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Promega Bacterial Strain JM109(DE3)

Used for high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, if the sequence contains a ribosomal binding site. No blue/white selection.

Manufacturer:  Promega P9801

Catalog No. PR-P9801


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Description

Description

  • For High-Level Gene Expression from the T7 Promoter
  • Contains an IPTG-inducible gene for T7 RNA polymerase
  • Requires a ribosomal binding site with cloned sequence
  • Lacks blue/white cloning selection
Specifications

Specifications

500 μL
For the high level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter
Safety and Handling

Safety and Handling

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Usage restrictions apply to Bacterial Strains JM109(DE3), BL21(DE3)pLysS and KRX and to any derivatives thereof. Please read the statement below describing these restrictions before purchasing any of these products.
Usage Restrictions for the T7 Expression System
The T7 expression system is based on technology developed at Brookhaven National Laboratory under contract with the U.S. Department of Energy and is the subject of patents assigned to Brookhaven Science Associates, LLC (BSA). This technology, including bacteria, phage and plasmids that carry the gene for T7 RNA polymerase, is to be used for academic or nonprofit laboratory or licensed commercial research purposes only. By accepting or using the T7 expression technology you agree to be bound by the following conditions set forth by BSA. The initial purchaser may refuse to accept the conditions of this notice by returning this product and the enclosed materials to Promega unused.
Academic and Nonprofit Laboratories
No materials that contain the cloned gene for T7 RNA polymerase may be distributed further to third parties outside of your laboratory unless the recipient receives a copy of this assurance notice and agrees to be bound by its terms. This limitation applies to Bacterial Strains JM109(DE3), BL21(DE3)pLysS and KRX and to any derivatives thereof.
Commercial Laboratories
A license is required for any commercial use of the T7 expression system, including use of the T7 system for research purposes or for production purposes by any commercial entity. Information about commercial licenses may be obtained from the Licensing Office, Brookhaven National Laboratory, Upton, NY 11973, Telephone: 631-344-7134, Fax: 631-344-3729.