Promega Bacterial Strain JM109(DE3)

Description

Used for high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, if the sequence contains a ribosomal binding site. No blue/white selection.

• For High-Level Gene Expression from the T7 Promoter
• Contains an IPTG-inducible gene for T7 RNA polymerase
• Requires a ribosomal binding site with cloned sequence
• Lacks blue/white cloning selection

Specifications

• Content And Storage: <-65°C
• For Use With (Application): For the high level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter
• Quantity: 500 μL

• Usage restrictions apply to Bacterial Strains JM109(DE3), BL21(DE3)pLysS and KRX and to any derivatives thereof. Please read the statement below describing these restrictions before purchasing any of these products.
• Usage Restrictions for the T7 Expression System
• The T7 expression system is based on technology developed at Brookhaven National Laboratory under contract with the U.S. Department of Energy and is the subject of patents assigned to Brookhaven Science Associates, LLC (BSA). This technology, including bacteria, phage and plasmids that carry the gene for T7 RNA polymerase, is to be used for academic or nonprofit laboratory or licensed commercial research purposes only. By accepting or using the T7 expression technology you agree to be bound by the following conditions set forth by BSA. The initial purchaser may refuse to accept the conditions of this notice by returning this product and the enclosed materials to Promega unused.
• Academic and Nonprofit Laboratories
• No materials that contain the cloned gene for T7 RNA polymerase may be distributed further to third parties outside of your laboratory unless the recipient receives a copy of this assurance notice and agrees to be bound by its terms. This limitation applies to Bacterial Strains JM109(DE3), BL21(DE3)pLysS and KRX and to any derivatives thereof.
• Commercial Laboratories
• A license is required for any commercial use of the T7 expression system, including use of the T7 system for research purposes or for production purposes by any commercial entity. Information about commercial licenses may be obtained from the Licensing Office, Brookhaven National Laboratory, Upton, NY 11973, Telephone: 631-344-7134, Fax: 631-344-3729.