Gene Reporter Systems

Reporter genes are attached to a regulatory sequence of a gene of interest. When expressed by the host organism, they generate measurable, easily identifiable characteristics that can be used as an indication of whether a gene has been taken up or expressed.

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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ TOP10 Chemically Competent E. coli

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

Pricing & Availability
Specifications

Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Bacterial or Yeast Strain	TOP10
Vector	TOPO-TA Cloning Vectors
Product Line	One Shot

Invitrogen™ NovaBright™ Phospha-Light™ EXP Assay Kit for SEAP (Secreted Placental Alkaline Phosphatase) Reporter Gene Detection

Chemiluminescent reporter gene assay for the ultrasensitive detection of secreted placental alkaline phosphatase or the non-secreted placental isozyme PLAP.

Pricing & Availability
Specifications

Substrate	CSPD™
Substrate Properties	Chemical Substrate
Content And Storage	Component solution containing CSPD™ substrate and chemiluminescence enhancer (Store at 2°C to 6°C), placental alkaline phosphatase positive control (Store at ≤ -20°C)
Format	96-well Plate
For Use With (Equipment)	Luminometer (Microplate)
Product Type	Phosphatase Assay
Target Enzyme	Placental Alkaline Phosphatase
Substrate Type	AP (Alkaline Phosphatase) Substrate
High-throughput Compatibility	High-throughput Compatible
Product Line	NovaBright
Detection Method	Enhanced Chemiluminescence

Promega Luciferase Reporter Assays to Study Nuclear Receptors: pFN26A (BIND) hRluc-neo Flexi™ Vector

NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.

Pricing & Availability

Invitrogen™ Alexa Fluor™ 647-aha-dCTP

For better resolution of two-color microarray gene expression assays

Pricing & Availability

Invitrogen™ FluoReporter™ lacZ/Galactosidase Quantitation Kit

Reaction produces blue fluorescent product

Pricing & Availability

Invitrogen™ Yeast tRNA

Supplied in lyophilized form

Pricing & Availability
Specifications

Shipping Condition	Approved for shipment at Room Temperature or on Wet or Dry Ice
Content And Storage	Store in freezer (-5 to -30°C).
Form	Lyophilized Powder
Product Type	Yeast tRNA
Reagent Type	Yeast tRNA
For Use With (Application)	Carrier for Nucleic Acid Precipitation