Protein Labeling Reagents
Biotium LysoView™ 405, 1000X in DMSO, Trial Size
LysoView™ 405 is a blue fluorescent lysosome stain for live cells. It has excitation at 318 and 400 nm and emission at 464 nm, for detection in the DAPI or Pacific Blue channels.
Biotium Phalloidin, CF488A, 300 U
Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Freen fluorescent CF488A has excitation/emission at 490/515 nm.
Aat Bioquest HIS Lite™ Cy5 Bis NTA-Ni Complex
Polyhistidine is one of the most popular affinity tags incorporated into recombinant proteins. It can be inserted either at the N- or C-terminus, and expressed in a variety of hosts.
Biotium Tyramide Amplification Kit with HRP Streptavidin and Biotin-XX Tyramide
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. The kit contains HRP streptavidin Biotin-XX tyramide. The kit contains sufficient reagent for 50-150 slides.
Aat Bioquest HIS Lite™ Cy3 Bis NTA-Ni Complex
Polyhistidine is one of the most popular affinity tags incorporated into recombinant proteins. It can be inserted either at the N- or C-terminus, and expressed in a variety of hosts.
Biotium CF568 Tyramide
CF dye tyramide conjugates can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF568 dye has excitation/emission at 562/583 nm.Also see Biotium's CF Dye Tyramide Amplification Kits.
Biotium CF405L Succinimidyl Ester
CF Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. 405 nm-excitable green fluorescent CF405L dye has excitation/emission at 395/545 nm.
Biotium CF700 Succinimidyl Ester
CF Dye Succinimidyl Esters (SE, also known as NHS esters) are Biotium's next-generation fluorescent dyes in amine-reactive form for labeling antibodies and other proteins on amine groups, such as lysine residues. Near-infrared CF700 has excitation/emission at 695/720 nm.
Biotium CF488A Hydrazide
Our CF dye hydrazides are bright, extremely water-soluble and nontoxic. These properties make the dyes excellent fixable polar tracers for microinjection into cells. Hydrazides also can be used for labeling aldehyde or ketone groups, however, for this application we recommend using the more reactive CF dye aminooxy forms. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF488A dye has excitation/emission at 490/515 nm.
Biotium FAM SE, 5 and 6 isomers
5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate. Properties: Ex / Em = 495/519 nm; Yellow solid soluble in DMSO or DMF; Store at -20°C and protect from light; C 25 H 15 NO 9; MW: 473.4; [117548-22-8]
Biotium CF647 Tyramide
CF dye tyramide conjugates can be used for tyramide signal amplification (TSA), which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF647 dye has excitation/emission at 650/665 nm.
Lumiprobe Sulfo-Cyanine5 azide, 5 mg
Water-soluble Cyanine5 dye azide for Click chemistry. Bright and photostable dye with red fluorescence. The reagent has high water solubility. Due to high solubility and hydrophilicity, the dye can be used for labeling biomolecules in native, purely aqueous conditions.
Biotium CF633 Protein Labeling Kit , 3x(1mg) labelings
This kit contains everything you need to label antibodies or other proteins with a fluorescent CF dye succinimidyl ester (CF dye SE). Each kit contains reagents for three labeling reactions of up to 1 mg protein per reaction. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF633 dye has excitation/emission at 630/650 nm.
Lumiprobe Sulfo-Cyanine7 NHS ester, 1603861-95-5, 5mg
Molecular formula: C41H46N3KO10S2, Molecular weight: 844.05, Appearance: dark green powder, Solubility: good in water, DMF, DMSO; Sulfo-Cyanine7 is a water-soluble near infrared dye. The fluorophore is ideal for the labeling of complex proteins for subsequent live organism NIR imaging studies or sonoluminescence.
