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Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
MultiShot StripWell BL21 Star (DE3) chemically competent E. coli cells are cloning competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems.
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MultiShot FlexPlate Mach1 T1R chemically competent E. coli cells are exceptionally fast-growing cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity.
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Rosetta-gami B strains combines the key features of BL21, Origami, and Rosetta to enhances the expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.
Rosetta-Gami 2 strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins. Contains the pLacI plasmid producing extra Lac repressor.
BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.
Small and Specialty Supplier Partner Small and/or specialty supplier based on Federal laws and SBA requirements. Learn More
NEB 5-alpha Competent E. coli (High Efficiency) is a derivative of the popular DH5. It is T1 phage resistant and endA deficient for high-quality plasmid preparations.
High efficiency strain ideal for a wide variety of applications
In a wide variety of sizes, including single-use vials, 200 µl vials, 96- and 384-well plates
No dry ice surcharge on competent cell shipments
Outgrowth medium included
Free of animal products
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Useful host for transformation of pGEM Vectors and production of ssDNA from M18 or phagemid vectors. JM109 is recA- and lacks the E. coli K restriction system. Can be used for blue/white screening.
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Used for high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, if the sequence contains a ribosomal binding site. No blue/white selection.
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More