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Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
Clone repetitive sequences and lentiviral librariesStabilize direct repeats and create lentiviral constructsGenerate CRISPR-GeCKO-Libraries-Endura lentiviral guide RNA librariesRecommended in CRISPR GeCKO library protocolsChoose electrocompetent or chemically competent cellsHighest efficiency commercially available cells for lentiviral cloningEndura™ Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube) or DUOs (2 transformations per tube) as indicated. Endura Electrocompetent cells are provided in DUOs format only. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
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Additionally, it does not require use of any potentially interferingcomponents such as detergents, commonly found in various lytic buffers.Highlights• Simple and controlled autolysis of E. coli• Strains can by lysed in minutes after harvesting• Method compatible with most buffer systems• Ideal for protein expression and purification, also applicable for extraction of nucleic acids• Scale up to lyse more samples without increase in time or labor
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Chemically competent E. coli cells suitable for transformation and protein expression. BL21 (DE3) Competent E. coli is a widely used T7 expression E. coli strain.
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Origami B host strains are similar as the original Origami strain, except that they are derived from a lacZY mutant of BL21. Strain carries the pLacI plasmid producing extra Lac repressor.
Vitamin B Complex 150 in indicated as a source of B complex vitamins for use in the treatment of deficiencies of these vitamins in cattle, sheep and horses.
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An activator of the anticipatory unfolded protein response inhibits protein synthesis and induces intracellular ATP depletion in T47D cells at 1 UM increases levels of phosphorylated EIF2AK3 eIF2a and AMPK in T47D cells at 1 UM cytotoxic to T47D TYS BT474 and ZR-75-1 ERa-positive breast cancer cells (IC50s 16.4 11.1 26.9 and 42.8 nM respectively) induces tumor regression in an MCF-7 mouse xenograft model at 40 mg/kg induces tumor regression in an ST941/HI PDX mouse model
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