Protein Labeling Reagents
Biotium CellBrite™ Steady 488 Membrane Staining Kit | 100 labelings
CellBrite™ Steady Membrane Staining Kits allow fluorescence imaging of cell surface for up to several days in culture. The CellBrite™ Steady Dyes are unique fluorescent membrane probes that distribute between the cell surface and intracellular compartments, so cells retain cell surface staining over time. With the use of CellBrite™ Steady Enhancer, intracellular staining can be reduced or eliminated for imaging of cell outlines or boundaries.
Lumiprobe Sulfo-Cyanine5 NHS ester, 2230212-27-6, 1mg
Molecular formula: C36H40N3KO10S2, Molecular weight: 777.95, Appearance: dark blue powder, Solubility: very good in water, good in DMF and DMSO; Water-soluble red emitting fluorescent dye. Amine-reactive Cy5 analog for the labeling of proteins/peptides and amino-oligonucleotides.
Biotium MitoView™ Green, Trial Size (50 UG)
MitoView™ dyes are membrane-permeant fluorogenic stains that rapidly accumulate in mitochondria of live cells. They can be imaged without washing, and are available with blue, green, far-red, and near-infrared fluorescence.MitoView™ Green is a green fluorescent mitochondrial dye (Ex/Em 490/520 nm) similar to MitoTracker Green FM. The dye is non-fluorescent until it paritions into the mitochondrial membrane. The staining relies on mitochondrial mass, not on mitochondrial membrane potential (MMP) and can therefore be used in both live cells and fixed cells. Biotium also offers several classical dyes for measuring MMP including JC-1, a ratiometric dye that forms red aggregates in healthy cells and green monomers when MMP is lost; and TMRM and TMRE, red fluorescent dyes used to quantitatively measure mitochondrial membrane potential using the Nernst equation.MitoTracker is a registered trademark of Thermo Fisher Scientific.
Lumiprobe Sulfo-Cyanine5 NHS ester, 2230212-27-6, 5mg
Molecular formula: C36H40N3KO10S2, Molecular weight: 777.95, Appearance: dark blue powder, Solubility: very good in water, good in DMF and DMSO; Water-soluble red emitting fluorescent dye. Amine-reactive Cy5 analog for the labeling of proteins/peptides and amino-oligonucleotides.
Lumiprobe THPTA ligand | 760952-88-3 | MFCD27665386 | 100 mg
THPTA ligand | Purity: 95% | Mol Wt: 434.5 | 760952-88-3 | MFCD27665386 | 100 mg
Lumiprobe Cyanine5 NHS ester, 1263093-76-0, 25mg
Molecular formula: C36H42N3BF4O4, Molecular weight: 667.54, Appearance: dark blue solid, Solubility: very poorly soluble in water (0.19 mM = 127 mg/L), good in polar (DMSO, DMF) and chlorinated (DCM, chloroform) organic solvents; Amine-reactive red emitting fluorescent dye for the labeling of proteins/peptides and amino-oligonucleotides. For the labeling of sensitive proteins, use water-soluble sulfo-Cyanine5 NHS ester.
Dojindo Molecular Technologies Inc LysoPrime Green (10 μL x 1). High selectivity for lysosomes, high retention even in lysosomes that had their pH neutralized after staining, long-term visualization of lysosomes, Ex=456 nm, Ex=540 nm.
Understanding lysosome-related physiology requires lysosome-specific probes that can track the behavior of lysosomes within cells. As a consequence of being designed simply to target acidic environments, most lysosomal probes have poor specificity towards the lysosome and stain many non-lysosomal acidic compartments. Moreover, existing lysosome probes’ fluorescent intensity is highly reliant on the lysosomes’ pH; their emission intensity is unstable and varies significantly due to differences in acidity among lysosomes, even within a cell. (https://dojindo.com/lms-mapselectionguide/)
Lumiprobe Cyanine7 NHS ester | 2408482-09-5 | 25 mg
Cyanine7 NHS ester | Purity: 95% | Mol Wt: 733.64 | 2408482-09-5 | 25 mg
Active Motif Chromeo P503 Fluorogenic Dye, Amine-Reactive, Bright Color Change, 1 mg
Chromeo P503 is a fluorogenic dye that labels proteins and peptides by binding to primary amine groups, providing clear detection with its distinct blue to red color change. Its quantum yield dramatically increases upon conjugation, enabling effective biomolecule labeling suitable for diverse applications like gel electrophoresis and chromatography. This dye distinguishes itself with a significant shortwave spectral shift, minimizing background effects for reproducible results.
LICOR IRDye 800CW NHS Ester (5.0 mg)
IRDye infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence. These unique dyes are used in a variety of NIR imaging applications including Western blotting, plate-based assays, protein arrays, tissue section imaging, and molecular activity measurements. Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.