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MicroRNAs (miRNAs) are small, non-coding RNA molecules found in plants, animals, and some viruses that function in RNA silencing and post-transcriptional regulation of gene expression via base-pairing with complementary sequences within mRNA molecules.
Kit for microRNA quantitation and microRNA synthesis. Quantify any miRNA and its target by qPCR, using the same RNA sample. Simple, single-step cDNA synthesis reaction.
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A critical step in mRNA processing is the addition of a 5 cap structure, a 5'-5' triphosphate linkage between the 5' initiating terminal of the RNA and a guanosine nucleotide. The cap is then methylated enzymatically at the N-7 position of the guanosine to form a mature mCAP. Other sizes are available. Please inqury us for quote.
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hsa-miR-155-5p mimics are small chemically synthesized double-stranded RNAs that mimic endogenous miRNAs and enable miRNA functional analysis by up-regulation of miRNA activity
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MicroRNAs are natural regulators of gene expression affecting almost every cellular process (growth and development cell differentiation cell death). They occur naturally in diverse animal and plant species and hundreds have been discovered since the late 1990s. A single miRNA can ramp down expression of multiple genes. Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants.Capacity The High Pure Spin filter Tubes hold up to 700 muL sample volume.
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This HeLa cell nuclear extract is specifically recommended for: 1) in vitro splicing; 2) protein-DNA/RNA and protein-protein interactions; and 3) source of individual splicing factors. Although this extract also contains most transcription factors, it is, however, not recommended for in vitro transcription assays.
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hsa-miR-26b-5p mimics are small chemically synthesized double-stranded RNAs that mimic endogenous miRNAs and enable miRNA functional analysis by up-regulation of miRNA activity
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hsa-miR-150-5p inhibitors are chemically-modified oligonucleotides that hybridize with mature miRNAs The miRNA inhibitors have full-length nucleotide 2 -methoxy modification The miRNA inhibitors strongly compete with mature miRNAs to prevent the complementary pairing of miRNAs and their target genes thereby inhibiting miRNAs from functioning
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