shRNA
shRNA
HORIZON DISCOVERY BIOSCIENCES LIMITED GIPZ LENTIVIRAL SHRNA CONTROLS
NC2309311 GIPZ LENTIVIRAL SHRNA CONTROLS
OriGene TET2 Human shRNA Plasmid Kit (Locus ID 54790)
TET2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
OriGene pGFP-A-shAAV shRNA Vector | 5ug
The pGFP-A-shAAV vector was created with an integrated turboGFP element to readily verify transfection efficiency. It incorporates both a ampicillin and puromycin resistance elements for greater selection capabilities. The AAV2-backbone vector enables the helper-free packaging method to produce AAV particles.
HORIZON DISCOVERY BIOSCIENCES LIMITED PROMOTER: CAG EDIT-R LENTIVIRA
promoter: CAG Edit-R Lentiviral Blast-Cas9 Nuclease Particles
HORIZON DISCOVERY BIOSCIENCES LIMITED INDUCIBLE CAS9 PLASMID 10UG
Lentiviral Cas9 nuclease reagentInduciblePlasmid DNA 10ug
HORIZON DISCOVERY BIOSCIENCES LIMITED SMART VECTOR IND LENTI VRL4/PK
NC1956484 SMART VECTOR IND LENTI VRL4/PK
HORIZON DISCOVERY BIOSCIENCES LIMITED SMARTVECTOR IND HU CNOT7 PGK
Set of 3 SMARTvector Inducible Human CNOT7 PGK-TurboRFP shRNA, 100 μL, 10^7 TU/mL
Revvity Health Sciences Inc The DepleteX High Expressing RNA Depletion kit selectively removes the most highly expressed transcripts in vitro before sequencing.
Improve detection sensitivity in fibroblasts and whole blood.While resequencing highly expressed transcripts contributes minimally to biologically relevant information, these transcripts dominate sequencing coverage, hindering detection of low-expressing genes, rare variants, and unique fusion events associated with diseases.DepleteX utilizes Cas9 and a guide RNA set to selectively remove 70% of the most highly expressed transcripts from poly-A selected RNA-Seq libraries obtained from fibroblasts and whole blood. This design optimizes the reallocation of sequencing reads, enhancing coverage of rare, low-expressing transcripts and unique fusion events.The DepleteX High Expressing RNA Depletion kit is used during the RNA-seq library prep workflow starting with fibroblasts and whole blood samples after the ligation step and before the PCR step. This kit is compatible with most RNA-seq workflows; however, we recommend using it with the NEXTFLEX Rapid Directional RNA-seq kit 2.0.