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Short/small hairpin RNA (shRNA) is an artificial RNA molecule with a tight hairpin turn used to silence target gene expression via RNA interference (RNAi). Cells can be made to express shRNA through the introduction of plasmids or viral/bacterial vectors.
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This gene encodes a receptor for prostaglandin E2, a metabolite of arachidonic acid which has different biologic activities in a wide range of tissues. Mutations in this gene are associated with aspirin-induced susceptibility to asthma.
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Lentiviral Packaging Mix is a ready-to-use third generation HIV-based lentiviral packaging system in which the plasmids express the elements required for lentiviral production. To produce high quality lentiviral particles, all you need is a lentiviral expression vector containing your gene of interest. Lentiviral Packaging Mix contains the essential packaging vectors containing the viral structure proteins and the envelope vector to express the Vesicular Stomatitis Virus glycoprotein (VSVG). The lentiviral packaging mix is TAT-independent because it only supports lentiviral expression vector with a chimeric 5' LTR, in which the HIV promoter is replaced with CMV or RSV.
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h/m P53 shRNA Set (GFP Puro) shRNA lentiviral vector expressing GFP with Puromycin selection for studies in Human/Mouse Each vial provides a nominal titer of 5x10E6 (sh-mix) 5x10E6 (scr-mix) transducing units TU Validated human P53 shRNA Lentivirus High-titer shRNA lentiviral particles specific for human tumor suppressor protein p53 (P53/TP53) The shRNA has been validated to meet or exceed 70% P53 knockdown efficiency using a specific fluorescence-based method that is more rapid and reliable than qPCR The shRNA lentivirus is ultra-purified and concentrated to high-titer by PEG precipitation and sucrose gradient centrifugation and ideal for transducing difficult-to-transfect cells including thawed and/or primary cells Order a shRNA lentivirus set and receive lentivirus produced from mix of 2 independent shRNAs validated to knockdown the target gene ( 70%) plus control lentivirus produced from mix of 2 scrambled shRNA constructs
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More