oxidation of nicotinamide by CYP2E1; inhibits proliferation and induces reduction of NBT in HL-60 promyelocytic leukemia cells at 30 mM; levels in urine are elevated in a mouse model of high-fat diet-induced obesity
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A metabolite of nicotinamide; formed via oxidation of nicotinamide by CYP2E1; inhibits proliferation and induces reduction of NBT in HL-60 promyelocytic leukemia cells at 30 mM; levels in urine are elevated in a mouse model of high-fat diet-induced obesity
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A metabolite of nicotinamide; formed via oxidation of nicotinamide by CYP2E1; inhibits proliferation and induces reduction of NBT in HL-60 promyelocytic leukemia cells at 30 mM; levels in urine are elevated in a mouse model of high-fat diet-induced obesity
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5-Br-UTP is a chemically modified ribonucleoside triphosphate that incorporates a bromine substituent at the 5 position of uracil Upon enzymatic incorporation into RNA sequences this compound confers enhanced structural stability and tolerance to enzymatic degradation by nucleases Such modified ribonucleotides are frequently employed in SELEX (Systematic Evolution of Ligands by EXponential enrichment) methodologies to generate aptamers exhibiting improved resistance to nucleolytic cleavage and greater thermal robustness Consequently 5-Br-UTP holds relevance for RNA-based therapeutic research and aptamer engineering applications
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Aminoallyl-dUTP (CAS 116840-18-7) is a modified deoxynucleotide designed for indirect covalent labeling of DNA or cDNA in molecular biology research Structurally analogous to natural dTTP it incorporates into DNA via enzymatic reactions including PCR (Taq polymerase) nick translation primer extension (Klenow exo-) terminal labeling (TdT) and reverse transcription (MMLV RT) The introduced primary amine moiety enables subsequent conjugation to NHS-activated labels such as fluorophores for fluorescence microscopy or biotin for affinity-based purification and detection strategies including in situ hybridization
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Deoxynucleotide (dNTP) Solution Mix is an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP. A small pack contains 4 vials of 0.2 mls and a large pack contains 5 vials of 0.8 mls. Supplied in: Ultrapure water as a sodium salt at pH 7.5.
Compatible with all NEB DNA polymerases in a variety of applications
>= 99% pure as determined by HPLC analysis
Exceptional stability
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