Protein Labeling Reagents
Biotium Aminooxy-Biotin
Aminooxy-biotin can be used to covalently attach biotin to a molecule with an aldehyde or ketone group. The aminooxy group reacts with an aldehyde or ketone group to form a stable oxime linkage in an aqueous solution at neutral pH. The reaction is fast and can be further accelerated by adding aniline as a catalyst. Thus, aminooxy reagents are superior to hydrazide reagents, which also react with aldehydes or ketones but form unstable hydrazone linkages.
Biotium 5-CARBOXYTetramethylrhodamine, SINGLE IS
Free acid form of 5-TAMRA (5-Carboxytetramethylrhodamine, single isomer). Properties: Ex/ Em (MeOH) = 540/565 nm; Dark red solid soluble in DMSO, DMF, or MeOH C 25 H 22 N 2 O 5; MW: 430.5; [91809-66-4]
Biotium Sulforhodamine 101-PEO3-amine (Texas Red-PEO3-amine)
Sulforhodamine 101-PEO 3 -amine has a long water-soluble spacer separating the dye and the amine group. The dye can be coupled to activated carboxy groups or sulfonyl chlorides.Properties: Ex/Em (MeOH) = 583/603 nm; Dark purple solid soluble in DMSO or DMF; Store at 4°C and protect from light; C 39 H 48 N 4 O 9 S 2; MW: 780.95
Biotium 5-(AND-6)-CARBOXY-X-Rhodamine, SUCCINIMI
5(6)-ROX, SE (full name: 5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester) is an amine-reactive long wavelength rhodamine dye. Properties: Ex/Em (MeOH) = 568/595 nm; Dark red solid soluble in DMSO or DMF; Store at -20°C and protect from light; C 37 H 33 N 3 O 7; MW: 631.7; [114616-32-9].
Aat Bioquest 6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester] *Single Isomer*
6-HEX, SE is the amine-reactive succinimidyl ester of 6-HEX acid. It is widely used in nucleic acid sequencing and related research.
Biotium Eosin-5-isothiocyanate
Eosin-5-isothiocyanate is a good photosensitizer and can be used to selectively label amines. It has a quantum yield of 0.57 for singlet oxygen generation, which makes it useful for photoconversion of electron-rich materials such as diaminobenzidine (DAB) or fluorescent dyes into highly electron-dense materials for high resolution electron microscopy studies. Additionally, eosin is a good fluorescence acceptor for fluorescein (FAM), dansyl and coumarin dyes in fluorescence resonance energy transfer (FRET). Properties: Ex/Em (pH 9) = 521/544 nm; e = 95,000; Orange red solid soluble in DMF or DMSO; Store at -20°C and protect from light; C 21 H 7 Br 4 NO 5 S; MW: 704.96