Protein Labeling Reagents
Enzo Life Sciences 5-FITC (ultra pure) (1g). CAS: 3326-32-7
Fluorescein isothiocyanate (FITC) is an amine-reactive fluorescein derivative that yields conjugates with superior stability. Dye synthesis generally results in two isomers that differ slightly in protein binding and may elute differently under chromatographic conditions, complicating analysis in certain situations. The 5-isomer of FITC is the most widely used isoform and has a multitude of applications beyond protein peptide and nucleotide labeling. For example, it is commonly employed as a donor for FRET (Fluorescence Resonance Energy Transfer) based assay systems. An acceptor molecule, such as TRITC, is recommended in such FRET applications. Wavelength Maxima: Excitation 494nm, Emission 520nm Alternative name: 5-Fluorescein Isothiocyanate. Purity: ≥95% (HPLC). Solubility: Soluble in DMSO. Long Term Storage: -20°C.
Sigma Aldrich Fine Chemicals Biosciences Sera MagTM magnetic carbox
Sera-Mag(TM) SpeedBeads(TM) and Sera-Mag(TM) Carboxylate-Modified Magnetic Particles combine a fast magnetic response time and high binding capacity with a large surface area high sensitivity stability physical integrity and fast reaction kinetics. Covalent coupling of biomolecules such as proteins and nucleic acids to carboxyl groups on the surface is easily accomplished using our standard coupling technology. The core of the particle is made by a free radical emulsion polymerization of styrene and acid monomer. One (Sera-Mag(TM)) or two (Sera-Mag(TM) SpeedBeads(TM)) layers of magnetite are then coated onto this core while the surface is modified to minimize non-specific binding of proteins.
Enzo Life Sciences Rhod-2 AM (ultra pure) (1mg). CAS: 129787-64-0
Rhod-2 AM has the longest fluorescent emission signal of the commonly used calcium indicators. It contains a rhodamine-like fluorophore, making it suitable for use with argon and krypton laser excitation sources. Rhod-2 signal intensity one of the strongest of all the calcium probes. Rhod 2 is especially suitable probe for intracellular calcium monitoring by confocal laser-scanning microscopy and flow cytometry. The dissociation constant of Rhod 2 with calcium is among the highest of all the fluorescent calcium probes, providing a wider range for monitoring calcium concentration. Wavelength Maxima: Excitation 549nm, Emission 578nm. Alternative name: 1-[2-Amino-5-(3-dimethylamino-6-dimethylammonio-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)]ethane-N,N,N',N'-tetraacetic acid, tetraacetoxy methyl ester . Br. Purity: ≥90% (HPLC). Solubility: Soluble in DMSO. Long Term Storage: -20°C.
Sigma Aldrich Fine Chemicals Biosciences Phalloidin Atto 565 suitab
Atto 565 is a novel fluorescent label that belongs to the class of Rhodamine dyes. It shows a strong absorption high fluorescence quantum yield high thermal and photostability and a very little triplet formation. Atto 565 consists of a mixture of two isomers with practically identical optical absorption and emission Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind f actin in liver and muscle cells. As a result of binding phalloidin actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10-8. Phalloidin differs from amanitin in rapidity of action at high dose levels death of mice or rats occurs within 1 or 2 hours. fluorescent conjugates of phalloidin are used to label actin filaments for histolo
Cederlane Amytracker 520, green fluorescent optotracer, 200 μL, 1 mg/mL, non-toxic, crosses blood-brain barrier, excitation 460 nm, emission 520 nm
Amytracker 520, green fluorescent optotracer, 200 μL, 1 mg/mL, non-toxic, crosses blood-brain barrier, excitation 460 nm, emission 520 nm
Sigma Aldrich Fine Chemicals Biosciences Atto 550 NHS ester BioReag1MG
ATTO Atto 550 NHS ester BioReag1MG
Sigma Aldrich Fine Chemicals Biosciences Nhs Mag SepharoseR Cytiva
NHS Mag Sepharose(R) are magnetic beads designed for pull-down techniques enabling rapid capture and enrichment of selected proteins based on affinity.