miRNA
Apexbio Technology LLC ARCA EGFP mRNA, 5x1mg (1mg/mL). Cas: MFCD: NA. Direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells.
EGFP (enhanced green fluorescent protein) mRNA can express an enhanced green fluorescence once has been transfected into cells, which makes it always used as a direct detection reporter mRNA. You can observe the fluorescence at 509 nm. Our product had already been modified with ARCA with a high efficient co-transcriptional capping method, which means most of mRNA has a Cap 0 structure. It is a more mature mRNA than mRNA with no capping and maintain a more stable state which lead to higher expressing efficiency in transfected mammalian cells. ARCA obtain the ability only inserting in the proper orientation, results in forming mRNAs that can be translated twice efficiently as those initiated with mCAP. Other sizes are available. Please inqury us for quote.
PROTEINONE Hela Nuclear Extract for pre-mRNA splicing, 200 ug
This HeLa cell nuclear extract is specifically recommended for: 1) in vitro splicing; 2) protein-DNA/RNA and protein-protein interactions; and 3) source of individual splicing factors. Although this extract also contains most transcription factors, it is, however, not recommended for in vitro transcription assays.
Apexbio Technology LLC SpCas9 mRNA (ARCA, 5mCTP, UTP), 5x1mg (1mg/mL)
SpCas9 mRNA expresses an optimized version of Streptococcus pyogenes protein. It can be used with a purified guideRNA sequence for genomic DNA target recognition and cleavage in the CRISPR-Cas genome editing system. Type II CRISPR-Cas system from the bacterium S. pyogenes has been adapted for genome engineering in eukaryotic systems, which requires two main components: the endoculease Cas9 and non-coding guide RNA (gRNA). The Cas9 protein can be programmed with gRNA, targeting a site-specific DNA double-strand break (DSB). Other sizes are available. Please inqury us for quote.
HORIZON DISCOVERY BIOSCIENCES LIMITED CRISPRA DCAS9-VPR MRNA EGFP 20
CRISPRa dCas9-VPR mRNA egfp 20ug
Apexbio Technology LLC mCAP, 10ul (100 mM). Cas: MFCD: NA. m7G(5')ppp(5')G Cap Analog, used to forming Cap 0 structure
mRNA of eucaryon and virus has a monomethylated cap structure, m7G(5')ppp(5')G or called mCAP, at the 5-initiating terminal. Other sizes are available. Please inqury us for quote.
Sciencell Research Laboratories SCIENCELL RESEARCH LABORATORIES INC
NC3957521 OLIGODENDROCYTE PRECURSOR CELL
TWD Scientific LLC BioX Wastewater Nucleic Acid Kit 50 Reactions/Kit
BioX Wastewater Nucleic Acid Kit 50 Reactions/Kit
Apexbio Technology LLC ARCA, 100ul (100 mM). Cas: MFCD: NA. Anti Reverse Cap Analog (ARCA) used to forming Cap 0 structure，3´-O-Me-m7G(5')ppp(5')G
A critical step in mRNA processing is the addition of a 5 cap structure, a 5'-5' triphosphate linkage between the 5' initiating terminal of the RNA and a guanosine nucleotide. The cap is then methylated enzymatically at the N-7 position of the guanosine to form a mature mCAP. Other sizes are available. Please inqury us for quote.
Apexbio Technology LLC mCAP, 50ul (100 mM). Cas: MFCD: NA. m7G(5')ppp(5')G Cap Analog, used to forming Cap 0 structure
mRNA of eucaryon and virus has a monomethylated cap structure, m7G(5')ppp(5')G or called mCAP, at the 5-initiating terminal. Other sizes are available. Please inqury us for quote.
Apexbio Technology LLC ARCA, 50ul (100 mM). Cas: MFCD: NA. Anti Reverse Cap Analog (ARCA) used to forming Cap 0 structure，3´-O-Me-m7G(5')ppp(5')G
A critical step in mRNA processing is the addition of a 5 cap structure, a 5'-5' triphosphate linkage between the 5' initiating terminal of the RNA and a guanosine nucleotide. The cap is then methylated enzymatically at the N-7 position of the guanosine to form a mature mCAP. Other sizes are available. Please inqury us for quote.