RNAi and RNA Reagents
HORIZON DISCOVERY BIOSCIENCES LIMITED ON-TARGETplus 2.0 Human PRMT5 (10419) siRNA - SMARTpool, Unit Size: 5 nmol
This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015].
Apexbio Technology LLC ARCA, 10ul (100 mM). Cas: MFCD: NA. Anti Reverse Cap Analog (ARCA) used to forming Cap 0 structure，3´-O-Me-m7G(5')ppp(5')G
A critical step in mRNA processing is the addition of a 5 cap structure, a 5'-5' triphosphate linkage between the 5' initiating terminal of the RNA and a guanosine nucleotide. The cap is then methylated enzymatically at the N-7 position of the guanosine to form a mature mCAP. Other sizes are available. Please inqury us for quote.
Apexbio Technology LLC mCAP, 100ul (100 mM). Cas: MFCD: NA. m7G(5')ppp(5')G Cap Analog, used to forming Cap 0 structure
mRNA of eucaryon and virus has a monomethylated cap structure, m7G(5')ppp(5')G or called mCAP, at the 5-initiating terminal. Other sizes are available. Please inqury us for quote.
Apexbio Technology LLC ARCA EGFP mRNA, 5x1mg (1mg/mL). Cas: MFCD: NA. Direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells.
EGFP (enhanced green fluorescent protein) mRNA can express an enhanced green fluorescence once has been transfected into cells, which makes it always used as a direct detection reporter mRNA. You can observe the fluorescence at 509 nm. Our product had already been modified with ARCA with a high efficient co-transcriptional capping method, which means most of mRNA has a Cap 0 structure. It is a more mature mRNA than mRNA with no capping and maintain a more stable state which lead to higher expressing efficiency in transfected mammalian cells. ARCA obtain the ability only inserting in the proper orientation, results in forming mRNAs that can be translated twice efficiently as those initiated with mCAP. Other sizes are available. Please inqury us for quote.
HORIZON DISCOVERY BIOSCIENCES LIMITED siGENOME Non-Targeting siRNA Control Pool #1 | 20 nmol
siGENOME Non-Targeting siRNA Control Pools are highly functional, chemically synthesized, and mostly unmodified negative control siRNA duplexes for experiments in human mouse and rat cells. siGENOME Non-Targeting Control Pools are each a pool of four siRNAs, allowing the effective concentration of each siRNA to be reduced, and thus further minimize off-target effects. siGENOME Non-Targeting Control siRNAs were designed to have a minimum of 4 mismatches to all human, mouse and rat genes, and confirmed to have minimal targeting by genomewide microarray analysis. siGENOME Non-Targeting Pool #1 is comprised of Non-Targeting siRNAs #1 through 4.
HORIZON DISCOVERY BIOSCIENCES LIMITED EDIT-R SYNTHETIC SGRNA NONTAR
Edit-R Synthetic sgRNA Non-targeting Control #4, 5 nmol