Competent Cells
MilliporeSigma™ Novagen™ BL21 Competent Cell Set
BL21 is the most widely used host background and has the advantage of being deficient in both lon (1) and ompT proteases.
MilliporeSigma™ Novagen™ Rosetta™ 2 (DE3) Singles Competent Cells
Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.
MilliporeSigma™ Novagen™ Origami™ 2 (DE3) Competent Cells
Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.
MilliporeSigma™ Novagen™ Tuner™ Competent Cell Set
Tuner™ strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture.
MilliporeSigma™ Novagen™ RosettaBlue™ (DE3) Competent Cells
RosettaBlue host strains are NovaBlue derivatives that combine high transformation efficiency and with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
MilliporeSigma™ Novagen™ Rosetta-gami™ 2 (DE3) Competent Cells
Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
MilliporeSigma™ Novagen™ BL21(DE3) pLysS Competent Cells
BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.
MilliporeSigma™ Novagen™ Veggie BL21 (DE3) Singles™ Competent Cells
Protease-deficient hosts (BL21) for protein expression; Manufactured free of animal-derived media and components
MilliporeSigma™ Novagen™ Tuner™ DE3 pLysS Competent Cells
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. T7 lysozyme expression suppresses basal T7 expression.
MilliporeSigma™ Novagen™ NovaBlue Competent Cells
NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.
MilliporeSigma™ Novagen™ Rosetta™ Blue Competent Cells
RosettaBlue host strains are NovaBlue derivatives that combine high transformation efficiency and with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
MilliporeSigma™ Novagen™ DE3 Competent Cell Set
For Protein Expressions
MilliporeSigma™ Novagen™ Rosetta-gami™ 2 Competent Cell Set
Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.
MilliporeSigma™ Novagen™ Rosetta-gami™ B (DE3) Competent Cells
Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.
MilliporeSigma™ Novagen™ Origami™ B (DE3) Competent Cells
Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.