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The FLuc mRNA will express a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
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ER-EGFP mRNA encodes a fusion protein comprising an endoplasmic reticulum (ER) targeting signal sequence and enhanced green fluorescent protein (EGFP) EGFP is a widely used fluorescent marker protein for biological imaging By fusing EGFP to the ER signal sequence dynamic changes in the ER can be observed in real-time in live cells making it particularly useful for studying the structure function and interactions of the ER with other cellular organelles ER-EGFP s efficient fluorescent properties and specific localization to the ER position it as an ideal tool for studying ER-related processes The EZ Cap ER-EGFP Probe mRNA (m1 ) is provided at 1 mg/mL specifically designed for targeted expression of a green fluorescent protein label at the ER This mRNA is synthesized through co-transcriptional capping resulting in a Cap 1 structure The incorporation of m1 modifications poly(A) tail significantly enhances mRNA stability and lifetime both in vitro and in vivo
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