Protein Modification Reagents
Dojindo Molecular Technologies Inc Biotin Labeling Kit-NH2(3 SAMPLES), is used for preparation of biotin-labeled IgG, only requires 1 hour to have high recovery of conjugates, applicable for 50-200 UG IgG/sample.
Biotin Labeling Kit-NH2 is mainly used for the preparation of biotin-labeled IgG for enzyme immunoassay (EIA), NH2-reactive biotin, a component of this kit, has succinimidyl groups (NHS) that react with amino groups on proteins or other molecules. The labeling process is very simple, just add the NH2- reactive Biotin to IgG solution (50-200 UG protein/sample) and incubate at 37°C for 10 minutes. An average of 5 to 8 biotin molecules conjugate to each IgG molecule. The number of biotin molecules per protein can be determined by HABA assay. Excess biotin molecules can be removed by a filtration tube. The molecular weight of the protein to be labeled with this kit should be greater than 50,000. This kit contains all of the necessary reagents for labeling, including the storage buffer for conjugates. Dojindo has various protein labeling reagents. (https://www.dojindo.com/labeling-chemistry/)
Biolegend Recombinant Human FAP (carrier-free) Reactivity: Human; Apps: BA; Size: 500 ug
Recombinant Human FAP (carrier-free) Reactivity: Human; Apps: BA; Size: 500 ug
Supply Solutions Sigma Aldrich - Collagenase H, 100mg * Third Party Item
Sigma Aldrich - Collagenase H, 100mg * Third Party Item
Biotium MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) (MMP2/1501), Biotin conjugate, 0.1mg/mL
This antibody recognizes a protein of 72 kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV,V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specifi
Gold Biotechnology Inc Phenylmethylsulfonyl fluoride (PMSF) 25 g
PMSF is a serine protease inhibitor that irreversibly inhibits serine proteases by sulfonylation of the serine residue in the active site of the protease. It is widely used in protocols for the isolation of proteins.
Sigma Aldrich Fine Chemicals Biosciences Dispase II (neutral protease, grade II), 5 x 1 g
Dispase is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid, effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting cells already in culture to transfer to new substrate (secondary culture). Furthermore, it is used to prevent unwanted clumping of cells cultured in suspension.
Discovery Life Sciences Gastric Cancer MSI H DTC
DTC Dissociated Tumor and Tissue Cells start as fresh solid tumor tissue collected during surgery are enzymatically and mechanically dissociated into single cells and are delivered as viably cryopreserved cells for functional assays cell culture and single cell genomics
Vitacyte LLC CIzyme RI Lyophilized Collagenase
This item has a minimum qty of 2 per supplier requirements.
CIzyme RI is an aseptically filled, lyophilized mixture of 60% purified class I (C1) and 40% purified class II (C2) collagenase from Clostridium histolyticum pre-mixed with neutral protease from Bacillus polymyxa. Manufactured for superior lot-to-lot enzyme consistency for improved performance consistencyFormulated to contain an optimal amount of Collagen Degrading Activity (CDA) and Neutral Protease Activity (NPA) for rodent islet isolationLow contaminating endotoxin levels (<25 EU/OD)Lot specific Certificate of Analysis provided with complete QC analysis results.
Sigma Aldrich Fine Chemicals Biosciences Pepsin from porcine gastric mucosa lyophilized powder, >=2,500 units/mg protein (E1%/280) | 9001-75-6 | MFCD00081840 | 25G
Pepsin from porcine gastric mucosa lyophilized powder, >=2,500 units/mg protein (E1%/280) | 9001-75-6 | MFCD00081840 | 25G
Discovery Life Sciences Gastric Cancer MSS DTC
DTC Dissociated Tumor and Tissue Cells start as fresh solid tumor tissue collected during surgery are enzymatically and mechanically dissociated into single cells and are delivered as viably cryopreserved cells for functional assays cell culture and single cell genomics
ABclonal Technology EP400 Rabbit pAb
Predicted to enable several functions, including ATP binding activity, ATP-dependent chromatin remodeler activity, and protein antigen binding activity. Involved in histone H2A acetylation and histone H4 acetylation. Part of NuA4 histone acetyltransferase complex and Swr1 complex.
Supply Solutions Collagenase P from Clostridium histolyticum, 1G
Collagenase P from Clostridium histolyticum, 1G
Sigma Aldrich Fine Chemicals Biosciences Collagenase from Clostridium histolyticum for general use, Type I, 0.25-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid | 9001-12-1 | MFCD00130830 | 500mg
Collagenase from Clostridium histolyticum for general use, Type I, 0.25-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid | 9001-12-1 | MFCD00130830 | 500mg