Protein Modification Reagents
Thermo Scientific™ Pierce™ Alkylating Reagents
Alkylating reagents provided in single-use, pre-weighed formats to streamline protein preparation workflows.
Thermo Scientific™ Pierce™ DTT (Dithiothreitol), No-Weigh™ Format
Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds.
Thermo Scientific™ MS(PEG)4 Methyl-PEG-NHS-Ester Reagent
Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).
Thermo Scientific™ Pierce™ NEM (N-ethylmaleimide)
Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.
Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate
Amine-reactive (NHS ester) compound to permanently block primary amines (e.g., protein lysine side chains) to prevent interactions or later conjugation.
Thermo Scientific Chemicals Trypsin, porcine pancreas
CAS: 7-7-9002 Molecular Formula: C35H47N7O10 Synonym: EC 3.4.21.4; Trypsin IV porcine
Thermo Scientific™ Pierce™ Cysteine-HCl
Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl).
MilliporeSigma™ Calbiochem™ EDTA, Disodium Salt, Dihydrate, Molecular Biology Grade
Application in Molecular Biology
Thermo Scientific™ Pierce™ Sodium meta-Periodate
Gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization using NaIO4 (CAS 7790-28-5).
Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/μL)
Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.
Invitrogen™ Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)
Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)
Thermo Scientific™ Pierce™ Premium Grade TCEP-HCl
Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this disulfide reducing agent.
Invitrogen™ DTNB; Ellman's Reagent, 5,5'-Dithiobis-(2-Nitrobenzoic Acid)
DTNB; Ellman's Reagent, 5,5'-Dithiobis-(2-Nitrobenzoic Acid)
Thermo Scientific™ MT(PEG)4 Methyl-PEG-Thiol Compound
MT(PEG)n: methyl and sulfhydryl compound with a 4-unit polyethylene glycol (PEG) spacer; methylate and pegylate quantum dots, gold surfaces, silver.